Conjugated linoleic acid modifies transcriptional cytokine profile and induces early specific secretory IgA response in Giardia lamblia infected mice
Objective(s): Adaptive immunity is crucial in controlling Giardia lamblia infection in the intestinal mucosa, and some dietary lipids may improve mucosal immune function. The aim of this study was to evaluate conjugated linoleic acid (CLA) on the Th17/Treg response and secretory IgA production in a...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2022-12-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_21158_a564f18bc37abfff0e0c5e0e38ff8b15.pdf |
| _version_ | 1797977646409187328 |
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| author | Itzel Reyes-Duarte Alexel Burgara-Estrella Lorena Bustamante-Cordova Silvia Moya-Camarena Héctor Parra-Sánchez Osiris Alvarez-Bajo Guillermo López-Cervantes Maricela Montalvo-Corral |
| author_facet | Itzel Reyes-Duarte Alexel Burgara-Estrella Lorena Bustamante-Cordova Silvia Moya-Camarena Héctor Parra-Sánchez Osiris Alvarez-Bajo Guillermo López-Cervantes Maricela Montalvo-Corral |
| author_sort | Itzel Reyes-Duarte |
| collection | DOAJ |
| description | Objective(s): Adaptive immunity is crucial in controlling Giardia lamblia infection in the intestinal mucosa, and some dietary lipids may improve mucosal immune function. The aim of this study was to evaluate conjugated linoleic acid (CLA) on the Th17/Treg response and secretory IgA production in a model of giardiasis infection.Materials and Methods: C3H/HeN male mice were infected with 5×106 G. lamblia trophozoites (GS/M-83-H7, ATCC collection). Mice were assigned randomly to experimental and control groups. CLA was administered to the experimental group and phosphate-buffered saline (PBS) was given to the control group. Parasite load kinetics was determined. Enzyme-linked immunosorbent assay (ELISA) was performed to evaluate IgA and cytokines. Nuclear transcription factors and cytokines were measured by RT-qPCR, and histology of small bowel cells was evaluated.Results: CLA administration reduced the parasite load (P<0.05) and increased early Giardia-specific secretory IgA production. CLA also increased the expression of interleukin-10, transforming growth factor (TGF)-β, and inducible nitric oxide synthase (iNOS) (P<0.05), while infection elevated the expression of Foxp3, with a peak at 40 days post-infection (P<0.05). There were no pathological changes in the colonic mucosa due to infection or treatment. Thus, CLA stimulated mucosal immunity and enhanced the humoral response against G. lamblia, not only for early infection control but also to promote regulatory cytokine production at 40 dpi, restoring the intestinal balance after parasite elimination.Conclusion: Our findings reveal novel anti-parasitic effects through the immune-modulatory activity of CLA against the intestinal parasite G. lamblia. |
| first_indexed | 2024-04-11T05:11:24Z |
| format | Article |
| id | doaj.art-f49736baf8ef432cbe0244982c974baa |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-11T05:11:24Z |
| publishDate | 2022-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-f49736baf8ef432cbe0244982c974baa2022-12-25T11:14:14ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742022-12-0125121468147610.22038/ijbms.2022.65796.1447121158Conjugated linoleic acid modifies transcriptional cytokine profile and induces early specific secretory IgA response in Giardia lamblia infected miceItzel Reyes-Duarte0Alexel Burgara-Estrella1Lorena Bustamante-Cordova2Silvia Moya-Camarena3Héctor Parra-Sánchez4Osiris Alvarez-Bajo5Guillermo López-Cervantes6Maricela Montalvo-Corral7Departamento de Nutrición y Metabolismo. Centro de Investigación en Alimentación y Desarrollo, A.C, Hermosillo, MéxicoDepartamento de Investigación en Física. Universidad de Sonora, Hermosillo, MéxicoDepartamento de Nutrición y Metabolismo. Centro de Investigación en Alimentación y Desarrollo, A.C, Hermosillo, MéxicoDepartamento de Nutrición y Metabolismo. Centro de Investigación en Alimentación y Desarrollo, A.C, Hermosillo, MéxicoDepartamento de Nutrición y Metabolismo. Centro de Investigación en Alimentación y Desarrollo, A.C, Hermosillo, MéxicoCONACYT-Departamento de Investigación en Física, Universidad de Sonora, Hermosillo, MéxicoDepartamento de Ciencias de la Salud. Universidad de Sonora. Hermosillo, MéxicoDepartamento de Nutrición y Metabolismo. Centro de Investigación en Alimentación y Desarrollo, A.C, Hermosillo, MéxicoObjective(s): Adaptive immunity is crucial in controlling Giardia lamblia infection in the intestinal mucosa, and some dietary lipids may improve mucosal immune function. The aim of this study was to evaluate conjugated linoleic acid (CLA) on the Th17/Treg response and secretory IgA production in a model of giardiasis infection.Materials and Methods: C3H/HeN male mice were infected with 5×106 G. lamblia trophozoites (GS/M-83-H7, ATCC collection). Mice were assigned randomly to experimental and control groups. CLA was administered to the experimental group and phosphate-buffered saline (PBS) was given to the control group. Parasite load kinetics was determined. Enzyme-linked immunosorbent assay (ELISA) was performed to evaluate IgA and cytokines. Nuclear transcription factors and cytokines were measured by RT-qPCR, and histology of small bowel cells was evaluated.Results: CLA administration reduced the parasite load (P<0.05) and increased early Giardia-specific secretory IgA production. CLA also increased the expression of interleukin-10, transforming growth factor (TGF)-β, and inducible nitric oxide synthase (iNOS) (P<0.05), while infection elevated the expression of Foxp3, with a peak at 40 days post-infection (P<0.05). There were no pathological changes in the colonic mucosa due to infection or treatment. Thus, CLA stimulated mucosal immunity and enhanced the humoral response against G. lamblia, not only for early infection control but also to promote regulatory cytokine production at 40 dpi, restoring the intestinal balance after parasite elimination.Conclusion: Our findings reveal novel anti-parasitic effects through the immune-modulatory activity of CLA against the intestinal parasite G. lamblia.https://ijbms.mums.ac.ir/article_21158_a564f18bc37abfff0e0c5e0e38ff8b15.pdfconjugated linoleic acidgiardia lambliail-10secretory igatgf-β |
| spellingShingle | Itzel Reyes-Duarte Alexel Burgara-Estrella Lorena Bustamante-Cordova Silvia Moya-Camarena Héctor Parra-Sánchez Osiris Alvarez-Bajo Guillermo López-Cervantes Maricela Montalvo-Corral Conjugated linoleic acid modifies transcriptional cytokine profile and induces early specific secretory IgA response in Giardia lamblia infected mice Iranian Journal of Basic Medical Sciences conjugated linoleic acid giardia lamblia il-10 secretory iga tgf-β |
| title | Conjugated linoleic acid modifies transcriptional cytokine profile and induces early specific secretory IgA response in Giardia lamblia infected mice |
| title_full | Conjugated linoleic acid modifies transcriptional cytokine profile and induces early specific secretory IgA response in Giardia lamblia infected mice |
| title_fullStr | Conjugated linoleic acid modifies transcriptional cytokine profile and induces early specific secretory IgA response in Giardia lamblia infected mice |
| title_full_unstemmed | Conjugated linoleic acid modifies transcriptional cytokine profile and induces early specific secretory IgA response in Giardia lamblia infected mice |
| title_short | Conjugated linoleic acid modifies transcriptional cytokine profile and induces early specific secretory IgA response in Giardia lamblia infected mice |
| title_sort | conjugated linoleic acid modifies transcriptional cytokine profile and induces early specific secretory iga response in giardia lamblia infected mice |
| topic | conjugated linoleic acid giardia lamblia il-10 secretory iga tgf-β |
| url | https://ijbms.mums.ac.ir/article_21158_a564f18bc37abfff0e0c5e0e38ff8b15.pdf |
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