Integrative Phytohormone and Transcriptome Analyses Reveal the Inhibitory Mechanism of Ethylene on Potato Tuber Sprouting at Room Temperature
As a commercial potato sprout suppressant, ethylene (Eth) is usually used under a low temperature for long-term storage of potato tubers. However, in many cases, potato tubers are usually transferred from a refrigeration house and sold at room temperature. In the present research, Eth’s inhibitory e...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/3/286 |
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| author | Meixue Zhang Wenxiao Jiao Qingmin Chen Maorun Fu Cong Han |
| author_facet | Meixue Zhang Wenxiao Jiao Qingmin Chen Maorun Fu Cong Han |
| author_sort | Meixue Zhang |
| collection | DOAJ |
| description | As a commercial potato sprout suppressant, ethylene (Eth) is usually used under a low temperature for long-term storage of potato tubers. However, in many cases, potato tubers are usually transferred from a refrigeration house and sold at room temperature. In the present research, Eth’s inhibitory effects on tuber sprouting at room temperature were investigated. The potential molecular mechanisms of Eth-induced sprout suppression were revealed by phytohormone and transcriptome analyses. Results showed that exogenous Eth significantly suppressed sprout growth in potato tubers during two weeks of storage. The endogenous plant hormone levels of abscisic acid (ABA) and auxin (Aux) were markedly reduced by Eth treatment. Transcriptomic analysis revealed that more transcriptional changes occurred in the early stage of sprouting. The differentially expressed genes (DEGs) assigned to the pathways of plant hormone signal transduction, photosynthesis, starch and sucrose metabolism and phenylpropanoid biosynthesis, which were closely associated with sprouting, were extensively down-regulated by Eth treatment. In addition, the heat map of six hormone signal transduction pathways showed that the expression of most DEGs annotated into the Aux signal transduction pathway was suppressed by Eth treatment, while the expression of many DEGs annotated as <i>ETR</i> (Eth receptor) and <i>ERF1/2</i> (Eth-responsive transcription factor 1 and 2) in the Eth signal transduction pathway was enhanced by Eth treatment. Taken together, our results indicated that Eth-induced sprout inhibition might be closely related to the suppression of internal Aux production and signal transduction and the activation of Eth signal transduction. |
| first_indexed | 2024-04-24T18:14:07Z |
| format | Article |
| id | doaj.art-f4a83c35a34c46bf854b253e6e15cc8b |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-04-24T18:14:07Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-f4a83c35a34c46bf854b253e6e15cc8b2024-03-27T13:44:29ZengMDPI AGHorticulturae2311-75242024-03-0110328610.3390/horticulturae10030286Integrative Phytohormone and Transcriptome Analyses Reveal the Inhibitory Mechanism of Ethylene on Potato Tuber Sprouting at Room TemperatureMeixue Zhang0Wenxiao Jiao1Qingmin Chen2Maorun Fu3Cong Han4College of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaCollege of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaCollege of Food Science and Engineering, Shandong Agricultural and Engineering University, Jinan 250100, ChinaCollege of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaCollege of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, ChinaAs a commercial potato sprout suppressant, ethylene (Eth) is usually used under a low temperature for long-term storage of potato tubers. However, in many cases, potato tubers are usually transferred from a refrigeration house and sold at room temperature. In the present research, Eth’s inhibitory effects on tuber sprouting at room temperature were investigated. The potential molecular mechanisms of Eth-induced sprout suppression were revealed by phytohormone and transcriptome analyses. Results showed that exogenous Eth significantly suppressed sprout growth in potato tubers during two weeks of storage. The endogenous plant hormone levels of abscisic acid (ABA) and auxin (Aux) were markedly reduced by Eth treatment. Transcriptomic analysis revealed that more transcriptional changes occurred in the early stage of sprouting. The differentially expressed genes (DEGs) assigned to the pathways of plant hormone signal transduction, photosynthesis, starch and sucrose metabolism and phenylpropanoid biosynthesis, which were closely associated with sprouting, were extensively down-regulated by Eth treatment. In addition, the heat map of six hormone signal transduction pathways showed that the expression of most DEGs annotated into the Aux signal transduction pathway was suppressed by Eth treatment, while the expression of many DEGs annotated as <i>ETR</i> (Eth receptor) and <i>ERF1/2</i> (Eth-responsive transcription factor 1 and 2) in the Eth signal transduction pathway was enhanced by Eth treatment. Taken together, our results indicated that Eth-induced sprout inhibition might be closely related to the suppression of internal Aux production and signal transduction and the activation of Eth signal transduction.https://www.mdpi.com/2311-7524/10/3/286potatosproutingRNA-seqauxinsignal transduction |
| spellingShingle | Meixue Zhang Wenxiao Jiao Qingmin Chen Maorun Fu Cong Han Integrative Phytohormone and Transcriptome Analyses Reveal the Inhibitory Mechanism of Ethylene on Potato Tuber Sprouting at Room Temperature Horticulturae potato sprouting RNA-seq auxin signal transduction |
| title | Integrative Phytohormone and Transcriptome Analyses Reveal the Inhibitory Mechanism of Ethylene on Potato Tuber Sprouting at Room Temperature |
| title_full | Integrative Phytohormone and Transcriptome Analyses Reveal the Inhibitory Mechanism of Ethylene on Potato Tuber Sprouting at Room Temperature |
| title_fullStr | Integrative Phytohormone and Transcriptome Analyses Reveal the Inhibitory Mechanism of Ethylene on Potato Tuber Sprouting at Room Temperature |
| title_full_unstemmed | Integrative Phytohormone and Transcriptome Analyses Reveal the Inhibitory Mechanism of Ethylene on Potato Tuber Sprouting at Room Temperature |
| title_short | Integrative Phytohormone and Transcriptome Analyses Reveal the Inhibitory Mechanism of Ethylene on Potato Tuber Sprouting at Room Temperature |
| title_sort | integrative phytohormone and transcriptome analyses reveal the inhibitory mechanism of ethylene on potato tuber sprouting at room temperature |
| topic | potato sprouting RNA-seq auxin signal transduction |
| url | https://www.mdpi.com/2311-7524/10/3/286 |
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