Effects of <i>Fusarium proliferatum</i> on Aboveground Physiological Indicators of Superior Apple Rootstock Line 12-2 (<i>Malus spectabilis</i>) with Improved Apple-Replant-Disease Resistance
(1) Background: Cultivating resistant rootstocks is an effective way to mitigate apple replant disease (ARD), and we developed superior apple rootstock line 12-2 (self-named), which shows improved ARD resistance. (2) Methods: We used ARD-associated pathogen <i>Fusarium proliferatum</i> M...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/8/723 |
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| author | Xueli Cui Xin Qin Yangbo Liu Yanli Hu Zhiquan Mao Xuesen Chen Yunfei Mao Xiang Shen |
| author_facet | Xueli Cui Xin Qin Yangbo Liu Yanli Hu Zhiquan Mao Xuesen Chen Yunfei Mao Xiang Shen |
| author_sort | Xueli Cui |
| collection | DOAJ |
| description | (1) Background: Cultivating resistant rootstocks is an effective way to mitigate apple replant disease (ARD), and we developed superior apple rootstock line 12-2 (self-named), which shows improved ARD resistance. (2) Methods: We used ARD-associated pathogen <i>Fusarium proliferatum</i> MR5 (MR5) to test the fungal infection in the 12-2 line. Seedlings of the 12-2, T337, and M26 rootstock lines were planted in a substrate with potato dextrose broth and MR5 spore solution, and aboveground physiological indicators were measured. (3) Results: MR5 had the greatest effect on the leaf growth of T337 and M26. The incidence rates of infectious symptoms in the T337 and M26 lines were 68 and 100%, respectively. MR5 significantly affected the leaf chlorophyll content, ETR, and NPQ of T337 and M26, as well as <i>P</i><sub>n</sub> and <i>T</i><sub>r</sub> of M26. MR5 tended to reduce the leaf photosynthetic parameters of T337, but the decreases were not significant. The leaf reactive-oxygen-species levels of T337 and M26, the leaf antioxidant-enzyme activities of M26, and the superoxide-dismutase activity of T337 were significantly affected by MR5. MR5 also had a significant effect on the leaf <i>malondialdehyde</i>, proline, and soluble-sugar contents of T337 and M26. None of these aboveground physiological indicators were affected by MR5 in the 12-2 rootstock. (4) Conclusions: The 12-2 rootstock was more resistant to ARD-associated MR5 and could serve as an important test material for resistant-apple-rootstock breeding in China. |
| first_indexed | 2024-03-09T13:19:58Z |
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| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T13:19:58Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-f4ac55f01691444080b4f25743a42c112023-11-30T21:31:17ZengMDPI AGHorticulturae2311-75242022-08-018872310.3390/horticulturae8080723Effects of <i>Fusarium proliferatum</i> on Aboveground Physiological Indicators of Superior Apple Rootstock Line 12-2 (<i>Malus spectabilis</i>) with Improved Apple-Replant-Disease ResistanceXueli Cui0Xin Qin1Yangbo Liu2Yanli Hu3Zhiquan Mao4Xuesen Chen5Yunfei Mao6Xiang Shen7National Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaNational Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaNational Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaNational Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaNational Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaNational Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaNational Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaNational Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, China(1) Background: Cultivating resistant rootstocks is an effective way to mitigate apple replant disease (ARD), and we developed superior apple rootstock line 12-2 (self-named), which shows improved ARD resistance. (2) Methods: We used ARD-associated pathogen <i>Fusarium proliferatum</i> MR5 (MR5) to test the fungal infection in the 12-2 line. Seedlings of the 12-2, T337, and M26 rootstock lines were planted in a substrate with potato dextrose broth and MR5 spore solution, and aboveground physiological indicators were measured. (3) Results: MR5 had the greatest effect on the leaf growth of T337 and M26. The incidence rates of infectious symptoms in the T337 and M26 lines were 68 and 100%, respectively. MR5 significantly affected the leaf chlorophyll content, ETR, and NPQ of T337 and M26, as well as <i>P</i><sub>n</sub> and <i>T</i><sub>r</sub> of M26. MR5 tended to reduce the leaf photosynthetic parameters of T337, but the decreases were not significant. The leaf reactive-oxygen-species levels of T337 and M26, the leaf antioxidant-enzyme activities of M26, and the superoxide-dismutase activity of T337 were significantly affected by MR5. MR5 also had a significant effect on the leaf <i>malondialdehyde</i>, proline, and soluble-sugar contents of T337 and M26. None of these aboveground physiological indicators were affected by MR5 in the 12-2 rootstock. (4) Conclusions: The 12-2 rootstock was more resistant to ARD-associated MR5 and could serve as an important test material for resistant-apple-rootstock breeding in China.https://www.mdpi.com/2311-7524/8/8/723cultivation of resistant rootstocksARDARD-associated <i>F. proliferatum</i> MR5apple rootstock superior line 12-2aboveground physiological indicators |
| spellingShingle | Xueli Cui Xin Qin Yangbo Liu Yanli Hu Zhiquan Mao Xuesen Chen Yunfei Mao Xiang Shen Effects of <i>Fusarium proliferatum</i> on Aboveground Physiological Indicators of Superior Apple Rootstock Line 12-2 (<i>Malus spectabilis</i>) with Improved Apple-Replant-Disease Resistance Horticulturae cultivation of resistant rootstocks ARD ARD-associated <i>F. proliferatum</i> MR5 apple rootstock superior line 12-2 aboveground physiological indicators |
| title | Effects of <i>Fusarium proliferatum</i> on Aboveground Physiological Indicators of Superior Apple Rootstock Line 12-2 (<i>Malus spectabilis</i>) with Improved Apple-Replant-Disease Resistance |
| title_full | Effects of <i>Fusarium proliferatum</i> on Aboveground Physiological Indicators of Superior Apple Rootstock Line 12-2 (<i>Malus spectabilis</i>) with Improved Apple-Replant-Disease Resistance |
| title_fullStr | Effects of <i>Fusarium proliferatum</i> on Aboveground Physiological Indicators of Superior Apple Rootstock Line 12-2 (<i>Malus spectabilis</i>) with Improved Apple-Replant-Disease Resistance |
| title_full_unstemmed | Effects of <i>Fusarium proliferatum</i> on Aboveground Physiological Indicators of Superior Apple Rootstock Line 12-2 (<i>Malus spectabilis</i>) with Improved Apple-Replant-Disease Resistance |
| title_short | Effects of <i>Fusarium proliferatum</i> on Aboveground Physiological Indicators of Superior Apple Rootstock Line 12-2 (<i>Malus spectabilis</i>) with Improved Apple-Replant-Disease Resistance |
| title_sort | effects of i fusarium proliferatum i on aboveground physiological indicators of superior apple rootstock line 12 2 i malus spectabilis i with improved apple replant disease resistance |
| topic | cultivation of resistant rootstocks ARD ARD-associated <i>F. proliferatum</i> MR5 apple rootstock superior line 12-2 aboveground physiological indicators |
| url | https://www.mdpi.com/2311-7524/8/8/723 |
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