Label-Free, High-Throughput Assay of Human Dendritic Cells from Whole-Blood Samples with Microfluidic Inertial Separation Suitable for Resource-Limited Manufacturing
Microfluidics technology has not impacted the delivery and accessibility of point-of-care health services, like diagnosing infectious disease, monitoring health or delivering interventions. Most microfluidics prototypes in academic research are not easy to scale-up with industrial-scale fabrication...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/11/5/514 |
| _version_ | 1797567603366952960 |
|---|---|
| author | Mohamed Yousuff Caffiyar Kue Peng Lim Ismail Hussain Kamal Basha Nor Hisham Hamid Sok Ching Cheong Eric Tatt Wei Ho |
| author_facet | Mohamed Yousuff Caffiyar Kue Peng Lim Ismail Hussain Kamal Basha Nor Hisham Hamid Sok Ching Cheong Eric Tatt Wei Ho |
| author_sort | Mohamed Yousuff Caffiyar |
| collection | DOAJ |
| description | Microfluidics technology has not impacted the delivery and accessibility of point-of-care health services, like diagnosing infectious disease, monitoring health or delivering interventions. Most microfluidics prototypes in academic research are not easy to scale-up with industrial-scale fabrication techniques and cannot be operated without complex manipulations of supporting equipment and additives, such as labels or reagents. We propose a label- and reagent-free inertial spiral microfluidic device to separate red blood, white blood and dendritic cells from blood fluid, for applications in health monitoring and immunotherapy. We demonstrate that using larger channel widths, in the range of 200 to 600 µm, allows separation of cells into multiple focused streams, according to different size ranges, and we utilize a novel technique to collect the closely separated focused cell streams, without constricting the channel. Our contribution is a method to adapt spiral inertial microfluidic designs to separate more than two cell types in the same device, which is robust against clogging, simple to operate and suitable for fabrication and deployment in resource-limited populations. When tested on actual human blood cells, 77% of dendritic cells were separated and 80% of cells remained viable after our assay. |
| first_indexed | 2024-03-10T19:44:17Z |
| format | Article |
| id | doaj.art-f4bb754702574dce865863733ce1492a |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T19:44:17Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-f4bb754702574dce865863733ce1492a2023-11-20T00:59:34ZengMDPI AGMicromachines2072-666X2020-05-0111551410.3390/mi11050514Label-Free, High-Throughput Assay of Human Dendritic Cells from Whole-Blood Samples with Microfluidic Inertial Separation Suitable for Resource-Limited ManufacturingMohamed Yousuff Caffiyar0Kue Peng Lim1Ismail Hussain Kamal Basha2Nor Hisham Hamid3Sok Ching Cheong4Eric Tatt Wei Ho5Department of Electrical & Electronics Engineering, Universiti Teknologi PETRONAS, Perak 32610, MalaysiaHead and Neck Cancer Research Group, Cancer Research Malaysia, Selangor 47500, MalaysiaDepartment of Electrical & Electronics Engineering, Universiti Teknologi PETRONAS, Perak 32610, MalaysiaDepartment of Electrical & Electronics Engineering, Universiti Teknologi PETRONAS, Perak 32610, MalaysiaHead and Neck Cancer Research Group, Cancer Research Malaysia, Selangor 47500, MalaysiaDepartment of Electrical & Electronics Engineering, Universiti Teknologi PETRONAS, Perak 32610, MalaysiaMicrofluidics technology has not impacted the delivery and accessibility of point-of-care health services, like diagnosing infectious disease, monitoring health or delivering interventions. Most microfluidics prototypes in academic research are not easy to scale-up with industrial-scale fabrication techniques and cannot be operated without complex manipulations of supporting equipment and additives, such as labels or reagents. We propose a label- and reagent-free inertial spiral microfluidic device to separate red blood, white blood and dendritic cells from blood fluid, for applications in health monitoring and immunotherapy. We demonstrate that using larger channel widths, in the range of 200 to 600 µm, allows separation of cells into multiple focused streams, according to different size ranges, and we utilize a novel technique to collect the closely separated focused cell streams, without constricting the channel. Our contribution is a method to adapt spiral inertial microfluidic designs to separate more than two cell types in the same device, which is robust against clogging, simple to operate and suitable for fabrication and deployment in resource-limited populations. When tested on actual human blood cells, 77% of dendritic cells were separated and 80% of cells remained viable after our assay.https://www.mdpi.com/2072-666X/11/5/514inertial spiral microfluidicsdendritic cell separationresource-limited microfluidics |
| spellingShingle | Mohamed Yousuff Caffiyar Kue Peng Lim Ismail Hussain Kamal Basha Nor Hisham Hamid Sok Ching Cheong Eric Tatt Wei Ho Label-Free, High-Throughput Assay of Human Dendritic Cells from Whole-Blood Samples with Microfluidic Inertial Separation Suitable for Resource-Limited Manufacturing Micromachines inertial spiral microfluidics dendritic cell separation resource-limited microfluidics |
| title | Label-Free, High-Throughput Assay of Human Dendritic Cells from Whole-Blood Samples with Microfluidic Inertial Separation Suitable for Resource-Limited Manufacturing |
| title_full | Label-Free, High-Throughput Assay of Human Dendritic Cells from Whole-Blood Samples with Microfluidic Inertial Separation Suitable for Resource-Limited Manufacturing |
| title_fullStr | Label-Free, High-Throughput Assay of Human Dendritic Cells from Whole-Blood Samples with Microfluidic Inertial Separation Suitable for Resource-Limited Manufacturing |
| title_full_unstemmed | Label-Free, High-Throughput Assay of Human Dendritic Cells from Whole-Blood Samples with Microfluidic Inertial Separation Suitable for Resource-Limited Manufacturing |
| title_short | Label-Free, High-Throughput Assay of Human Dendritic Cells from Whole-Blood Samples with Microfluidic Inertial Separation Suitable for Resource-Limited Manufacturing |
| title_sort | label free high throughput assay of human dendritic cells from whole blood samples with microfluidic inertial separation suitable for resource limited manufacturing |
| topic | inertial spiral microfluidics dendritic cell separation resource-limited microfluidics |
| url | https://www.mdpi.com/2072-666X/11/5/514 |
| work_keys_str_mv | AT mohamedyousuffcaffiyar labelfreehighthroughputassayofhumandendriticcellsfromwholebloodsampleswithmicrofluidicinertialseparationsuitableforresourcelimitedmanufacturing AT kuepenglim labelfreehighthroughputassayofhumandendriticcellsfromwholebloodsampleswithmicrofluidicinertialseparationsuitableforresourcelimitedmanufacturing AT ismailhussainkamalbasha labelfreehighthroughputassayofhumandendriticcellsfromwholebloodsampleswithmicrofluidicinertialseparationsuitableforresourcelimitedmanufacturing AT norhishamhamid labelfreehighthroughputassayofhumandendriticcellsfromwholebloodsampleswithmicrofluidicinertialseparationsuitableforresourcelimitedmanufacturing AT sokchingcheong labelfreehighthroughputassayofhumandendriticcellsfromwholebloodsampleswithmicrofluidicinertialseparationsuitableforresourcelimitedmanufacturing AT erictattweiho labelfreehighthroughputassayofhumandendriticcellsfromwholebloodsampleswithmicrofluidicinertialseparationsuitableforresourcelimitedmanufacturing |