Evaluation of sensitivity and specificity of CanPatrol™ technology for detection of circulating tumor cells in patients with non-small cell lung cancer
Abstract Background The early diagnosis of non-small cell lung cancer is of great significance to the prognosis of patients. However, traditional histopathology and imaging screening have certain limitations. Therefore, new diagnostical methods are urgently needed for the current clinical diagnosis....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-10-01
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| Series: | BMC Pulmonary Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12890-020-01314-4 |
| _version_ | 1818291566349910016 |
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| author | Jingyao Li Yi Liao Yaling Ran Guiyu Wang Wei Wu Yang Qiu Jie Liu Ningyu Wen Tao Jing Haidong Wang Shixin Zhang |
| author_facet | Jingyao Li Yi Liao Yaling Ran Guiyu Wang Wei Wu Yang Qiu Jie Liu Ningyu Wen Tao Jing Haidong Wang Shixin Zhang |
| author_sort | Jingyao Li |
| collection | DOAJ |
| description | Abstract Background The early diagnosis of non-small cell lung cancer is of great significance to the prognosis of patients. However, traditional histopathology and imaging screening have certain limitations. Therefore, new diagnostical methods are urgently needed for the current clinical diagnosis. In this study we evaluated the sensitivity and specificity of CanPatrol™ technology for the detection of circulating tumor cells in patients with non-small cell lung cancer (NSCLC). Methods CTCs in the peripheral blood of 98 patients with NSCLC and 38 patients with benign pulmonary diseases were collected by the latest typing of CanPatrol™ detection technology. A 3-year follow-up was performed to observe their recurrence and metastasis. Kruskal-Wallis test was used to compare multiple groups of data, Mann-Whitney U test was used to compare data between the two groups, and ROC curve analysis was used to obtain the critical value. The COX risk regression and Kaplan-Meier survival analysis were performed in the 63 NSCLC patients who were effectively followed up. Results The epithelial, epithelial-mesenchymal, and total CTCs were significantly higher in NSCLC patients than that in patients with benign lung disease (P < 0.001). The mesenchymal CTCs of NSCLC patients was slightly higher than that of benign lung diseases (P = 0.013). The AUC of the ROC curve of the total CTCs was 0.837 (95% CI: 0.76-0.914), and the cut-off value corresponding to the most approximate index was 0.5 CTCs/5 ml, at which point the sensitivity was 81.6% and the specificity was 86.8%. COX regression analysis revealed that the clinical stage was correlated with patient survival (P = 0.006), while gender, age, and smoking were not (P > 0.05). After excluding the confounders of staging, surgery, and chemotherapy, Kaplan-Meier survival analysis showed that patients in stage IIIA with CTCs ≥0.5 had significantly lower DFS than those with CTCs < 0.5 (P = 0.022). Conclusions CTC positive can well predict the recurrence of NSCLC patients. CanPatrol™ technology has good sensitivity and specificity in detecting CTCs in peripheral blood of NSCLC patients and has a certain value for clinical prognosis evaluation. |
| first_indexed | 2024-12-13T02:46:06Z |
| format | Article |
| id | doaj.art-f4ad5159775346d5b65ecab8bf55fcd0 |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-12-13T02:46:06Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-f4ad5159775346d5b65ecab8bf55fcd02022-12-22T00:02:12ZengBMCBMC Pulmonary Medicine1471-24662020-10-012011910.1186/s12890-020-01314-4Evaluation of sensitivity and specificity of CanPatrol™ technology for detection of circulating tumor cells in patients with non-small cell lung cancerJingyao Li0Yi Liao1Yaling Ran2Guiyu Wang3Wei Wu4Yang Qiu5Jie Liu6Ningyu Wen7Tao Jing8Haidong Wang9Shixin Zhang10Department of Thoracic Surgery, Southwest Hospital, Army Medical University (Third Military Medical University)Department of Thoracic Surgery, Southwest Hospital, Army Medical University (Third Military Medical University)SurExam Bio-Tech, Guangzhou Technology Innovation BaseDepartment of Clinical Laboratory, Center of Laboratory Medical, Southwest Hospital, Army Medical University (Third Military Medical University)Department of Thoracic Surgery, Southwest Hospital, Army Medical University (Third Military Medical University)Department of Thoracic Surgery, Southwest Hospital, Army Medical University (Third Military Medical University)Department of Thoracic Surgery, Southwest Hospital, Army Medical University (Third Military Medical University)Department of Thoracic Surgery, Southwest Hospital, Army Medical University (Third Military Medical University)Department of Vasculocardiology, Southwest Hospital, Army Medical University (Third Military Medical University)Department of Thoracic Surgery, Southwest Hospital, Army Medical University (Third Military Medical University)Department of Thoracic Surgery, Southwest Hospital, Army Medical University (Third Military Medical University)Abstract Background The early diagnosis of non-small cell lung cancer is of great significance to the prognosis of patients. However, traditional histopathology and imaging screening have certain limitations. Therefore, new diagnostical methods are urgently needed for the current clinical diagnosis. In this study we evaluated the sensitivity and specificity of CanPatrol™ technology for the detection of circulating tumor cells in patients with non-small cell lung cancer (NSCLC). Methods CTCs in the peripheral blood of 98 patients with NSCLC and 38 patients with benign pulmonary diseases were collected by the latest typing of CanPatrol™ detection technology. A 3-year follow-up was performed to observe their recurrence and metastasis. Kruskal-Wallis test was used to compare multiple groups of data, Mann-Whitney U test was used to compare data between the two groups, and ROC curve analysis was used to obtain the critical value. The COX risk regression and Kaplan-Meier survival analysis were performed in the 63 NSCLC patients who were effectively followed up. Results The epithelial, epithelial-mesenchymal, and total CTCs were significantly higher in NSCLC patients than that in patients with benign lung disease (P < 0.001). The mesenchymal CTCs of NSCLC patients was slightly higher than that of benign lung diseases (P = 0.013). The AUC of the ROC curve of the total CTCs was 0.837 (95% CI: 0.76-0.914), and the cut-off value corresponding to the most approximate index was 0.5 CTCs/5 ml, at which point the sensitivity was 81.6% and the specificity was 86.8%. COX regression analysis revealed that the clinical stage was correlated with patient survival (P = 0.006), while gender, age, and smoking were not (P > 0.05). After excluding the confounders of staging, surgery, and chemotherapy, Kaplan-Meier survival analysis showed that patients in stage IIIA with CTCs ≥0.5 had significantly lower DFS than those with CTCs < 0.5 (P = 0.022). Conclusions CTC positive can well predict the recurrence of NSCLC patients. CanPatrol™ technology has good sensitivity and specificity in detecting CTCs in peripheral blood of NSCLC patients and has a certain value for clinical prognosis evaluation.http://link.springer.com/article/10.1186/s12890-020-01314-4NSCLCCTCsCanPatrol™SensitivitySpecificity |
| spellingShingle | Jingyao Li Yi Liao Yaling Ran Guiyu Wang Wei Wu Yang Qiu Jie Liu Ningyu Wen Tao Jing Haidong Wang Shixin Zhang Evaluation of sensitivity and specificity of CanPatrol™ technology for detection of circulating tumor cells in patients with non-small cell lung cancer BMC Pulmonary Medicine NSCLC CTCs CanPatrol™ Sensitivity Specificity |
| title | Evaluation of sensitivity and specificity of CanPatrol™ technology for detection of circulating tumor cells in patients with non-small cell lung cancer |
| title_full | Evaluation of sensitivity and specificity of CanPatrol™ technology for detection of circulating tumor cells in patients with non-small cell lung cancer |
| title_fullStr | Evaluation of sensitivity and specificity of CanPatrol™ technology for detection of circulating tumor cells in patients with non-small cell lung cancer |
| title_full_unstemmed | Evaluation of sensitivity and specificity of CanPatrol™ technology for detection of circulating tumor cells in patients with non-small cell lung cancer |
| title_short | Evaluation of sensitivity and specificity of CanPatrol™ technology for detection of circulating tumor cells in patients with non-small cell lung cancer |
| title_sort | evaluation of sensitivity and specificity of canpatrol™ technology for detection of circulating tumor cells in patients with non small cell lung cancer |
| topic | NSCLC CTCs CanPatrol™ Sensitivity Specificity |
| url | http://link.springer.com/article/10.1186/s12890-020-01314-4 |
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