Formulation, characterization and biological evaluation of injectable nanocrystals from stem exudate gel of Caralluma retrospiciens (Ehrenb) – Part C
The purpose of this study was to develop injectable nanocrystals (NC) from stem exudate gel (EG) from Caralluma retrospiciens (Ehrenb) using the technique of nanoprecipitation. The NC had a zeta potential of −5.58 ± 4.27 mV. Size distribution analysis showed that it ranged in size from 100 to 300 nm...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535221005943 |
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| author | Muhammad H. Sultan Sivakumar S. Moni Saad S. Alqahtani Hafiz A. Makeen Osama A. Madkhali Mohammed Ali Bakkari Santhosh Joseph Menachery Yosif Almoshari Ahmad Salawi Meshal Alshamrani Awaji Y. Safhi Syam Mohan Mohamed Eltaib Elmobark |
| author_facet | Muhammad H. Sultan Sivakumar S. Moni Saad S. Alqahtani Hafiz A. Makeen Osama A. Madkhali Mohammed Ali Bakkari Santhosh Joseph Menachery Yosif Almoshari Ahmad Salawi Meshal Alshamrani Awaji Y. Safhi Syam Mohan Mohamed Eltaib Elmobark |
| author_sort | Muhammad H. Sultan |
| collection | DOAJ |
| description | The purpose of this study was to develop injectable nanocrystals (NC) from stem exudate gel (EG) from Caralluma retrospiciens (Ehrenb) using the technique of nanoprecipitation. The NC had a zeta potential of −5.58 ± 4.27 mV. Size distribution analysis showed that it ranged in size from 100 to 300 nm. The polydispersity index (PDI) was 0.467, while its percentage PDI was 68.4. Scanning electron microscopic analysis and transmission electron microscopy studies revealed the morphological features of NC as discrete crystals with rough surfaces. The mobility of NC was 5.5 µm.cm/Vs, while its conductivity was 0.16 mS/cm. Antibacterial studies showed broad activity against both Gram-positive and Gram-negative bacteria. The minimum inhibitory concentrations (MICs) of NC against Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa) and Klebsiella pneumoniae (K. pneumoniae) were 6, 8, 8, 4, 8, and 6 % (w/v), respectively. The antibacterial effect was highest against K. pneumoniae (25.6 ± 1.5 mm), followed by E. coli (25.5 ± 1.8 mm), P. aeruginosa (24.1 ± 1.2 mm), S. pyogenes (22.2 ± 1.2 mm), S. aureus (21.83 ± 1.2 mm) and B. subtilis (20.33 ± 1.8 mm). In this study, the cytotoxicity properties of NC were determined against MCF-7 breast cancer cells ATCC. The NC failed to inhibit the proliferation of MCF-7 cells against the even at 300 µg/mL concentration. These results indicated that the NC is a promising antibacterial injectable dosage form. |
| first_indexed | 2024-12-17T17:39:43Z |
| format | Article |
| id | doaj.art-f4b4016bba6f4eb9af3ebb05609054a7 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-17T17:39:43Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-f4b4016bba6f4eb9af3ebb05609054a72022-12-21T21:39:13ZengElsevierArabian Journal of Chemistry1878-53522022-02-01152103579Formulation, characterization and biological evaluation of injectable nanocrystals from stem exudate gel of Caralluma retrospiciens (Ehrenb) – Part CMuhammad H. Sultan0Sivakumar S. Moni1Saad S. Alqahtani2Hafiz A. Makeen3Osama A. Madkhali4Mohammed Ali Bakkari5Santhosh Joseph Menachery6Yosif Almoshari7Ahmad Salawi8Meshal Alshamrani9Awaji Y. Safhi10Syam Mohan11Mohamed Eltaib Elmobark12Department of Pharmaceutics, Faculty of Pharmacy, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, Jazan University, Jazan, Saudi Arabia; Corresponding authors.Pharmacy Practice Research Unit, Clinical Pharmacy Department, Faculty of Pharmacy, Jazan University, Jazan, Saudi ArabiaPharmacy Practice Research Unit, Clinical Pharmacy Department, Faculty of Pharmacy, Jazan University, Jazan, Saudi Arabia; Corresponding authors.Department of Pharmaceutics, Faculty of Pharmacy, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, Jazan University, Jazan, Saudi ArabiaPharmacy Practice Research Unit, Clinical Pharmacy Department, Faculty of Pharmacy, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, Jazan University, Jazan, Saudi ArabiaSubstance Abuse and Toxicology Research Centre, Jazan University, Jazan, Saudi ArabiaDepartment of Pharmaceutics, Faculty of Pharmacy, Jazan University, Jazan, Saudi ArabiaThe purpose of this study was to develop injectable nanocrystals (NC) from stem exudate gel (EG) from Caralluma retrospiciens (Ehrenb) using the technique of nanoprecipitation. The NC had a zeta potential of −5.58 ± 4.27 mV. Size distribution analysis showed that it ranged in size from 100 to 300 nm. The polydispersity index (PDI) was 0.467, while its percentage PDI was 68.4. Scanning electron microscopic analysis and transmission electron microscopy studies revealed the morphological features of NC as discrete crystals with rough surfaces. The mobility of NC was 5.5 µm.cm/Vs, while its conductivity was 0.16 mS/cm. Antibacterial studies showed broad activity against both Gram-positive and Gram-negative bacteria. The minimum inhibitory concentrations (MICs) of NC against Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa) and Klebsiella pneumoniae (K. pneumoniae) were 6, 8, 8, 4, 8, and 6 % (w/v), respectively. The antibacterial effect was highest against K. pneumoniae (25.6 ± 1.5 mm), followed by E. coli (25.5 ± 1.8 mm), P. aeruginosa (24.1 ± 1.2 mm), S. pyogenes (22.2 ± 1.2 mm), S. aureus (21.83 ± 1.2 mm) and B. subtilis (20.33 ± 1.8 mm). In this study, the cytotoxicity properties of NC were determined against MCF-7 breast cancer cells ATCC. The NC failed to inhibit the proliferation of MCF-7 cells against the even at 300 µg/mL concentration. These results indicated that the NC is a promising antibacterial injectable dosage form.http://www.sciencedirect.com/science/article/pii/S1878535221005943NanocrystalsExudate gelDesert plantCaralluma retrospiciensAntibacterial properties |
| spellingShingle | Muhammad H. Sultan Sivakumar S. Moni Saad S. Alqahtani Hafiz A. Makeen Osama A. Madkhali Mohammed Ali Bakkari Santhosh Joseph Menachery Yosif Almoshari Ahmad Salawi Meshal Alshamrani Awaji Y. Safhi Syam Mohan Mohamed Eltaib Elmobark Formulation, characterization and biological evaluation of injectable nanocrystals from stem exudate gel of Caralluma retrospiciens (Ehrenb) – Part C Arabian Journal of Chemistry Nanocrystals Exudate gel Desert plant Caralluma retrospiciens Antibacterial properties |
| title | Formulation, characterization and biological evaluation of injectable nanocrystals from stem exudate gel of Caralluma retrospiciens (Ehrenb) – Part C |
| title_full | Formulation, characterization and biological evaluation of injectable nanocrystals from stem exudate gel of Caralluma retrospiciens (Ehrenb) – Part C |
| title_fullStr | Formulation, characterization and biological evaluation of injectable nanocrystals from stem exudate gel of Caralluma retrospiciens (Ehrenb) – Part C |
| title_full_unstemmed | Formulation, characterization and biological evaluation of injectable nanocrystals from stem exudate gel of Caralluma retrospiciens (Ehrenb) – Part C |
| title_short | Formulation, characterization and biological evaluation of injectable nanocrystals from stem exudate gel of Caralluma retrospiciens (Ehrenb) – Part C |
| title_sort | formulation characterization and biological evaluation of injectable nanocrystals from stem exudate gel of caralluma retrospiciens ehrenb part c |
| topic | Nanocrystals Exudate gel Desert plant Caralluma retrospiciens Antibacterial properties |
| url | http://www.sciencedirect.com/science/article/pii/S1878535221005943 |
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