The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein 2 of <i>Euplatypus parallelus</i> to Semiochemicals
<i>Euplatypus parallelus</i> is one of the dominant rubber bark beetle species in Hainan’s rubber-planting area. Semiochemicals, including the volatiles found in rubber trees and aggregation pheromones, play an important role in the search for suitable host plants. To examine the possibl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/2/1714 |
| _version_ | 1797441119653462016 |
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| author | Guangchao Cui Xiang Zhou Qian Wang Kai Zhang Lei Qin Jixing Guo |
| author_facet | Guangchao Cui Xiang Zhou Qian Wang Kai Zhang Lei Qin Jixing Guo |
| author_sort | Guangchao Cui |
| collection | DOAJ |
| description | <i>Euplatypus parallelus</i> is one of the dominant rubber bark beetle species in Hainan’s rubber-planting area. Semiochemicals, including the volatiles found in rubber trees and aggregation pheromones, play an important role in the search for suitable host plants. To examine the possible functional role of highly expressed odorant-binding protein 2 of <i>Euplatypus parallelus</i> (EparOBP2) in the semiochemical recognition process, we cloned and analyzed the cDNA sequence of EparOBP2. The results showed that EparOBP2 contains an open reading frame (ORF) of 393 bp that encodes 130 amino acids, including a 21-amino-acid residue signal peptide at the N-terminus. The matured EparOBP2 protein consists of seven α-helices, creating an open binding pocket and three disulfide bridges. The results of the fluorescence binding assay showed that EparOBP2 had high binding ability with α-pinene and myrcene. The docking results confirmed that the interactions of α-pinene and myrcene with EparOBP2 were primarily achieved through hydrophobic interactions. This study provides evidence that EparOBP2 may be involved in the chemoreception of semiochemicals and that it can successfully contribute to the integrated management of <i>E. parallelus</i>. |
| first_indexed | 2024-03-09T12:18:25Z |
| format | Article |
| id | doaj.art-f4ae329eb3b44d1fbec12f2e2bd0b368 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:18:25Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f4ae329eb3b44d1fbec12f2e2bd0b3682023-11-30T22:44:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242171410.3390/ijms24021714The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein 2 of <i>Euplatypus parallelus</i> to SemiochemicalsGuangchao Cui0Xiang Zhou1Qian Wang2Kai Zhang3Lei Qin4Jixing Guo5Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Plant Protection, Hainan University, Haikou 570228, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Plant Protection, Hainan University, Haikou 570228, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Plant Protection, Hainan University, Haikou 570228, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Plant Protection, Hainan University, Haikou 570228, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Plant Protection, Hainan University, Haikou 570228, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Plant Protection, Hainan University, Haikou 570228, China<i>Euplatypus parallelus</i> is one of the dominant rubber bark beetle species in Hainan’s rubber-planting area. Semiochemicals, including the volatiles found in rubber trees and aggregation pheromones, play an important role in the search for suitable host plants. To examine the possible functional role of highly expressed odorant-binding protein 2 of <i>Euplatypus parallelus</i> (EparOBP2) in the semiochemical recognition process, we cloned and analyzed the cDNA sequence of EparOBP2. The results showed that EparOBP2 contains an open reading frame (ORF) of 393 bp that encodes 130 amino acids, including a 21-amino-acid residue signal peptide at the N-terminus. The matured EparOBP2 protein consists of seven α-helices, creating an open binding pocket and three disulfide bridges. The results of the fluorescence binding assay showed that EparOBP2 had high binding ability with α-pinene and myrcene. The docking results confirmed that the interactions of α-pinene and myrcene with EparOBP2 were primarily achieved through hydrophobic interactions. This study provides evidence that EparOBP2 may be involved in the chemoreception of semiochemicals and that it can successfully contribute to the integrated management of <i>E. parallelus</i>.https://www.mdpi.com/1422-0067/24/2/1714odorant-binding proteins<i>Euplatypus parallelus</i>binding abilityfluorescence competitive binding assaysmolecular docking |
| spellingShingle | Guangchao Cui Xiang Zhou Qian Wang Kai Zhang Lei Qin Jixing Guo The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein 2 of <i>Euplatypus parallelus</i> to Semiochemicals International Journal of Molecular Sciences odorant-binding proteins <i>Euplatypus parallelus</i> binding ability fluorescence competitive binding assays molecular docking |
| title | The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein 2 of <i>Euplatypus parallelus</i> to Semiochemicals |
| title_full | The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein 2 of <i>Euplatypus parallelus</i> to Semiochemicals |
| title_fullStr | The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein 2 of <i>Euplatypus parallelus</i> to Semiochemicals |
| title_full_unstemmed | The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein 2 of <i>Euplatypus parallelus</i> to Semiochemicals |
| title_short | The Sequence Characteristics and Binding Properties of the Odorant-Binding Protein 2 of <i>Euplatypus parallelus</i> to Semiochemicals |
| title_sort | sequence characteristics and binding properties of the odorant binding protein 2 of i euplatypus parallelus i to semiochemicals |
| topic | odorant-binding proteins <i>Euplatypus parallelus</i> binding ability fluorescence competitive binding assays molecular docking |
| url | https://www.mdpi.com/1422-0067/24/2/1714 |
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