Optimization of TBAB-Assisted Lipase-catalyzed Synthesis of L-Ascorbyl Myristate
The synthesis of l-ascorbyl myristate through esterification of l-ascorbic acid and myristic acid catalyzed by lipase was studied. The effect of different parameters including lipase, molecular sieve 4 Å, organic solvent, and tetrabutyl ammonium bromide (TBAB) was evaluated. The results showed exce...
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| Format: | Article |
| Language: | English |
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Croatian Society of Chemical Engineers
2018-07-01
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| Series: | Chemical and Biochemical Engineering Quarterly |
| Subjects: | |
| Online Access: | http://silverstripe.fkit.hr/cabeq/assets/Uploads/09-2-18.pdf |
| _version_ | 1818348948619788288 |
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| author | H. Li L. Zhang R. Shen Y. Liu |
| author_facet | H. Li L. Zhang R. Shen Y. Liu |
| author_sort | H. Li |
| collection | DOAJ |
| description | The synthesis of l-ascorbyl myristate through esterification of l-ascorbic acid and myristic acid catalyzed by lipase was studied. The effect of different parameters including lipase, molecular sieve 4 Å, organic solvent, and tetrabutyl ammonium bromide (TBAB)
was evaluated. The results showed excellent yield in the presence of Novozyme435 as catalyst, 10 % (w/w of l-ascorbic acid) of 4 Å molecular sieve and 10 % (w/w of l-ascorbic acid) of TBAB as additives in 1,4-dioxane. Furthermore, response surface methodology (RSM) and 5-level-4-factor central composite design (CCD) were employed to evaluate the effects of parameters on the yield of l-ascorbyl myristate. According to the analysis result of ridge max, the optimal synthesis conditions were predicted, as follows: reaction time 27.1 h, temperature 54.1 °C, enzyme amount 25.9 % (% w/w of l-ascorbic acid), substrate molar ratio 5, and the optimal actual yield was 85.0 %. |
| first_indexed | 2024-12-13T17:58:10Z |
| format | Article |
| id | doaj.art-f4b9dc16586a41b186d3dc59afe7928a |
| institution | Directory Open Access Journal |
| issn | 0352-9568 1846-5153 |
| language | English |
| last_indexed | 2024-12-13T17:58:10Z |
| publishDate | 2018-07-01 |
| publisher | Croatian Society of Chemical Engineers |
| record_format | Article |
| series | Chemical and Biochemical Engineering Quarterly |
| spelling | doaj.art-f4b9dc16586a41b186d3dc59afe7928a2022-12-21T23:36:18ZengCroatian Society of Chemical EngineersChemical and Biochemical Engineering Quarterly0352-95681846-51532018-07-0132224725510.15255/CABEQ.2016.978Optimization of TBAB-Assisted Lipase-catalyzed Synthesis of L-Ascorbyl MyristateH. Li0 L. Zhang1R. Shen2Y. Liu3a)School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, China; b)Henan Collaborative Innovation Center for Food Production and Safety, Zhengzhou, Henan Province, Chinaa)School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, China; b)Henan Collaborative Innovation Center for Food Production and Safety, Zhengzhou, Henan Province, Chinaa)School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, China; b)Henan Collaborative Innovation Center for Food Production and Safety, Zhengzhou, Henan Province, Chinaa)School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, China; b)Henan Collaborative Innovation Center for Food Production and Safety, Zhengzhou, Henan Province, ChinaThe synthesis of l-ascorbyl myristate through esterification of l-ascorbic acid and myristic acid catalyzed by lipase was studied. The effect of different parameters including lipase, molecular sieve 4 Å, organic solvent, and tetrabutyl ammonium bromide (TBAB) was evaluated. The results showed excellent yield in the presence of Novozyme435 as catalyst, 10 % (w/w of l-ascorbic acid) of 4 Å molecular sieve and 10 % (w/w of l-ascorbic acid) of TBAB as additives in 1,4-dioxane. Furthermore, response surface methodology (RSM) and 5-level-4-factor central composite design (CCD) were employed to evaluate the effects of parameters on the yield of l-ascorbyl myristate. According to the analysis result of ridge max, the optimal synthesis conditions were predicted, as follows: reaction time 27.1 h, temperature 54.1 °C, enzyme amount 25.9 % (% w/w of l-ascorbic acid), substrate molar ratio 5, and the optimal actual yield was 85.0 %.http://silverstripe.fkit.hr/cabeq/assets/Uploads/09-2-18.pdfl-ascorbyl myristateNovozyme435TBABResponse Surface Methodology |
| spellingShingle | H. Li L. Zhang R. Shen Y. Liu Optimization of TBAB-Assisted Lipase-catalyzed Synthesis of L-Ascorbyl Myristate Chemical and Biochemical Engineering Quarterly l-ascorbyl myristate Novozyme435 TBAB Response Surface Methodology |
| title | Optimization of TBAB-Assisted Lipase-catalyzed Synthesis of L-Ascorbyl Myristate |
| title_full | Optimization of TBAB-Assisted Lipase-catalyzed Synthesis of L-Ascorbyl Myristate |
| title_fullStr | Optimization of TBAB-Assisted Lipase-catalyzed Synthesis of L-Ascorbyl Myristate |
| title_full_unstemmed | Optimization of TBAB-Assisted Lipase-catalyzed Synthesis of L-Ascorbyl Myristate |
| title_short | Optimization of TBAB-Assisted Lipase-catalyzed Synthesis of L-Ascorbyl Myristate |
| title_sort | optimization of tbab assisted lipase catalyzed synthesis of l ascorbyl myristate |
| topic | l-ascorbyl myristate Novozyme435 TBAB Response Surface Methodology |
| url | http://silverstripe.fkit.hr/cabeq/assets/Uploads/09-2-18.pdf |
| work_keys_str_mv | AT hli optimizationoftbabassistedlipasecatalyzedsynthesisoflascorbylmyristate AT lzhang optimizationoftbabassistedlipasecatalyzedsynthesisoflascorbylmyristate AT rshen optimizationoftbabassistedlipasecatalyzedsynthesisoflascorbylmyristate AT yliu optimizationoftbabassistedlipasecatalyzedsynthesisoflascorbylmyristate |