Evaluation of Silk Fibroin/Gellan Gum Hydrogels with Controlled Molecular Weight through Silk Fibroin Hydrolysis for Tissue Engineering Application
Hydrogel is a versatile material that can be manipulated to achieve the desired physicochemical properties, such as stiffness, pore size, and viscoelasticity. Traditionally, these properties have been controlled through parameters such as concentration and pH adjustments. In this study, we focused o...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/13/5222 |
| _version_ | 1827734745174245376 |
|---|---|
| author | Sunjae Park Soo-In Kim Joo-Hee Choi Se-Eun Kim Seung-Ho Choe Youngjun Son Tae-woong Kang Jeong-Eun Song Gilson Khang |
| author_facet | Sunjae Park Soo-In Kim Joo-Hee Choi Se-Eun Kim Seung-Ho Choe Youngjun Son Tae-woong Kang Jeong-Eun Song Gilson Khang |
| author_sort | Sunjae Park |
| collection | DOAJ |
| description | Hydrogel is a versatile material that can be manipulated to achieve the desired physicochemical properties, such as stiffness, pore size, and viscoelasticity. Traditionally, these properties have been controlled through parameters such as concentration and pH adjustments. In this study, we focused on exploring the potential of hydrolyzed silk fibroin (HSF) as a molecular weight-modulating agent to control the physicochemical properties of double-composite hydrogels. We developed a synergistic dual-crosslinked hydrogel by combining ionically crosslinked silk fibroin with gellan gum (GG). The hydrolysis of silk fibroin not only enhanced its hydrophilicity but also enabled adjustments in its mechanical properties, including the pore size, initial modulus elasticity, and relaxation time. Moreover, biocompatibility assessments based on cell viability tests confirmed the potential of these hydrogels as biocompatible materials. By highlighting the significance of developing an HSF/GG dual-crosslinked hydrogel, this study contributes to the advancement of novel double-composite hydrogels with remarkable biocompatibility. Overall, our findings demonstrate the capability of controlling the mechanical properties of hydrogels through molecular weight modulation via hydrolysis and highlight the development of a biocompatible HSF/GG dual-crosslinked hydrogel with potential biomedical applications. |
| first_indexed | 2024-03-11T01:33:48Z |
| format | Article |
| id | doaj.art-f491938f825c4be6b9f94669e5ffe992 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T01:33:48Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f491938f825c4be6b9f94669e5ffe9922023-11-18T17:10:00ZengMDPI AGMolecules1420-30492023-07-012813522210.3390/molecules28135222Evaluation of Silk Fibroin/Gellan Gum Hydrogels with Controlled Molecular Weight through Silk Fibroin Hydrolysis for Tissue Engineering ApplicationSunjae Park0Soo-In Kim1Joo-Hee Choi2Se-Eun Kim3Seung-Ho Choe4Youngjun Son5Tae-woong Kang6Jeong-Eun Song7Gilson Khang8Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaDepartment of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si 54896, Jeonbuk, Republic of KoreaHydrogel is a versatile material that can be manipulated to achieve the desired physicochemical properties, such as stiffness, pore size, and viscoelasticity. Traditionally, these properties have been controlled through parameters such as concentration and pH adjustments. In this study, we focused on exploring the potential of hydrolyzed silk fibroin (HSF) as a molecular weight-modulating agent to control the physicochemical properties of double-composite hydrogels. We developed a synergistic dual-crosslinked hydrogel by combining ionically crosslinked silk fibroin with gellan gum (GG). The hydrolysis of silk fibroin not only enhanced its hydrophilicity but also enabled adjustments in its mechanical properties, including the pore size, initial modulus elasticity, and relaxation time. Moreover, biocompatibility assessments based on cell viability tests confirmed the potential of these hydrogels as biocompatible materials. By highlighting the significance of developing an HSF/GG dual-crosslinked hydrogel, this study contributes to the advancement of novel double-composite hydrogels with remarkable biocompatibility. Overall, our findings demonstrate the capability of controlling the mechanical properties of hydrogels through molecular weight modulation via hydrolysis and highlight the development of a biocompatible HSF/GG dual-crosslinked hydrogel with potential biomedical applications.https://www.mdpi.com/1420-3049/28/13/5222silk fibroingellan gumdual-crosslinked hydrogelhydrolysistissue engineering |
| spellingShingle | Sunjae Park Soo-In Kim Joo-Hee Choi Se-Eun Kim Seung-Ho Choe Youngjun Son Tae-woong Kang Jeong-Eun Song Gilson Khang Evaluation of Silk Fibroin/Gellan Gum Hydrogels with Controlled Molecular Weight through Silk Fibroin Hydrolysis for Tissue Engineering Application Molecules silk fibroin gellan gum dual-crosslinked hydrogel hydrolysis tissue engineering |
| title | Evaluation of Silk Fibroin/Gellan Gum Hydrogels with Controlled Molecular Weight through Silk Fibroin Hydrolysis for Tissue Engineering Application |
| title_full | Evaluation of Silk Fibroin/Gellan Gum Hydrogels with Controlled Molecular Weight through Silk Fibroin Hydrolysis for Tissue Engineering Application |
| title_fullStr | Evaluation of Silk Fibroin/Gellan Gum Hydrogels with Controlled Molecular Weight through Silk Fibroin Hydrolysis for Tissue Engineering Application |
| title_full_unstemmed | Evaluation of Silk Fibroin/Gellan Gum Hydrogels with Controlled Molecular Weight through Silk Fibroin Hydrolysis for Tissue Engineering Application |
| title_short | Evaluation of Silk Fibroin/Gellan Gum Hydrogels with Controlled Molecular Weight through Silk Fibroin Hydrolysis for Tissue Engineering Application |
| title_sort | evaluation of silk fibroin gellan gum hydrogels with controlled molecular weight through silk fibroin hydrolysis for tissue engineering application |
| topic | silk fibroin gellan gum dual-crosslinked hydrogel hydrolysis tissue engineering |
| url | https://www.mdpi.com/1420-3049/28/13/5222 |
| work_keys_str_mv | AT sunjaepark evaluationofsilkfibroingellangumhydrogelswithcontrolledmolecularweightthroughsilkfibroinhydrolysisfortissueengineeringapplication AT sooinkim evaluationofsilkfibroingellangumhydrogelswithcontrolledmolecularweightthroughsilkfibroinhydrolysisfortissueengineeringapplication AT jooheechoi evaluationofsilkfibroingellangumhydrogelswithcontrolledmolecularweightthroughsilkfibroinhydrolysisfortissueengineeringapplication AT seeunkim evaluationofsilkfibroingellangumhydrogelswithcontrolledmolecularweightthroughsilkfibroinhydrolysisfortissueengineeringapplication AT seunghochoe evaluationofsilkfibroingellangumhydrogelswithcontrolledmolecularweightthroughsilkfibroinhydrolysisfortissueengineeringapplication AT youngjunson evaluationofsilkfibroingellangumhydrogelswithcontrolledmolecularweightthroughsilkfibroinhydrolysisfortissueengineeringapplication AT taewoongkang evaluationofsilkfibroingellangumhydrogelswithcontrolledmolecularweightthroughsilkfibroinhydrolysisfortissueengineeringapplication AT jeongeunsong evaluationofsilkfibroingellangumhydrogelswithcontrolledmolecularweightthroughsilkfibroinhydrolysisfortissueengineeringapplication AT gilsonkhang evaluationofsilkfibroingellangumhydrogelswithcontrolledmolecularweightthroughsilkfibroinhydrolysisfortissueengineeringapplication |