Evaluation and comparison of bioactive substances in selected species of the genus Allium

<!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:RelyOnVML /> <o:AllowPNG /> </o:OfficeDocumentSettings> </xml><![endif]--> <p class="PotText" style="line-height: 115%;"><em>Allium</em> is a genus of some 650...

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Main Authors: Ján Kovarovič, Judita Bystrická, Alexander Fehér, Marianna Lenková
Format: Article
Language:English
Published: HACCP Consulting 2017-01-01
Series:Potravinarstvo
Subjects:
Online Access:http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/833
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author Ján Kovarovič
Judita Bystrická
Alexander Fehér
Marianna Lenková
author_facet Ján Kovarovič
Judita Bystrická
Alexander Fehér
Marianna Lenková
author_sort Ján Kovarovič
collection DOAJ
description <!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:RelyOnVML /> <o:AllowPNG /> </o:OfficeDocumentSettings> </xml><![endif]--> <p class="PotText" style="line-height: 115%;"><em>Allium</em> is a genus of some 650 species belonging to the family <em>Liliaceae</em>. However only a few of these are important as food plants, notably garlic (<em>Allium sativum</em> L.), onion (<em>Allium cepa</em> L.), leek (<em>Allium porrum</em> L.) and wild garlic (<em>Allium ursinum</em> L.). They contain many health beneficial substances, such as sulphur compounds, vitamins (vitamin C), mineral substances (Fe, Mg, Ca, P), polyphenols (especielly quercetin) and substances antioxidant activity and fiber. In this work we evaluated the content of bioactive substances, especially the content of total polyphenols and antioxidant activity in several species (red onion, yellow onion, white onion, garlic, leek, wild garlic) of the genus <em>Allium</em>. Samples of plant material were collected at full maturity stages from Zohor (Slovak Republic). Zohor is an area without negative influences and emission sources. Samples of fresh species of the genus <em>Allium</em> were homogenized and prepared as an extract: 25 g cut plants material (red onion, yellow onion, white onion, garlic, leek, wild garlic) extracted with 50 mL of 80% ethanol for sixteen hours. These extracts were used for analyzes. The content of total polyphenols was determined using the Folin-Ciocalteu reagent (FCR). The absorbance was measured at 765 nm of wavelength versus blank. Antioxidant activity was measured using DPPH˙ (2,2-difenyl-1-picrylhydrazyl) at 515.6 nm in the spectrophotometer. In the present experiment it was found that total polyphenols content in samples ranged from 83.59 mg.kg<sup>-1</sup> to 758.63 mg.kg<sup>-1</sup> and values of antioxidant activity were in the range from 7.19% to 53.55%.</p><p>&nbsp;</p> <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>EN-GB</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:SplitPgBreakAndParaMark /> <w:EnableOpenTypeKerning /> 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spelling doaj.art-f4b30df14e8748b1944faa07877d05eb2022-12-21T20:46:25ZengHACCP ConsultingPotravinarstvo1337-09602017-01-0111170270810.5219/833615Evaluation and comparison of bioactive substances in selected species of the genus AlliumJán Kovarovič0Judita Bystrická1Alexander Fehér2Marianna Lenková3Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Chemistry, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Chemistry, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture in Nitra, Faculty of European Studies and Regional Development, Department of Sustainable Development, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Department of Chemistry, Tr. A. Hlinku 2, 949 76 Nitra<!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:RelyOnVML /> <o:AllowPNG /> </o:OfficeDocumentSettings> </xml><![endif]--> <p class="PotText" style="line-height: 115%;"><em>Allium</em> is a genus of some 650 species belonging to the family <em>Liliaceae</em>. However only a few of these are important as food plants, notably garlic (<em>Allium sativum</em> L.), onion (<em>Allium cepa</em> L.), leek (<em>Allium porrum</em> L.) and wild garlic (<em>Allium ursinum</em> L.). They contain many health beneficial substances, such as sulphur compounds, vitamins (vitamin C), mineral substances (Fe, Mg, Ca, P), polyphenols (especielly quercetin) and substances antioxidant activity and fiber. In this work we evaluated the content of bioactive substances, especially the content of total polyphenols and antioxidant activity in several species (red onion, yellow onion, white onion, garlic, leek, wild garlic) of the genus <em>Allium</em>. Samples of plant material were collected at full maturity stages from Zohor (Slovak Republic). Zohor is an area without negative influences and emission sources. Samples of fresh species of the genus <em>Allium</em> were homogenized and prepared as an extract: 25 g cut plants material (red onion, yellow onion, white onion, garlic, leek, wild garlic) extracted with 50 mL of 80% ethanol for sixteen hours. These extracts were used for analyzes. The content of total polyphenols was determined using the Folin-Ciocalteu reagent (FCR). The absorbance was measured at 765 nm of wavelength versus blank. Antioxidant activity was measured using DPPH˙ (2,2-difenyl-1-picrylhydrazyl) at 515.6 nm in the spectrophotometer. In the present experiment it was found that total polyphenols content in samples ranged from 83.59 mg.kg<sup>-1</sup> to 758.63 mg.kg<sup>-1</sup> and values of antioxidant activity were in the range from 7.19% to 53.55%.</p><p>&nbsp;</p> <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>EN-GB</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:SplitPgBreakAndParaMark /> <w:EnableOpenTypeKerning /> 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spellingShingle Ján Kovarovič
Judita Bystrická
Alexander Fehér
Marianna Lenková
Evaluation and comparison of bioactive substances in selected species of the genus Allium
Potravinarstvo
polyphenols
antioxidant activity
Allium
species
variety
title Evaluation and comparison of bioactive substances in selected species of the genus Allium
title_full Evaluation and comparison of bioactive substances in selected species of the genus Allium
title_fullStr Evaluation and comparison of bioactive substances in selected species of the genus Allium
title_full_unstemmed Evaluation and comparison of bioactive substances in selected species of the genus Allium
title_short Evaluation and comparison of bioactive substances in selected species of the genus Allium
title_sort evaluation and comparison of bioactive substances in selected species of the genus allium
topic polyphenols
antioxidant activity
Allium
species
variety
url http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/833
work_keys_str_mv AT jankovarovic evaluationandcomparisonofbioactivesubstancesinselectedspeciesofthegenusallium
AT juditabystricka evaluationandcomparisonofbioactivesubstancesinselectedspeciesofthegenusallium
AT alexanderfeher evaluationandcomparisonofbioactivesubstancesinselectedspeciesofthegenusallium
AT mariannalenkova evaluationandcomparisonofbioactivesubstancesinselectedspeciesofthegenusallium