The Effect of Gut Microbiota on the Progression of Intervertebral Disc Degeneration
Objective Intervertebral disc degeneration (IDD) is the main cause of back pain, and its treatment is a serious socio‐economic burden. The safety and treatment of fecal microbiota transplantation (FMT) has been established. However, the relationship between FMT and IDD still unclear. We aimed to exp...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | Orthopaedic Surgery |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/os.13626 |
| _version_ | 1811162050903146496 |
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| author | Bo Yao Youquan Cai Weiguo Wang Jia Deng Lei Zhao Ziwei Han Li Wan |
| author_facet | Bo Yao Youquan Cai Weiguo Wang Jia Deng Lei Zhao Ziwei Han Li Wan |
| author_sort | Bo Yao |
| collection | DOAJ |
| description | Objective Intervertebral disc degeneration (IDD) is the main cause of back pain, and its treatment is a serious socio‐economic burden. The safety and treatment of fecal microbiota transplantation (FMT) has been established. However, the relationship between FMT and IDD still unclear. We aimed to explore whether FMT plays a role in IDD to provide a reference for the treatment of IDD. Methods An experimental model of IDD was established using 2‐month‐old male Sprague–Dawley rats. FMT was performed by intragastric gavage of IDD rats with a fecal bacterial solution. Rat serum, feces, and vertebral disc tissue were collected after surgery for 2 months. The levels of TNF‐α, IL‐1β, IL‐6, matrix metalloproteinase (MMP)‐3, MMP‐13, Collagen II, and aggrecan in the serum or vertebral disc tissue were measured by an enzyme‐linked immunosorbent assay, immunohistochemistry, quantitative real‐time polymerase chain reaction, or western blotting. We also examined the pathology of the vertebral disc tissue using hematoxylin and eosin (HE) and safranin O‐fast green staining. Finally, we examined the gut microbiota in rat feces using 16 S rRNA gene sequencing. Results We found that the expression of TNF‐α, IL‐1β, IL‐6, MMP‐3, MMP‐13, NLRP3 and Caspase‐1 increased in the IDD group rats. In contrast, Collagen II and aggrecan levels were downregulated. Additionally, vertebral disc tissue was severely damaged in the IDD group, with disordered cell arrangement and uneven safranin coloration. FMT reversed the effects of IDD modeling on these factors and alleviated cartilage tissue damage. In addition, FMT increased the gut microbiota diversity and microbial abundance in rats treated with IDD. Conclusion Our findings suggest that FMT has a positive effect in maintaining cellular stability in the vertebral disc and alleviating histopathological damage. It affects the diversity and abundance of gut microbiota in rats with IDD. Therefore, FMT may serve as a promising target for amelioration of IDD. |
| first_indexed | 2024-04-10T06:23:22Z |
| format | Article |
| id | doaj.art-f4acf35278c543498949cb7125243c72 |
| institution | Directory Open Access Journal |
| issn | 1757-7853 1757-7861 |
| language | English |
| last_indexed | 2024-04-10T06:23:22Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Orthopaedic Surgery |
| spelling | doaj.art-f4acf35278c543498949cb7125243c722023-03-02T00:52:51ZengWileyOrthopaedic Surgery1757-78531757-78612023-03-0115385886710.1111/os.13626The Effect of Gut Microbiota on the Progression of Intervertebral Disc DegenerationBo Yao0Youquan Cai1Weiguo Wang2Jia Deng3Lei Zhao4Ziwei Han5Li Wan6Department of Spine Surgery Want want hospital Changsha Hunan ChinaDepartment of Spine Surgery Want want hospital Changsha Hunan ChinaDepartment of Spine Surgery, the Third Xiangya Hospital Central South University Changsha Hunan ChinaDepartment of Spine Surgery Want want hospital Changsha Hunan ChinaDepartment of Spine Surgery Want want hospital Changsha Hunan ChinaDepartment of Spine Surgery Want want hospital Changsha Hunan ChinaDepartment of Spine Surgery Want want hospital Changsha Hunan ChinaObjective Intervertebral disc degeneration (IDD) is the main cause of back pain, and its treatment is a serious socio‐economic burden. The safety and treatment of fecal microbiota transplantation (FMT) has been established. However, the relationship between FMT and IDD still unclear. We aimed to explore whether FMT plays a role in IDD to provide a reference for the treatment of IDD. Methods An experimental model of IDD was established using 2‐month‐old male Sprague–Dawley rats. FMT was performed by intragastric gavage of IDD rats with a fecal bacterial solution. Rat serum, feces, and vertebral disc tissue were collected after surgery for 2 months. The levels of TNF‐α, IL‐1β, IL‐6, matrix metalloproteinase (MMP)‐3, MMP‐13, Collagen II, and aggrecan in the serum or vertebral disc tissue were measured by an enzyme‐linked immunosorbent assay, immunohistochemistry, quantitative real‐time polymerase chain reaction, or western blotting. We also examined the pathology of the vertebral disc tissue using hematoxylin and eosin (HE) and safranin O‐fast green staining. Finally, we examined the gut microbiota in rat feces using 16 S rRNA gene sequencing. Results We found that the expression of TNF‐α, IL‐1β, IL‐6, MMP‐3, MMP‐13, NLRP3 and Caspase‐1 increased in the IDD group rats. In contrast, Collagen II and aggrecan levels were downregulated. Additionally, vertebral disc tissue was severely damaged in the IDD group, with disordered cell arrangement and uneven safranin coloration. FMT reversed the effects of IDD modeling on these factors and alleviated cartilage tissue damage. In addition, FMT increased the gut microbiota diversity and microbial abundance in rats treated with IDD. Conclusion Our findings suggest that FMT has a positive effect in maintaining cellular stability in the vertebral disc and alleviating histopathological damage. It affects the diversity and abundance of gut microbiota in rats with IDD. Therefore, FMT may serve as a promising target for amelioration of IDD.https://doi.org/10.1111/os.13626AutophagyFecal Microbiota TransplantationGut MicrobiotaInflammationIntervertebral Disc Degeneration |
| spellingShingle | Bo Yao Youquan Cai Weiguo Wang Jia Deng Lei Zhao Ziwei Han Li Wan The Effect of Gut Microbiota on the Progression of Intervertebral Disc Degeneration Orthopaedic Surgery Autophagy Fecal Microbiota Transplantation Gut Microbiota Inflammation Intervertebral Disc Degeneration |
| title | The Effect of Gut Microbiota on the Progression of Intervertebral Disc Degeneration |
| title_full | The Effect of Gut Microbiota on the Progression of Intervertebral Disc Degeneration |
| title_fullStr | The Effect of Gut Microbiota on the Progression of Intervertebral Disc Degeneration |
| title_full_unstemmed | The Effect of Gut Microbiota on the Progression of Intervertebral Disc Degeneration |
| title_short | The Effect of Gut Microbiota on the Progression of Intervertebral Disc Degeneration |
| title_sort | effect of gut microbiota on the progression of intervertebral disc degeneration |
| topic | Autophagy Fecal Microbiota Transplantation Gut Microbiota Inflammation Intervertebral Disc Degeneration |
| url | https://doi.org/10.1111/os.13626 |
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