A Case of Bovine Eosinophilic Myositis (BEM) Associated with Co-Infection by <i>Sarcocystis hominis</i> and <i>Toxoplasma gondii</i>
Bovine eosinophilic myositis (BEM) is a specific inflammatory myopathy, often associated with <i>Sarcocystis</i> spp., with multifocal gray-green lesions leading to carcass condemnation with considerable economic losses. Here is described a peculiar case of BEM that occurred in an adult...
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MDPI AG
2023-01-01
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| Series: | Animals |
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| author | Filippo Maria Dini Monica Caffara Joana G. P. Jacinto Cinzia Benazzi Arcangelo Gentile Roberta Galuppi |
| author_facet | Filippo Maria Dini Monica Caffara Joana G. P. Jacinto Cinzia Benazzi Arcangelo Gentile Roberta Galuppi |
| author_sort | Filippo Maria Dini |
| collection | DOAJ |
| description | Bovine eosinophilic myositis (BEM) is a specific inflammatory myopathy, often associated with <i>Sarcocystis</i> spp., with multifocal gray-green lesions leading to carcass condemnation with considerable economic losses. Here is described a peculiar case of BEM that occurred in an adult (16 month) cattle, born in France, bred, and slaughtered in Italy at the end of 2021. On inspection, muscles showed the typical multifocal gray-green lesions that were sampled for, cytological, histological, and molecular investigations, while meat juice was subjected to IFAT for <i>Toxoplasma</i> IgG. Genomic DNA was extracted from lesions, portions of healthy muscle and from meat juice pellet and analyzed by PCR targeting 18S rDNA, COI mtDNA and B1 genes, and sequenced. The cytology showed inflammatory cells mostly referable to eosinophils; at histology, protozoan cysts and severe granulomatous myositis were observed. A BEM lesion and meat juice pellet subjected to PCR showed, concurrently, sequences referable both to <i>S. hominis</i> and <i>T. gondii</i>. Meat juice IFAT resulted negative for <i>T. gondii</i> IgG. Our findings highlight the first detection of <i>T. gondii</i> DNA in association with <i>S. hominis</i> in a BEM case, suggesting a multiple parasite infection associated with this pathology, although the actual role of <i>T. gondii</i> infection in the pathophysiology of the diseases should be clarified. |
| first_indexed | 2024-03-09T13:49:36Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T13:49:36Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-f4a75b831d4a445bab117cb594db13e32023-11-30T20:54:10ZengMDPI AGAnimals2076-26152023-01-0113231110.3390/ani13020311A Case of Bovine Eosinophilic Myositis (BEM) Associated with Co-Infection by <i>Sarcocystis hominis</i> and <i>Toxoplasma gondii</i>Filippo Maria Dini0Monica Caffara1Joana G. P. Jacinto2Cinzia Benazzi3Arcangelo Gentile4Roberta Galuppi5Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia, 40064 Bologna, ItalyBovine eosinophilic myositis (BEM) is a specific inflammatory myopathy, often associated with <i>Sarcocystis</i> spp., with multifocal gray-green lesions leading to carcass condemnation with considerable economic losses. Here is described a peculiar case of BEM that occurred in an adult (16 month) cattle, born in France, bred, and slaughtered in Italy at the end of 2021. On inspection, muscles showed the typical multifocal gray-green lesions that were sampled for, cytological, histological, and molecular investigations, while meat juice was subjected to IFAT for <i>Toxoplasma</i> IgG. Genomic DNA was extracted from lesions, portions of healthy muscle and from meat juice pellet and analyzed by PCR targeting 18S rDNA, COI mtDNA and B1 genes, and sequenced. The cytology showed inflammatory cells mostly referable to eosinophils; at histology, protozoan cysts and severe granulomatous myositis were observed. A BEM lesion and meat juice pellet subjected to PCR showed, concurrently, sequences referable both to <i>S. hominis</i> and <i>T. gondii</i>. Meat juice IFAT resulted negative for <i>T. gondii</i> IgG. Our findings highlight the first detection of <i>T. gondii</i> DNA in association with <i>S. hominis</i> in a BEM case, suggesting a multiple parasite infection associated with this pathology, although the actual role of <i>T. gondii</i> infection in the pathophysiology of the diseases should be clarified.https://www.mdpi.com/2076-2615/13/2/311<i>Sarcocystis hominis</i><i>Toxoplasma gondii</i>BEMcattlemeat-safetyApicomplexa |
| spellingShingle | Filippo Maria Dini Monica Caffara Joana G. P. Jacinto Cinzia Benazzi Arcangelo Gentile Roberta Galuppi A Case of Bovine Eosinophilic Myositis (BEM) Associated with Co-Infection by <i>Sarcocystis hominis</i> and <i>Toxoplasma gondii</i> Animals <i>Sarcocystis hominis</i> <i>Toxoplasma gondii</i> BEM cattle meat-safety Apicomplexa |
| title | A Case of Bovine Eosinophilic Myositis (BEM) Associated with Co-Infection by <i>Sarcocystis hominis</i> and <i>Toxoplasma gondii</i> |
| title_full | A Case of Bovine Eosinophilic Myositis (BEM) Associated with Co-Infection by <i>Sarcocystis hominis</i> and <i>Toxoplasma gondii</i> |
| title_fullStr | A Case of Bovine Eosinophilic Myositis (BEM) Associated with Co-Infection by <i>Sarcocystis hominis</i> and <i>Toxoplasma gondii</i> |
| title_full_unstemmed | A Case of Bovine Eosinophilic Myositis (BEM) Associated with Co-Infection by <i>Sarcocystis hominis</i> and <i>Toxoplasma gondii</i> |
| title_short | A Case of Bovine Eosinophilic Myositis (BEM) Associated with Co-Infection by <i>Sarcocystis hominis</i> and <i>Toxoplasma gondii</i> |
| title_sort | case of bovine eosinophilic myositis bem associated with co infection by i sarcocystis hominis i and i toxoplasma gondii i |
| topic | <i>Sarcocystis hominis</i> <i>Toxoplasma gondii</i> BEM cattle meat-safety Apicomplexa |
| url | https://www.mdpi.com/2076-2615/13/2/311 |
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