Effects of Sodium Azide, Barium Ion, d-Amphetamine and Procaine on Inward Rectifying Potassium Channel 6.2 Expressed in Xenopus Oocytes
Background/Purpose: Inward rectifying potassium channel 6.2 (Kir6.2 Δ C26 channel) is closely related to ATP-sensitive potassium channels. Whether sodium azide, barium ion, d-amphetamine or procaine acts directly on the Kir6.2 Δ C26 channel remains unclear. We studied the effects of these compounds...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2008-08-01
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| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664608601771 |
| _version_ | 1811310639596961792 |
|---|---|
| author | Fan-Lu Kung Jung-Lung Tsai Chien-Hsing Lee Kuo-Long Lou Chih-Yung Tang Horng-Huei Liou Kuan-Ling Lu Yi-Hung Chen Wun-Jheng Wang Ming-Cheng Tsai |
| author_facet | Fan-Lu Kung Jung-Lung Tsai Chien-Hsing Lee Kuo-Long Lou Chih-Yung Tang Horng-Huei Liou Kuan-Ling Lu Yi-Hung Chen Wun-Jheng Wang Ming-Cheng Tsai |
| author_sort | Fan-Lu Kung |
| collection | DOAJ |
| description | Background/Purpose: Inward rectifying potassium channel 6.2 (Kir6.2 Δ C26 channel) is closely related to ATP-sensitive potassium channels. Whether sodium azide, barium ion, d-amphetamine or procaine acts directly on the Kir6.2 Δ C26 channel remains unclear. We studied the effects of these compounds on Kir6.2 Δ C26 channel expressed in Xenopus oocytes.
Methods: The coding sequence of a truncated form of mouse Kir6.2 (GenBank accession number NP_034732.1), Kir6.21-364 (i.e. Kir6.2 Δ C26), was subcloned into the pET20b(+) vector. Plasmid containing the correct T7 promoter-Kir6.21-364 cDNA fragment [Kir6.2/pET20b(+)] was then subject to Not I digestion to generate the templates for in vitro run-off transcriptions. The channel was expressed in Xenopus laevis oocytes. Two-electrode voltage clamping was used to measure the effects of sodium azide, barium ion, d-amphetamine and procaine on Kir6.2 Δ C26 channel current.
Results: Sodium azide activated and barium ion and d-amphetamine inhibited the Kir6.2 Δ C26 channel. Procaine did not have any significant effect on the Kir6.2 Δ C26 channel.
Conclusion: Kir6.2 Δ C26 channel expressed in Xenopus oocytes can be used as a pharmacological tool for the study of inward rectifying potassium channels. |
| first_indexed | 2024-04-13T10:02:26Z |
| format | Article |
| id | doaj.art-f4bc47890e5a48a9bb418c136a1ff423 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-04-13T10:02:26Z |
| publishDate | 2008-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-f4bc47890e5a48a9bb418c136a1ff4232022-12-22T02:51:12ZengElsevierJournal of the Formosan Medical Association0929-66462008-08-01107860060810.1016/S0929-6646(08)60177-1Effects of Sodium Azide, Barium Ion, d-Amphetamine and Procaine on Inward Rectifying Potassium Channel 6.2 Expressed in Xenopus OocytesFan-Lu Kung0Jung-Lung Tsai1Chien-Hsing Lee2Kuo-Long Lou3Chih-Yung Tang4Horng-Huei Liou5Kuan-Ling Lu6Yi-Hung Chen7Wun-Jheng Wang8Ming-Cheng Tsai9School of Pharmacy, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Pharmacology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Pharmacology, College of Medicine, National Taiwan University, Taipei, TaiwanInstitutes of Oral Biology, College of Medicine, National Taiwan University, Taipei, TaiwanInstitutes of Physiology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Pharmacology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Pharmacology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Pharmacology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Pharmacology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Pharmacology, College of Medicine, National Taiwan University, Taipei, TaiwanBackground/Purpose: Inward rectifying potassium channel 6.2 (Kir6.2 Δ C26 channel) is closely related to ATP-sensitive potassium channels. Whether sodium azide, barium ion, d-amphetamine or procaine acts directly on the Kir6.2 Δ C26 channel remains unclear. We studied the effects of these compounds on Kir6.2 Δ C26 channel expressed in Xenopus oocytes. Methods: The coding sequence of a truncated form of mouse Kir6.2 (GenBank accession number NP_034732.1), Kir6.21-364 (i.e. Kir6.2 Δ C26), was subcloned into the pET20b(+) vector. Plasmid containing the correct T7 promoter-Kir6.21-364 cDNA fragment [Kir6.2/pET20b(+)] was then subject to Not I digestion to generate the templates for in vitro run-off transcriptions. The channel was expressed in Xenopus laevis oocytes. Two-electrode voltage clamping was used to measure the effects of sodium azide, barium ion, d-amphetamine and procaine on Kir6.2 Δ C26 channel current. Results: Sodium azide activated and barium ion and d-amphetamine inhibited the Kir6.2 Δ C26 channel. Procaine did not have any significant effect on the Kir6.2 Δ C26 channel. Conclusion: Kir6.2 Δ C26 channel expressed in Xenopus oocytes can be used as a pharmacological tool for the study of inward rectifying potassium channels.http://www.sciencedirect.com/science/article/pii/S0929664608601771amphetaminebarium ioninward rectifying potassium channelKir6.2 KATPmRNAprocainesodium azideXenopus oocyte |
| spellingShingle | Fan-Lu Kung Jung-Lung Tsai Chien-Hsing Lee Kuo-Long Lou Chih-Yung Tang Horng-Huei Liou Kuan-Ling Lu Yi-Hung Chen Wun-Jheng Wang Ming-Cheng Tsai Effects of Sodium Azide, Barium Ion, d-Amphetamine and Procaine on Inward Rectifying Potassium Channel 6.2 Expressed in Xenopus Oocytes Journal of the Formosan Medical Association amphetamine barium ion inward rectifying potassium channel Kir6.2 KATP mRNA procaine sodium azide Xenopus oocyte |
| title | Effects of Sodium Azide, Barium Ion, d-Amphetamine and Procaine on Inward Rectifying Potassium Channel 6.2 Expressed in Xenopus Oocytes |
| title_full | Effects of Sodium Azide, Barium Ion, d-Amphetamine and Procaine on Inward Rectifying Potassium Channel 6.2 Expressed in Xenopus Oocytes |
| title_fullStr | Effects of Sodium Azide, Barium Ion, d-Amphetamine and Procaine on Inward Rectifying Potassium Channel 6.2 Expressed in Xenopus Oocytes |
| title_full_unstemmed | Effects of Sodium Azide, Barium Ion, d-Amphetamine and Procaine on Inward Rectifying Potassium Channel 6.2 Expressed in Xenopus Oocytes |
| title_short | Effects of Sodium Azide, Barium Ion, d-Amphetamine and Procaine on Inward Rectifying Potassium Channel 6.2 Expressed in Xenopus Oocytes |
| title_sort | effects of sodium azide barium ion d amphetamine and procaine on inward rectifying potassium channel 6 2 expressed in xenopus oocytes |
| topic | amphetamine barium ion inward rectifying potassium channel Kir6.2 KATP mRNA procaine sodium azide Xenopus oocyte |
| url | http://www.sciencedirect.com/science/article/pii/S0929664608601771 |
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