ONT read assembly of the black rhino genome
Abstract Objectives The black rhinoceros (Diceros bicornis) is an endangered mammal for which a captive breeding program is part of the conservation effort. Black rhinos in zoo’s often suffer from chronic infections and heamochromatosis. Furthermore, breeding is hampered by low male fertility. To ai...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-03-01
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| Series: | BMC Genomic Data |
| Online Access: | https://doi.org/10.1186/s12863-024-01214-0 |
| _version_ | 1827326069850505216 |
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| author | Ken Kraaijeveld Koen Bossers Nikola Petrusevski Stef Pieterman Linda G.R. Bruins-van Sonsbeek Floyd Wittink |
| author_facet | Ken Kraaijeveld Koen Bossers Nikola Petrusevski Stef Pieterman Linda G.R. Bruins-van Sonsbeek Floyd Wittink |
| author_sort | Ken Kraaijeveld |
| collection | DOAJ |
| description | Abstract Objectives The black rhinoceros (Diceros bicornis) is an endangered mammal for which a captive breeding program is part of the conservation effort. Black rhinos in zoo’s often suffer from chronic infections and heamochromatosis. Furthermore, breeding is hampered by low male fertility. To aid a research project studying these topics, we sequenced and assembled the genome of a captive male black rhino using ONT sequencing data only. Data description This work produced over 100 Gb whole genome sequencing reads from whole blood. These were assembled into a 2.47 Gb draft genome consisting of 834 contigs with an N50 of 29.53 Mb. The genome annotation was lifted over from an available genome annotation for black rhino, which resulted in the retrieval of over 99% of gene features. This new genome assembly will be a valuable resource in for conservation genetic research in this species. Keywords Black rhinoceros, Diceros bicornis, Genome assembly, Long reads, Whole genome sequencing. |
| first_indexed | 2024-03-07T14:39:19Z |
| format | Article |
| id | doaj.art-f4c6ce5a09f9488280aeb5e4b8c24670 |
| institution | Directory Open Access Journal |
| issn | 2730-6844 |
| language | English |
| last_indexed | 2024-03-07T14:39:19Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomic Data |
| spelling | doaj.art-f4c6ce5a09f9488280aeb5e4b8c246702024-03-05T20:29:45ZengBMCBMC Genomic Data2730-68442024-03-012511210.1186/s12863-024-01214-0ONT read assembly of the black rhino genomeKen Kraaijeveld0Koen Bossers1Nikola Petrusevski2Stef Pieterman3Linda G.R. Bruins-van Sonsbeek4Floyd Wittink5Leiden Centre for Applied Bioscience, University of Applied Sciences LeidenLeiden Centre for Applied Bioscience, University of Applied Sciences LeidenLeiden Centre for Applied Bioscience, University of Applied Sciences LeidenLeiden Centre for Applied Bioscience, University of Applied Sciences LeidenRotterdam ZooLeiden Centre for Applied Bioscience, University of Applied Sciences LeidenAbstract Objectives The black rhinoceros (Diceros bicornis) is an endangered mammal for which a captive breeding program is part of the conservation effort. Black rhinos in zoo’s often suffer from chronic infections and heamochromatosis. Furthermore, breeding is hampered by low male fertility. To aid a research project studying these topics, we sequenced and assembled the genome of a captive male black rhino using ONT sequencing data only. Data description This work produced over 100 Gb whole genome sequencing reads from whole blood. These were assembled into a 2.47 Gb draft genome consisting of 834 contigs with an N50 of 29.53 Mb. The genome annotation was lifted over from an available genome annotation for black rhino, which resulted in the retrieval of over 99% of gene features. This new genome assembly will be a valuable resource in for conservation genetic research in this species. Keywords Black rhinoceros, Diceros bicornis, Genome assembly, Long reads, Whole genome sequencing.https://doi.org/10.1186/s12863-024-01214-0 |
| spellingShingle | Ken Kraaijeveld Koen Bossers Nikola Petrusevski Stef Pieterman Linda G.R. Bruins-van Sonsbeek Floyd Wittink ONT read assembly of the black rhino genome BMC Genomic Data |
| title | ONT read assembly of the black rhino genome |
| title_full | ONT read assembly of the black rhino genome |
| title_fullStr | ONT read assembly of the black rhino genome |
| title_full_unstemmed | ONT read assembly of the black rhino genome |
| title_short | ONT read assembly of the black rhino genome |
| title_sort | ont read assembly of the black rhino genome |
| url | https://doi.org/10.1186/s12863-024-01214-0 |
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