A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine
Ossification of the ligamentum flavum (OLF) is a common spinal disorder that causes myelopathy and radiculopathy. Non-coding RNAs (ncRNAs) are involved in numerous pathological processes; however, very few ncRNAs have been identified to be correlated with OLF. Here we compared the expression of lncR...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-09-01
|
| Series: | Molecular Therapy: Nucleic Acids |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S216225311830204X |
| _version_ | 1818197628515516416 |
|---|---|
| author | Yawei Han Yuheng Hong Liandong Li Tengshuai Li Zhen Zhang Jingzhao Wang Han Xia Yutao Tang Zhemin Shi Xiaohui Han Ting Chen Qi Liu Mengxia Zhang Kun Zhang Wei Hong Yuan Xue |
| author_facet | Yawei Han Yuheng Hong Liandong Li Tengshuai Li Zhen Zhang Jingzhao Wang Han Xia Yutao Tang Zhemin Shi Xiaohui Han Ting Chen Qi Liu Mengxia Zhang Kun Zhang Wei Hong Yuan Xue |
| author_sort | Yawei Han |
| collection | DOAJ |
| description | Ossification of the ligamentum flavum (OLF) is a common spinal disorder that causes myelopathy and radiculopathy. Non-coding RNAs (ncRNAs) are involved in numerous pathological processes; however, very few ncRNAs have been identified to be correlated with OLF. Here we compared the expression of lncRNA, mRNA, circRNA, and microRNA in OLF tissues from OLF patients and healthy volunteers through mRNA, lncRNA, and circRNA microarrays and microRNA sequencing. A total of 2,054 mRNAs, 2,567 lncRNAs, 627 circRNAs, and 28 microRNAs (miRNAs) were altered during the process of OLF. qPCR confirmed the differential expression of selected mRNAs and ncRNAs. An lncRNA-mRNA co-expression network, miRNA-mRNA target prediction network, and competing endogenous RNA (ceRNA) network of circRNA-miRNA-mRNA were constructed based on a correlation analysis of the differentially expressed RNA transcripts. Subsequently, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses for the differentially expressed mRNAs and the predicted miRNAs target genes were performed. In addition, a deregulated miRNA-19b-3p-based miRNA-circRNA-lncRNA-mRNA network was confirmed, by gain-of-function and loss-of-function experiments, to function in the process of ossification. Taken together, this study provides a systematic perspective on the potential function of ncRNAs in the pathogenesis of OLF. Keywords: non-coding RNA, ossification, ligamentum flavum, lncRNA, microRNA, circRNA, transcriptome |
| first_indexed | 2024-12-12T01:53:00Z |
| format | Article |
| id | doaj.art-f4b7694006c84171a9e81a7e9a92252b |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-12-12T01:53:00Z |
| publishDate | 2018-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-f4b7694006c84171a9e81a7e9a92252b2022-12-22T00:42:26ZengElsevierMolecular Therapy: Nucleic Acids2162-25312018-09-0112872883A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the SpineYawei Han0Yuheng Hong1Liandong Li2Tengshuai Li3Zhen Zhang4Jingzhao Wang5Han Xia6Yutao Tang7Zhemin Shi8Xiaohui Han9Ting Chen10Qi Liu11Mengxia Zhang12Kun Zhang13Wei Hong14Yuan Xue15Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaSchool of Medical Imaging, Tianjin Medical University, Tianjin, ChinaDepartment of Orthopaedics, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Orthopaedics, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Orthopaedics, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Orthopaedics, Tianjin Medical University General Hospital, Tianjin, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China; Corresponding author: Wei Hong, Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, No. 22 Qixiangtai Road, Heping District, Tianjin, China.Department of Orthopaedics, Tianjin Medical University General Hospital, Tianjin, China; Corresponding author: Yuan Xue, Department of Orthopaedics, Tianjin Medical University General Hospital, Tianjin, China.Ossification of the ligamentum flavum (OLF) is a common spinal disorder that causes myelopathy and radiculopathy. Non-coding RNAs (ncRNAs) are involved in numerous pathological processes; however, very few ncRNAs have been identified to be correlated with OLF. Here we compared the expression of lncRNA, mRNA, circRNA, and microRNA in OLF tissues from OLF patients and healthy volunteers through mRNA, lncRNA, and circRNA microarrays and microRNA sequencing. A total of 2,054 mRNAs, 2,567 lncRNAs, 627 circRNAs, and 28 microRNAs (miRNAs) were altered during the process of OLF. qPCR confirmed the differential expression of selected mRNAs and ncRNAs. An lncRNA-mRNA co-expression network, miRNA-mRNA target prediction network, and competing endogenous RNA (ceRNA) network of circRNA-miRNA-mRNA were constructed based on a correlation analysis of the differentially expressed RNA transcripts. Subsequently, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses for the differentially expressed mRNAs and the predicted miRNAs target genes were performed. In addition, a deregulated miRNA-19b-3p-based miRNA-circRNA-lncRNA-mRNA network was confirmed, by gain-of-function and loss-of-function experiments, to function in the process of ossification. Taken together, this study provides a systematic perspective on the potential function of ncRNAs in the pathogenesis of OLF. Keywords: non-coding RNA, ossification, ligamentum flavum, lncRNA, microRNA, circRNA, transcriptomehttp://www.sciencedirect.com/science/article/pii/S216225311830204X |
| spellingShingle | Yawei Han Yuheng Hong Liandong Li Tengshuai Li Zhen Zhang Jingzhao Wang Han Xia Yutao Tang Zhemin Shi Xiaohui Han Ting Chen Qi Liu Mengxia Zhang Kun Zhang Wei Hong Yuan Xue A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine Molecular Therapy: Nucleic Acids |
| title | A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine |
| title_full | A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine |
| title_fullStr | A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine |
| title_full_unstemmed | A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine |
| title_short | A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine |
| title_sort | transcriptome level study identifies changing expression profiles for ossification of the ligamentum flavum of the spine |
| url | http://www.sciencedirect.com/science/article/pii/S216225311830204X |
| work_keys_str_mv | AT yaweihan atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT yuhenghong atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT liandongli atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT tengshuaili atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT zhenzhang atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT jingzhaowang atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT hanxia atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT yutaotang atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT zheminshi atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT xiaohuihan atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT tingchen atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT qiliu atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT mengxiazhang atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT kunzhang atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT weihong atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT yuanxue atranscriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT yaweihan transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT yuhenghong transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT liandongli transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT tengshuaili transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT zhenzhang transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT jingzhaowang transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT hanxia transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT yutaotang transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT zheminshi transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT xiaohuihan transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT tingchen transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT qiliu transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT mengxiazhang transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT kunzhang transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT weihong transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine AT yuanxue transcriptomelevelstudyidentifieschangingexpressionprofilesforossificationoftheligamentumflavumofthespine |