Euchromatic histone lysine methyltransferase 2 facilitates radioresistance in prostate cancer by repressing endoplasmic reticulum protein 29 transcription
Abstract Prostate cancer is one of the most common cancers in men. This study was conducted to investigate the role of euchromatic histone lysine methyltransferase 2 (EHMT2) and endoplasmic reticulum protein 29 (ERP29) in the progression of radioresistance in prostate cancer. The expression of EHMT2...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | Kaohsiung Journal of Medical Sciences |
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| Online Access: | https://doi.org/10.1002/kjm2.12661 |
| _version_ | 1797808853357690880 |
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| author | Zhi‐Chao Huang Jun Huang Chang‐Kun Huang Yi Hou Bin Zhu |
| author_facet | Zhi‐Chao Huang Jun Huang Chang‐Kun Huang Yi Hou Bin Zhu |
| author_sort | Zhi‐Chao Huang |
| collection | DOAJ |
| description | Abstract Prostate cancer is one of the most common cancers in men. This study was conducted to investigate the role of euchromatic histone lysine methyltransferase 2 (EHMT2) and endoplasmic reticulum protein 29 (ERP29) in the progression of radioresistance in prostate cancer. The expression of EHMT2 and ERP29 in prostate cancer cells and during the progression of radioresistance was detected using quantitative reverse transcription‐polymerase chain reaction and western blotting, and the interaction between EHMT2 and ERP29 was investigated. The proliferation of transfected cells under x‐ray irradiation was determined using the methyl thiazolyl tetrazolium and colony formation assays. Flow cytometry was used to analyze the apoptosis of the transfected cells under x‐ray irradiation. Nude mice were subcutaneously injected with prostate cancer (DU145) cells stably transfected with sh‐ERP29 or sh‐NC. The effect of ERP29 expression on radioresistance in nude mice was assessed by x‐ray irradiation. The expression of EHMT2 was upregulated and that of ERP29 was downregulated in prostate cancer cells during radioresistance progression. EHMT2 downregulation suppressed radioresistance in DU145 and androgen‐sensitive prostate cancer (LNCaP) cells. In irradiated DU145 cells, EHMT2 inhibition decreased the number of colonies and accelerated apoptosis. The transcription of ERP29 was suppressed by EHMT2 by upregulating H3K9me2 and downregulating H3K4me3, thereby regulating radioresistance in prostate cancer cells. In addition, the downregulation of ERP29 promoted the progression of radioresistance in prostate cancer cells in nude mice. EHMT2 promotes radioresistance in prostate cancer cells by repressing ERP29 transcription. |
| first_indexed | 2024-03-13T06:43:50Z |
| format | Article |
| id | doaj.art-f4c2d677d0a74c97a7138e62229c5289 |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-03-13T06:43:50Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-f4c2d677d0a74c97a7138e62229c52892023-06-08T13:32:18ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502023-06-0139657658610.1002/kjm2.12661Euchromatic histone lysine methyltransferase 2 facilitates radioresistance in prostate cancer by repressing endoplasmic reticulum protein 29 transcriptionZhi‐Chao Huang0Jun Huang1Chang‐Kun Huang2Yi Hou3Bin Zhu4Department of Urology Second Xiangya Hospital, Central South University Changsha Hunan People's Republic of ChinaDepartment of Urology Second Xiangya Hospital, Central South University Changsha Hunan People's Republic of ChinaDepartment of Urology Second Xiangya Hospital, Central South University Changsha Hunan People's Republic of ChinaDepartment of Urology Second Xiangya Hospital, Central South University Changsha Hunan People's Republic of ChinaDepartment of Urology Second Xiangya Hospital, Central South University Changsha Hunan People's Republic of ChinaAbstract Prostate cancer is one of the most common cancers in men. This study was conducted to investigate the role of euchromatic histone lysine methyltransferase 2 (EHMT2) and endoplasmic reticulum protein 29 (ERP29) in the progression of radioresistance in prostate cancer. The expression of EHMT2 and ERP29 in prostate cancer cells and during the progression of radioresistance was detected using quantitative reverse transcription‐polymerase chain reaction and western blotting, and the interaction between EHMT2 and ERP29 was investigated. The proliferation of transfected cells under x‐ray irradiation was determined using the methyl thiazolyl tetrazolium and colony formation assays. Flow cytometry was used to analyze the apoptosis of the transfected cells under x‐ray irradiation. Nude mice were subcutaneously injected with prostate cancer (DU145) cells stably transfected with sh‐ERP29 or sh‐NC. The effect of ERP29 expression on radioresistance in nude mice was assessed by x‐ray irradiation. The expression of EHMT2 was upregulated and that of ERP29 was downregulated in prostate cancer cells during radioresistance progression. EHMT2 downregulation suppressed radioresistance in DU145 and androgen‐sensitive prostate cancer (LNCaP) cells. In irradiated DU145 cells, EHMT2 inhibition decreased the number of colonies and accelerated apoptosis. The transcription of ERP29 was suppressed by EHMT2 by upregulating H3K9me2 and downregulating H3K4me3, thereby regulating radioresistance in prostate cancer cells. In addition, the downregulation of ERP29 promoted the progression of radioresistance in prostate cancer cells in nude mice. EHMT2 promotes radioresistance in prostate cancer cells by repressing ERP29 transcription.https://doi.org/10.1002/kjm2.12661EHMT2ERP29histone methylationprostate cancerradioresistance |
| spellingShingle | Zhi‐Chao Huang Jun Huang Chang‐Kun Huang Yi Hou Bin Zhu Euchromatic histone lysine methyltransferase 2 facilitates radioresistance in prostate cancer by repressing endoplasmic reticulum protein 29 transcription Kaohsiung Journal of Medical Sciences EHMT2 ERP29 histone methylation prostate cancer radioresistance |
| title | Euchromatic histone lysine methyltransferase 2 facilitates radioresistance in prostate cancer by repressing endoplasmic reticulum protein 29 transcription |
| title_full | Euchromatic histone lysine methyltransferase 2 facilitates radioresistance in prostate cancer by repressing endoplasmic reticulum protein 29 transcription |
| title_fullStr | Euchromatic histone lysine methyltransferase 2 facilitates radioresistance in prostate cancer by repressing endoplasmic reticulum protein 29 transcription |
| title_full_unstemmed | Euchromatic histone lysine methyltransferase 2 facilitates radioresistance in prostate cancer by repressing endoplasmic reticulum protein 29 transcription |
| title_short | Euchromatic histone lysine methyltransferase 2 facilitates radioresistance in prostate cancer by repressing endoplasmic reticulum protein 29 transcription |
| title_sort | euchromatic histone lysine methyltransferase 2 facilitates radioresistance in prostate cancer by repressing endoplasmic reticulum protein 29 transcription |
| topic | EHMT2 ERP29 histone methylation prostate cancer radioresistance |
| url | https://doi.org/10.1002/kjm2.12661 |
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