N Addition Overwhelmed the Effects of P Addition on the Soil C, N, and P Cycling Genes in Alpine Meadow of the Qinghai-Tibetan Plateau
Although human activities have greatly increased nitrogen (N) and phosphorus (P) inputs to the alpine grassland ecosystems, how soil microbial functional genes involved in nutrient cycling respond to N and P input remains unknown. Based on a fertilization experiment established in an alpine meadow o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.860590/full |
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| author | Jiannan Xiao Shikui Dong Hao Shen Shuai Li Kelly Wessell Shiliang Liu Wei Li Yangliu Zhi Zhiyuan Mu Hongbo Li |
| author_facet | Jiannan Xiao Shikui Dong Hao Shen Shuai Li Kelly Wessell Shiliang Liu Wei Li Yangliu Zhi Zhiyuan Mu Hongbo Li |
| author_sort | Jiannan Xiao |
| collection | DOAJ |
| description | Although human activities have greatly increased nitrogen (N) and phosphorus (P) inputs to the alpine grassland ecosystems, how soil microbial functional genes involved in nutrient cycling respond to N and P input remains unknown. Based on a fertilization experiment established in an alpine meadow of the Qinghai-Tibetan Plateau, we investigated the response of the abundance of soil carbon (C), N, and P cycling genes to N and P addition and evaluated soil and plant factors related to the observed effects. Our results indicated that the abundance of C, N, and P cycling genes were hardly affected by N addition, while P addition significantly increased most of them, suggesting that the availability of P plays a more important role for soil microorganisms than N in this alpine meadow ecosystem. Meanwhile, when N and P were added together, the abundance of C, N, and P cycling genes did not change significantly, indicating that the promoting effects of P addition on microbial functional genes abundances were overwhelmed by N addition. The Mantel analysis and the variation partitioning analysis revealed the major role of shoot P concentration in regulating the abundance of C, N, and P cycling genes. These results suggest that soil P availability and plant traits are key in governing C, N, and P cycling genes at the functional gene level in the alpine grassland ecosystem. |
| first_indexed | 2024-04-14T01:15:28Z |
| format | Article |
| id | doaj.art-f4b99659426147698b66126b8e8c8ace |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-14T01:15:28Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f4b99659426147698b66126b8e8c8ace2022-12-22T02:20:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-04-011310.3389/fpls.2022.860590860590N Addition Overwhelmed the Effects of P Addition on the Soil C, N, and P Cycling Genes in Alpine Meadow of the Qinghai-Tibetan PlateauJiannan Xiao0Shikui Dong1Hao Shen2Shuai Li3Kelly Wessell4Shiliang Liu5Wei Li6Yangliu Zhi7Zhiyuan Mu8Hongbo Li9School of Environment, Beijing Normal University, Beijing, ChinaSchool of Grassland Sciences, Beijing Forestry University, Beijing, ChinaSchool of Grassland Sciences, Beijing Forestry University, Beijing, ChinaCollege of Resource and Environment, Shanxi Agricultural University, Taigu, ChinaTompkin Cortland Community College, Ithaca, NY, United StatesSchool of Environment, Beijing Normal University, Beijing, ChinaSchool of Environment, Beijing Normal University, Beijing, ChinaSchool of Environment, Beijing Normal University, Beijing, ChinaSchool of Environment, Beijing Normal University, Beijing, ChinaInstitute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, ChinaAlthough human activities have greatly increased nitrogen (N) and phosphorus (P) inputs to the alpine grassland ecosystems, how soil microbial functional genes involved in nutrient cycling respond to N and P input remains unknown. Based on a fertilization experiment established in an alpine meadow of the Qinghai-Tibetan Plateau, we investigated the response of the abundance of soil carbon (C), N, and P cycling genes to N and P addition and evaluated soil and plant factors related to the observed effects. Our results indicated that the abundance of C, N, and P cycling genes were hardly affected by N addition, while P addition significantly increased most of them, suggesting that the availability of P plays a more important role for soil microorganisms than N in this alpine meadow ecosystem. Meanwhile, when N and P were added together, the abundance of C, N, and P cycling genes did not change significantly, indicating that the promoting effects of P addition on microbial functional genes abundances were overwhelmed by N addition. The Mantel analysis and the variation partitioning analysis revealed the major role of shoot P concentration in regulating the abundance of C, N, and P cycling genes. These results suggest that soil P availability and plant traits are key in governing C, N, and P cycling genes at the functional gene level in the alpine grassland ecosystem.https://www.frontiersin.org/articles/10.3389/fpls.2022.860590/fullmicrobial functional genenitrogen additionphosphorus additionbiogeochemical cyclingalpine grassland |
| spellingShingle | Jiannan Xiao Shikui Dong Hao Shen Shuai Li Kelly Wessell Shiliang Liu Wei Li Yangliu Zhi Zhiyuan Mu Hongbo Li N Addition Overwhelmed the Effects of P Addition on the Soil C, N, and P Cycling Genes in Alpine Meadow of the Qinghai-Tibetan Plateau Frontiers in Plant Science microbial functional gene nitrogen addition phosphorus addition biogeochemical cycling alpine grassland |
| title | N Addition Overwhelmed the Effects of P Addition on the Soil C, N, and P Cycling Genes in Alpine Meadow of the Qinghai-Tibetan Plateau |
| title_full | N Addition Overwhelmed the Effects of P Addition on the Soil C, N, and P Cycling Genes in Alpine Meadow of the Qinghai-Tibetan Plateau |
| title_fullStr | N Addition Overwhelmed the Effects of P Addition on the Soil C, N, and P Cycling Genes in Alpine Meadow of the Qinghai-Tibetan Plateau |
| title_full_unstemmed | N Addition Overwhelmed the Effects of P Addition on the Soil C, N, and P Cycling Genes in Alpine Meadow of the Qinghai-Tibetan Plateau |
| title_short | N Addition Overwhelmed the Effects of P Addition on the Soil C, N, and P Cycling Genes in Alpine Meadow of the Qinghai-Tibetan Plateau |
| title_sort | n addition overwhelmed the effects of p addition on the soil c n and p cycling genes in alpine meadow of the qinghai tibetan plateau |
| topic | microbial functional gene nitrogen addition phosphorus addition biogeochemical cycling alpine grassland |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.860590/full |
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