Laminin Adsorption and Adhesion of Neurons and Glial Cells on Carbon Implanted Titania Nanotube Scaffolds for Neural Implant Applications
Interfacing neurons persistently to conductive matter constitutes one of the key challenges when designing brain-machine interfaces such as neuroelectrodes or retinal implants. Novel materials approaches that prevent occurrence of loss of long-term adhesion, rejection reactions, and glial scarring a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/21/3858 |
| _version_ | 1797467008595394560 |
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| author | Jan Frenzel Astrid Kupferer Mareike Zink Stefan G. Mayr |
| author_facet | Jan Frenzel Astrid Kupferer Mareike Zink Stefan G. Mayr |
| author_sort | Jan Frenzel |
| collection | DOAJ |
| description | Interfacing neurons persistently to conductive matter constitutes one of the key challenges when designing brain-machine interfaces such as neuroelectrodes or retinal implants. Novel materials approaches that prevent occurrence of loss of long-term adhesion, rejection reactions, and glial scarring are highly desirable. Ion doped titania nanotube scaffolds are a promising material to fulfill all these requirements while revealing sufficient electrical conductivity, and are scrutinized in the present study regarding their neuron–material interface. Adsorption of laminin, an essential extracellular matrix protein of the brain, is comprehensively analyzed. The implantation-dependent decline in laminin adsorption is revealed by employing surface characteristics such as nanotube diameter, <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>ζ</mi></semantics></math></inline-formula>-potential, and surface free energy. Moreover, the viability of U87-MG glial cells and SH-SY5Y neurons after one and four days are investigated, as well as the material’s cytotoxicity. The higher conductivity related to carbon implantation does not affect the viability of neurons, although it impedes glial cell proliferation. This gives rise to novel titania nanotube based implant materials with long-term stability, and could reduce undesirable glial scarring. |
| first_indexed | 2024-03-09T18:47:32Z |
| format | Article |
| id | doaj.art-f491b411ad8c4564a2e6f4e67c5b2b15 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T18:47:32Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-f491b411ad8c4564a2e6f4e67c5b2b152023-11-24T06:10:20ZengMDPI AGNanomaterials2079-49912022-11-011221385810.3390/nano12213858Laminin Adsorption and Adhesion of Neurons and Glial Cells on Carbon Implanted Titania Nanotube Scaffolds for Neural Implant ApplicationsJan Frenzel0Astrid Kupferer1Mareike Zink2Stefan G. Mayr3Leibniz Institute of Surface Engineering (IOM), 04318 Leipzig, GermanyLeibniz Institute of Surface Engineering (IOM), 04318 Leipzig, GermanyResearch Group Biotechnology and Biomedicine, Faculty of Physics and Earth Sciences, Leipzig University, 04103 Leipzig, GermanyLeibniz Institute of Surface Engineering (IOM), 04318 Leipzig, GermanyInterfacing neurons persistently to conductive matter constitutes one of the key challenges when designing brain-machine interfaces such as neuroelectrodes or retinal implants. Novel materials approaches that prevent occurrence of loss of long-term adhesion, rejection reactions, and glial scarring are highly desirable. Ion doped titania nanotube scaffolds are a promising material to fulfill all these requirements while revealing sufficient electrical conductivity, and are scrutinized in the present study regarding their neuron–material interface. Adsorption of laminin, an essential extracellular matrix protein of the brain, is comprehensively analyzed. The implantation-dependent decline in laminin adsorption is revealed by employing surface characteristics such as nanotube diameter, <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>ζ</mi></semantics></math></inline-formula>-potential, and surface free energy. Moreover, the viability of U87-MG glial cells and SH-SY5Y neurons after one and four days are investigated, as well as the material’s cytotoxicity. The higher conductivity related to carbon implantation does not affect the viability of neurons, although it impedes glial cell proliferation. This gives rise to novel titania nanotube based implant materials with long-term stability, and could reduce undesirable glial scarring.https://www.mdpi.com/2079-4991/12/21/3858titania nanotubeslow-energy ion implantationlaminin adsorptionneurons and glial cell responsebiocompatibilityneural implant |
| spellingShingle | Jan Frenzel Astrid Kupferer Mareike Zink Stefan G. Mayr Laminin Adsorption and Adhesion of Neurons and Glial Cells on Carbon Implanted Titania Nanotube Scaffolds for Neural Implant Applications Nanomaterials titania nanotubes low-energy ion implantation laminin adsorption neurons and glial cell response biocompatibility neural implant |
| title | Laminin Adsorption and Adhesion of Neurons and Glial Cells on Carbon Implanted Titania Nanotube Scaffolds for Neural Implant Applications |
| title_full | Laminin Adsorption and Adhesion of Neurons and Glial Cells on Carbon Implanted Titania Nanotube Scaffolds for Neural Implant Applications |
| title_fullStr | Laminin Adsorption and Adhesion of Neurons and Glial Cells on Carbon Implanted Titania Nanotube Scaffolds for Neural Implant Applications |
| title_full_unstemmed | Laminin Adsorption and Adhesion of Neurons and Glial Cells on Carbon Implanted Titania Nanotube Scaffolds for Neural Implant Applications |
| title_short | Laminin Adsorption and Adhesion of Neurons and Glial Cells on Carbon Implanted Titania Nanotube Scaffolds for Neural Implant Applications |
| title_sort | laminin adsorption and adhesion of neurons and glial cells on carbon implanted titania nanotube scaffolds for neural implant applications |
| topic | titania nanotubes low-energy ion implantation laminin adsorption neurons and glial cell response biocompatibility neural implant |
| url | https://www.mdpi.com/2079-4991/12/21/3858 |
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