Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii.
Citral is a flavor component that is commonly used in food, beverage and fragrance industries. Cronobacter sakazakii is a food-borne pathogen associated with severe illness and high mortality in neonates and infants. The objective of the present study was to evaluate antimicrobial effect of citral a...
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| Format: | Article |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4945043?pdf=render |
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| author | Chao Shi Kaikuo Song Xiaorong Zhang Yi Sun Yue Sui Yifei Chen Zhenyu Jia Huihui Sun Zheng Sun Xiaodong Xia |
| author_facet | Chao Shi Kaikuo Song Xiaorong Zhang Yi Sun Yue Sui Yifei Chen Zhenyu Jia Huihui Sun Zheng Sun Xiaodong Xia |
| author_sort | Chao Shi |
| collection | DOAJ |
| description | Citral is a flavor component that is commonly used in food, beverage and fragrance industries. Cronobacter sakazakii is a food-borne pathogen associated with severe illness and high mortality in neonates and infants. The objective of the present study was to evaluate antimicrobial effect of citral against C. sakazakii strains. The minimum inhibitory concentration (MIC) of citral against C. sakazakii was determined via agar dilution method, then Gompertz models were used to quantitate the effect of citral on microbial growth kinetics. Changes in intracellular pH (pHin), membrane potential, intracellular ATP concentration, and membrane integrity were measured to elucidate the possible antimicrobial mechanism. Cell morphology changes were also examined using a field emission scanning electron microscope. The MICs of citral against C. sakazakii strains ranged from 0.27 to 0.54 mg/mL, and citral resulted in a longer lag phase and lower growth rate of C. sakazakii compared to the control. Citral affected the cell membrane of C. sakazakii, as evidenced by decreased intracellular ATP concentration, reduced pHin, and cell membrane hyperpolarization. Scanning electron microscopy analysis further confirmed that C. sakazakii cell membranes were damaged by citral. These findings suggest that citral exhibits antimicrobial effect against C. sakazakii strains and could be potentially used to control C. sakazakii in foods. However, how it works in food systems where many other components may interfere with its efficacy should be tested in future research before its real application. |
| first_indexed | 2024-12-23T23:04:28Z |
| format | Article |
| id | doaj.art-f49b195528a048e2a57bee41f9e2ec29 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T23:04:28Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f49b195528a048e2a57bee41f9e2ec292022-12-21T17:26:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01117e015900610.1371/journal.pone.0159006Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii.Chao ShiKaikuo SongXiaorong ZhangYi SunYue SuiYifei ChenZhenyu JiaHuihui SunZheng SunXiaodong XiaCitral is a flavor component that is commonly used in food, beverage and fragrance industries. Cronobacter sakazakii is a food-borne pathogen associated with severe illness and high mortality in neonates and infants. The objective of the present study was to evaluate antimicrobial effect of citral against C. sakazakii strains. The minimum inhibitory concentration (MIC) of citral against C. sakazakii was determined via agar dilution method, then Gompertz models were used to quantitate the effect of citral on microbial growth kinetics. Changes in intracellular pH (pHin), membrane potential, intracellular ATP concentration, and membrane integrity were measured to elucidate the possible antimicrobial mechanism. Cell morphology changes were also examined using a field emission scanning electron microscope. The MICs of citral against C. sakazakii strains ranged from 0.27 to 0.54 mg/mL, and citral resulted in a longer lag phase and lower growth rate of C. sakazakii compared to the control. Citral affected the cell membrane of C. sakazakii, as evidenced by decreased intracellular ATP concentration, reduced pHin, and cell membrane hyperpolarization. Scanning electron microscopy analysis further confirmed that C. sakazakii cell membranes were damaged by citral. These findings suggest that citral exhibits antimicrobial effect against C. sakazakii strains and could be potentially used to control C. sakazakii in foods. However, how it works in food systems where many other components may interfere with its efficacy should be tested in future research before its real application.http://europepmc.org/articles/PMC4945043?pdf=render |
| spellingShingle | Chao Shi Kaikuo Song Xiaorong Zhang Yi Sun Yue Sui Yifei Chen Zhenyu Jia Huihui Sun Zheng Sun Xiaodong Xia Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii. PLoS ONE |
| title | Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii. |
| title_full | Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii. |
| title_fullStr | Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii. |
| title_full_unstemmed | Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii. |
| title_short | Antimicrobial Activity and Possible Mechanism of Action of Citral against Cronobacter sakazakii. |
| title_sort | antimicrobial activity and possible mechanism of action of citral against cronobacter sakazakii |
| url | http://europepmc.org/articles/PMC4945043?pdf=render |
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