| Tóm tắt: | Eucalyptus globulus trees are available abundantly in the highland part of Ethiopia and traditionally used for the treatment of various ailments including influenza, common cold, warts, febrile illness, and headache. In view of its traditional uses, an attempt was made to explore the chemical profile and antibacterial studies of the leaves of Eucalyptus globulus from two district area. The chemical composition of essential oil obtained by steam distillation extraction from the leaves of Eucalyptus globulus was analyzed by GC–MS. The extraction yields of essential oils of the leaves of E. globulus collected from Haramaya Campus, and Entoto Park were 0.23% and 0.24%, respectively. Twenty-one compounds, constituting about 99.20% of the total oil, were identified from the Haramaya University Campus, whereas eighteen compounds, constituting about 98.03% of the total oil, were identified from Entoto Park. The main constituents identified in both areas were 1, 8-cineole (68.25-72.10%), cis-sabinol (12.10-14.31%), Limonene (2.35-2.96%), α-pinene (2.15-2.50%) and α-terpineol (1.62-2.13. The antimicrobial activity of the oil was evaluated against four microorganisms using paper disk diffusion methods. Essential oils extract of Eucalyptus globulus leaves from Haramaya Campus showed comparatively less antibacterial activity than Entoto Park.
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