Species Distribution And Antibiotic Susceptibility Of Nocardia Isolates From Yantai, China
Maoli Yi,* Lipeng Wang,* Weihao Xu, Li Sheng, Lihua Jiang, Fengzhen Yang, Qingmei Cao, Jinying Wu Department of Laboratory Medicine, Yantai Yuhuangding Hospital, Yantai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jinying WuDepartment Of Laborat...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-11-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/species-distribution-and-antibiotic-susceptibility-of-nocardia-isolate-peer-reviewed-article-IDR |
| _version_ | 1819153524807696384 |
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| author | Yi M Wang L Xu W Sheng L Jiang L Yang F Cao Q Wu J |
| author_facet | Yi M Wang L Xu W Sheng L Jiang L Yang F Cao Q Wu J |
| author_sort | Yi M |
| collection | DOAJ |
| description | Maoli Yi,* Lipeng Wang,* Weihao Xu, Li Sheng, Lihua Jiang, Fengzhen Yang, Qingmei Cao, Jinying Wu Department of Laboratory Medicine, Yantai Yuhuangding Hospital, Yantai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jinying WuDepartment Of Laboratory Medicine, Yantai Yuhuangding Hospital, 20# Yuhuangding East Road, Zhifu District, Yantai, Shandong Province 264000, People’s Republic of ChinaTel +86-0535-6691999-81505Email ymlytyhd@163.comPurpose: This study aimed to investigate the species distributions and drug sensitivities among 19 strains of Nocardia isolated from Yantai, China, from 2017 to 2019.Patients and methods: Definitive species identification was performed by sequencing a fragment of the 16S rRNA gene (1480 bp) and by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). The susceptibilities of the isolates to 15 commonly-used antibiotics were tested using the microbroth dilution method.Results: Among the 19 Nocardia isolates, five species were confirmed. Seventeen of the 19 Nocardia spp. strains were identified consistently by the two methods, while two isolates of N. cyriacigeorgica were misidentified as N. otitidiscaviarum by MALDI-TOF MS. N. farcinica was the most common species (8/19), followed by N. cyriacigeorgica (6/19), N. otitidiscaviarum (2/19), N. brasiliensis (2/19), and N. nova (1/19). All isolates were susceptible to trimethoprim-sulfamethoxazole and amikacin, followed by linezolid and tigecycline (94.7% susceptibility rates). The sensitivity and minimum inhibitory concentration patterns for ciprofloxacin, moxifloxacin, clarithromycin, and tobramycin were significantly correlated with the species.Conclusion: These results regarding the distribution and antibiotic resistance features of Nocardia species further our understanding of the diversity of Nocardia species circulating in Yantai, China, and thus support the use of more accurate empirical treatments.Keywords: antibiotic susceptibility, Nocardia, nocardiosis, species distribution
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| first_indexed | 2024-12-22T15:06:34Z |
| format | Article |
| id | doaj.art-f4986fa1e9904afda6df5651f0a6315b |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-22T15:06:34Z |
| publishDate | 2019-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-f4986fa1e9904afda6df5651f0a6315b2022-12-21T18:21:58ZengDove Medical PressInfection and Drug Resistance1178-69732019-11-01Volume 123653366149957Species Distribution And Antibiotic Susceptibility Of Nocardia Isolates From Yantai, ChinaYi MWang LXu WSheng LJiang LYang FCao QWu JMaoli Yi,* Lipeng Wang,* Weihao Xu, Li Sheng, Lihua Jiang, Fengzhen Yang, Qingmei Cao, Jinying Wu Department of Laboratory Medicine, Yantai Yuhuangding Hospital, Yantai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jinying WuDepartment Of Laboratory Medicine, Yantai Yuhuangding Hospital, 20# Yuhuangding East Road, Zhifu District, Yantai, Shandong Province 264000, People’s Republic of ChinaTel +86-0535-6691999-81505Email ymlytyhd@163.comPurpose: This study aimed to investigate the species distributions and drug sensitivities among 19 strains of Nocardia isolated from Yantai, China, from 2017 to 2019.Patients and methods: Definitive species identification was performed by sequencing a fragment of the 16S rRNA gene (1480 bp) and by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). The susceptibilities of the isolates to 15 commonly-used antibiotics were tested using the microbroth dilution method.Results: Among the 19 Nocardia isolates, five species were confirmed. Seventeen of the 19 Nocardia spp. strains were identified consistently by the two methods, while two isolates of N. cyriacigeorgica were misidentified as N. otitidiscaviarum by MALDI-TOF MS. N. farcinica was the most common species (8/19), followed by N. cyriacigeorgica (6/19), N. otitidiscaviarum (2/19), N. brasiliensis (2/19), and N. nova (1/19). All isolates were susceptible to trimethoprim-sulfamethoxazole and amikacin, followed by linezolid and tigecycline (94.7% susceptibility rates). The sensitivity and minimum inhibitory concentration patterns for ciprofloxacin, moxifloxacin, clarithromycin, and tobramycin were significantly correlated with the species.Conclusion: These results regarding the distribution and antibiotic resistance features of Nocardia species further our understanding of the diversity of Nocardia species circulating in Yantai, China, and thus support the use of more accurate empirical treatments.Keywords: antibiotic susceptibility, Nocardia, nocardiosis, species distribution https://www.dovepress.com/species-distribution-and-antibiotic-susceptibility-of-nocardia-isolate-peer-reviewed-article-IDRantibiotic susceptibilitynocardianocardiosisspecies distribution |
| spellingShingle | Yi M Wang L Xu W Sheng L Jiang L Yang F Cao Q Wu J Species Distribution And Antibiotic Susceptibility Of Nocardia Isolates From Yantai, China Infection and Drug Resistance antibiotic susceptibility nocardia nocardiosis species distribution |
| title | Species Distribution And Antibiotic Susceptibility Of Nocardia Isolates From Yantai, China |
| title_full | Species Distribution And Antibiotic Susceptibility Of Nocardia Isolates From Yantai, China |
| title_fullStr | Species Distribution And Antibiotic Susceptibility Of Nocardia Isolates From Yantai, China |
| title_full_unstemmed | Species Distribution And Antibiotic Susceptibility Of Nocardia Isolates From Yantai, China |
| title_short | Species Distribution And Antibiotic Susceptibility Of Nocardia Isolates From Yantai, China |
| title_sort | species distribution and antibiotic susceptibility of nocardia isolates from yantai china |
| topic | antibiotic susceptibility nocardia nocardiosis species distribution |
| url | https://www.dovepress.com/species-distribution-and-antibiotic-susceptibility-of-nocardia-isolate-peer-reviewed-article-IDR |
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