Enhanced oxidative stress is associated with tissue neutrophilia and poor steroid response in chronic rhinosinusitis with nasal polyps
Abstract Objective To analyze the oxidative stress status and its association with tissue neutrophilia and oral steroid response in chronic rhinosinusitis with nasal polyps (CRSwNP) patients. Methods The levels of total oxidant status (TOS) were detected in the sinonasal tissues by using specific as...
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | World Journal of Otorhinolaryngology-Head and Neck Surgery |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/wjo2.91 |
| _version_ | 1797404549294587904 |
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| author | Xian‐Ting Hu Bai‐Wen Chen Yu‐Jie Cao Chun Zhou Hua‐Bin Li De‐Hui Wang |
| author_facet | Xian‐Ting Hu Bai‐Wen Chen Yu‐Jie Cao Chun Zhou Hua‐Bin Li De‐Hui Wang |
| author_sort | Xian‐Ting Hu |
| collection | DOAJ |
| description | Abstract Objective To analyze the oxidative stress status and its association with tissue neutrophilia and oral steroid response in chronic rhinosinusitis with nasal polyps (CRSwNP) patients. Methods The levels of total oxidant status (TOS) were detected in the sinonasal tissues by using specific assay kits. Tissue neutrophil was examined by immunohistochemical staining, and oxidant status index (OSI) was evaluated in polyps tissues, and the messenger RNA (mRNA) levels of superoxide dismutase 2 (SOD2), aldehyde dehydrogenase 1 (ALDH1A1), and microsomal glutathione S‐transferase 1 (MGST1) were examined using quantitative real‐time polymerase chain reaction in the sinonasal tissues. The receiver operating characteristics (ROCs) curve of ALDH1A1, MGST1, and SOD2 mRNA levels were evaluated to determine the steroid response of CRSwNP patients. Results The levels of TOS and OSI were significantly higher in CRSwNP and CRSsNP than in normal controls, and OSI in polyps tissues was positively associated with tissue neutrophilia and poor steroid response. The ALDH1A1, MGST1, and SOD2 mRNA levels showed comparable accuracy as predictors of poor steroid response indicated by the area under the curve. Conclusion These findings provided evidence that the increased level of oxidative stress contributes to enhanced tissue neutrophilia and poor steroid response in CRSwNP patients. |
| first_indexed | 2024-03-09T02:56:37Z |
| format | Article |
| id | doaj.art-f490bf43f40749ff8ddb897de1a8c443 |
| institution | Directory Open Access Journal |
| issn | 2095-8811 2589-1081 |
| language | English |
| last_indexed | 2024-03-09T02:56:37Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | World Journal of Otorhinolaryngology-Head and Neck Surgery |
| spelling | doaj.art-f490bf43f40749ff8ddb897de1a8c4432023-12-05T06:26:41ZengWileyWorld Journal of Otorhinolaryngology-Head and Neck Surgery2095-88112589-10812023-12-019432032710.1002/wjo2.91Enhanced oxidative stress is associated with tissue neutrophilia and poor steroid response in chronic rhinosinusitis with nasal polypsXian‐Ting Hu0Bai‐Wen Chen1Yu‐Jie Cao2Chun Zhou3Hua‐Bin Li4De‐Hui Wang5Department of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai ChinaDepartment of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai ChinaDepartment of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai ChinaDepartment of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai ChinaDepartment of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai ChinaDepartment of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai ChinaAbstract Objective To analyze the oxidative stress status and its association with tissue neutrophilia and oral steroid response in chronic rhinosinusitis with nasal polyps (CRSwNP) patients. Methods The levels of total oxidant status (TOS) were detected in the sinonasal tissues by using specific assay kits. Tissue neutrophil was examined by immunohistochemical staining, and oxidant status index (OSI) was evaluated in polyps tissues, and the messenger RNA (mRNA) levels of superoxide dismutase 2 (SOD2), aldehyde dehydrogenase 1 (ALDH1A1), and microsomal glutathione S‐transferase 1 (MGST1) were examined using quantitative real‐time polymerase chain reaction in the sinonasal tissues. The receiver operating characteristics (ROCs) curve of ALDH1A1, MGST1, and SOD2 mRNA levels were evaluated to determine the steroid response of CRSwNP patients. Results The levels of TOS and OSI were significantly higher in CRSwNP and CRSsNP than in normal controls, and OSI in polyps tissues was positively associated with tissue neutrophilia and poor steroid response. The ALDH1A1, MGST1, and SOD2 mRNA levels showed comparable accuracy as predictors of poor steroid response indicated by the area under the curve. Conclusion These findings provided evidence that the increased level of oxidative stress contributes to enhanced tissue neutrophilia and poor steroid response in CRSwNP patients.https://doi.org/10.1002/wjo2.91chronic rhinosinusitisnasal polypsneutrophiloxidative stresssteroid response |
| spellingShingle | Xian‐Ting Hu Bai‐Wen Chen Yu‐Jie Cao Chun Zhou Hua‐Bin Li De‐Hui Wang Enhanced oxidative stress is associated with tissue neutrophilia and poor steroid response in chronic rhinosinusitis with nasal polyps World Journal of Otorhinolaryngology-Head and Neck Surgery chronic rhinosinusitis nasal polyps neutrophil oxidative stress steroid response |
| title | Enhanced oxidative stress is associated with tissue neutrophilia and poor steroid response in chronic rhinosinusitis with nasal polyps |
| title_full | Enhanced oxidative stress is associated with tissue neutrophilia and poor steroid response in chronic rhinosinusitis with nasal polyps |
| title_fullStr | Enhanced oxidative stress is associated with tissue neutrophilia and poor steroid response in chronic rhinosinusitis with nasal polyps |
| title_full_unstemmed | Enhanced oxidative stress is associated with tissue neutrophilia and poor steroid response in chronic rhinosinusitis with nasal polyps |
| title_short | Enhanced oxidative stress is associated with tissue neutrophilia and poor steroid response in chronic rhinosinusitis with nasal polyps |
| title_sort | enhanced oxidative stress is associated with tissue neutrophilia and poor steroid response in chronic rhinosinusitis with nasal polyps |
| topic | chronic rhinosinusitis nasal polyps neutrophil oxidative stress steroid response |
| url | https://doi.org/10.1002/wjo2.91 |
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