In-vitro micrografting technique in sweet orange (Citrus sinensis) cv. Blood Red to produce virus free plants
This study was carried out to assess the potential use and applicability of micrografting technique for the development of virus free nursery in sweet orange (Citrus sinensis Osbeck) cv. Blood Red Malta. The micro grafting was carried out using 11-20 days old etiolated seedlings of Rough Lemon, Sou...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2019-03-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/87595 |
| _version_ | 1797896988195291136 |
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| author | JYOTI KANWAR M K KAUL I S NARUKA P P SINGH |
| author_facet | JYOTI KANWAR M K KAUL I S NARUKA P P SINGH |
| author_sort | JYOTI KANWAR |
| collection | DOAJ |
| description |
This study was carried out to assess the potential use and applicability of micrografting technique for the development of virus free nursery in sweet orange (Citrus sinensis Osbeck) cv. Blood Red Malta. The micro grafting was carried out using 11-20 days old etiolated seedlings of Rough Lemon, Sour orange, Carrizo and Karna Khatta rootstocks. An apical meristem (1-2 mm) having 3-leaf primordial (0.3-0.5 mm) were taken from the axillary proliferated nodal segments. Finely excised meristems were placed on aseptically grown rootstocks making an inverted 'T' incision in vitro. Micro grafts were inoculated on MS media added with different Plant Growth Regulators, i.e. IAA, NAA and 2,4-D each under varied concentrations (control, 0.1 ppm, 0.5 ppm, 1.0 ppm , 1.5 ppm, 2.0 and 3.0 ppm). Highest grafts success (60%) was recorded when 13 days old Carrizo seedlings were used as rootstock. Among all the treatments, scion treated with 2,4-D at 3.0 mg/l exerted maximum positive effect on the success of STG (70.00%). Overall, Sweet orange cv. Blood Red, micro grafted on Carrizo rootstock culturing on liquid MS media containing 2, 4-D at 3.0 mg/L resulted maximum success and growth of grafts. Successful micrografts were hardened under controlled conditions and further shifted in insect proof net house to develop disease free mother progeny.
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| first_indexed | 2024-04-10T07:50:27Z |
| format | Article |
| id | doaj.art-f4c11581371f462baaae2871effc4854 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T07:50:27Z |
| publishDate | 2019-03-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-f4c11581371f462baaae2871effc48542023-02-23T10:18:43ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192019-03-0189310.56093/ijas.v89i3.87595In-vitro micrografting technique in sweet orange (Citrus sinensis) cv. Blood Red to produce virus free plantsJYOTI KANWAR0M K KAUL1I S NARUKA2P P SINGH3Ph D Scholar, SKRAU College of Agriculture, Bikaner, Rajasthan 334 001Former Professor, SKRAU College of Agriculture, Bikaner, Rajasthan 334 001Professor, AU, Jodhpur, RajasthanPrincipal Scientist, CIAH, Bikaner This study was carried out to assess the potential use and applicability of micrografting technique for the development of virus free nursery in sweet orange (Citrus sinensis Osbeck) cv. Blood Red Malta. The micro grafting was carried out using 11-20 days old etiolated seedlings of Rough Lemon, Sour orange, Carrizo and Karna Khatta rootstocks. An apical meristem (1-2 mm) having 3-leaf primordial (0.3-0.5 mm) were taken from the axillary proliferated nodal segments. Finely excised meristems were placed on aseptically grown rootstocks making an inverted 'T' incision in vitro. Micro grafts were inoculated on MS media added with different Plant Growth Regulators, i.e. IAA, NAA and 2,4-D each under varied concentrations (control, 0.1 ppm, 0.5 ppm, 1.0 ppm , 1.5 ppm, 2.0 and 3.0 ppm). Highest grafts success (60%) was recorded when 13 days old Carrizo seedlings were used as rootstock. Among all the treatments, scion treated with 2,4-D at 3.0 mg/l exerted maximum positive effect on the success of STG (70.00%). Overall, Sweet orange cv. Blood Red, micro grafted on Carrizo rootstock culturing on liquid MS media containing 2, 4-D at 3.0 mg/L resulted maximum success and growth of grafts. Successful micrografts were hardened under controlled conditions and further shifted in insect proof net house to develop disease free mother progeny. https://epubs.icar.org.in/index.php/IJAgS/article/view/87595Blood RedCitrus aurantiumC. carrizoC. jambheriC. karnaMicrografting |
| spellingShingle | JYOTI KANWAR M K KAUL I S NARUKA P P SINGH In-vitro micrografting technique in sweet orange (Citrus sinensis) cv. Blood Red to produce virus free plants The Indian Journal of Agricultural Sciences Blood Red Citrus aurantium C. carrizo C. jambheri C. karna Micrografting |
| title | In-vitro micrografting technique in sweet orange (Citrus sinensis) cv. Blood Red to produce virus free plants |
| title_full | In-vitro micrografting technique in sweet orange (Citrus sinensis) cv. Blood Red to produce virus free plants |
| title_fullStr | In-vitro micrografting technique in sweet orange (Citrus sinensis) cv. Blood Red to produce virus free plants |
| title_full_unstemmed | In-vitro micrografting technique in sweet orange (Citrus sinensis) cv. Blood Red to produce virus free plants |
| title_short | In-vitro micrografting technique in sweet orange (Citrus sinensis) cv. Blood Red to produce virus free plants |
| title_sort | in vitro micrografting technique in sweet orange citrus sinensis cv blood red to produce virus free plants |
| topic | Blood Red Citrus aurantium C. carrizo C. jambheri C. karna Micrografting |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/87595 |
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