Association of IL-4 (− 590 C/T) and IL-6 (− 174 G/C) gene polymorphism in South Indian CKD patients
Abstract Aim The present study was undertaken to examine the role of IL-4 (− 590 C/T) (rs2243250) and IL-6 (− 174G/C) (rs1800795) polymorphism and the serum levels of IL-4 and IL-6 in chronic kidney disease (CKD). Methods The IL-4 (− 590C/T) and IL-6 (− 174 G/C) polymorphisms were genotyped in 132 C...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2024-02-01
|
| Series: | Egyptian Journal of Medical Human Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43042-024-00476-8 |
| _version_ | 1797274413813465088 |
|---|---|
| author | Vandit Sevak Rathika Chinniah Sasiharan Pandi K. Sampathkumar T. Dinakaran Balakrishnan Karuppiah |
| author_facet | Vandit Sevak Rathika Chinniah Sasiharan Pandi K. Sampathkumar T. Dinakaran Balakrishnan Karuppiah |
| author_sort | Vandit Sevak |
| collection | DOAJ |
| description | Abstract Aim The present study was undertaken to examine the role of IL-4 (− 590 C/T) (rs2243250) and IL-6 (− 174G/C) (rs1800795) polymorphism and the serum levels of IL-4 and IL-6 in chronic kidney disease (CKD). Methods The IL-4 (− 590C/T) and IL-6 (− 174 G/C) polymorphisms were genotyped in 132 CKD patients and 161 controls using PCR–RFLP. Serum IL-4 and IL-6 quantifications were performed by ELISA. Results Significant susceptible associations of CT genotype (OR = 4.56; p < 1.84 × 10–9) and T allele (OR = 1.56; p < 0.010) of IL-4 (− 590C/T) and CC genotype (OR = 2.63; p < 0.032) of IL-6 (− 174G/C) were observed for CKD. The CC genotype (OR = 0.27; p < 9.314 × 10–7) and C allele (OR = 0.63; p < 0.010) of IL-4 (− 590 C/T) revealed strong protective associations. Five-fold increased levels were observed for both IL-6 (p < 0.0001) and IL-4 (p < 0.0043) cytokines in CKD patients than the controls. The IL-4 serum levels (pg/ml) increased significantly in patients with CT and TT genotypes of IL-4 (− 590 C/T) than the controls (6.18 ± 1.80 vs. 3.33 ± 0.48 and 6.14 ± 1.96 vs. 3.21 ± 0.56 respectively). For IL-6 (− 174 G/C) polymorphism, the patients with CC genotype (6.50 ± 1.30 vs. 3.49 ± 1.39) revealed with higher IL-6 serum levels followed by GC genotype (5.00 ± 1.91 vs. 4.01 ± 1.74). Conclusion The genotypes of IL-4 (590 C/T) and IL-6 (174 G/C) polymorphisms contribute differential susceptibility in south Indian CKD patients. A fivefold increased serum levels of IL-4 (anti-inflammatory) and IL-6 (pro- and anti-inflammatory) cytokines were documented in CKD patients. There observed an opposite trend in disease association for these two cytokines and associated SNPs with CKD in south India. |
| first_indexed | 2024-03-07T14:57:58Z |
| format | Article |
| id | doaj.art-f4c751e586f441da8703f5e002287baf |
| institution | Directory Open Access Journal |
| issn | 2090-2441 |
| language | English |
| last_indexed | 2024-03-07T14:57:58Z |
| publishDate | 2024-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj.art-f4c751e586f441da8703f5e002287baf2024-03-05T19:19:22ZengSpringerOpenEgyptian Journal of Medical Human Genetics2090-24412024-02-0125111010.1186/s43042-024-00476-8Association of IL-4 (− 590 C/T) and IL-6 (− 174 G/C) gene polymorphism in South Indian CKD patientsVandit Sevak0Rathika Chinniah1Sasiharan Pandi2K. Sampathkumar3T. Dinakaran4Balakrishnan Karuppiah5Department of Immunology, Madurai Kamaraj UniversityMadurai Kidney Centre and Transplantation Research Institute (MKCTRI)Department of Immunology, Madurai Kamaraj UniversityMeenakshi Mission Hospital and Research InstituteMadurai Kidney Centre and Transplantation Research Institute (MKCTRI)Madurai Kidney Centre and Transplantation Research Institute (MKCTRI)Abstract Aim The present study was undertaken to examine the role of IL-4 (− 590 C/T) (rs2243250) and IL-6 (− 174G/C) (rs1800795) polymorphism and the serum levels of IL-4 and IL-6 in chronic kidney disease (CKD). Methods The IL-4 (− 590C/T) and IL-6 (− 174 G/C) polymorphisms were genotyped in 132 CKD patients and 161 controls using PCR–RFLP. Serum IL-4 and IL-6 quantifications were performed by ELISA. Results Significant susceptible associations of CT genotype (OR = 4.56; p < 1.84 × 10–9) and T allele (OR = 1.56; p < 0.010) of IL-4 (− 590C/T) and CC genotype (OR = 2.63; p < 0.032) of IL-6 (− 174G/C) were observed for CKD. The CC genotype (OR = 0.27; p < 9.314 × 10–7) and C allele (OR = 0.63; p < 0.010) of IL-4 (− 590 C/T) revealed strong protective associations. Five-fold increased levels were observed for both IL-6 (p < 0.0001) and IL-4 (p < 0.0043) cytokines in CKD patients than the controls. The IL-4 serum levels (pg/ml) increased significantly in patients with CT and TT genotypes of IL-4 (− 590 C/T) than the controls (6.18 ± 1.80 vs. 3.33 ± 0.48 and 6.14 ± 1.96 vs. 3.21 ± 0.56 respectively). For IL-6 (− 174 G/C) polymorphism, the patients with CC genotype (6.50 ± 1.30 vs. 3.49 ± 1.39) revealed with higher IL-6 serum levels followed by GC genotype (5.00 ± 1.91 vs. 4.01 ± 1.74). Conclusion The genotypes of IL-4 (590 C/T) and IL-6 (174 G/C) polymorphisms contribute differential susceptibility in south Indian CKD patients. A fivefold increased serum levels of IL-4 (anti-inflammatory) and IL-6 (pro- and anti-inflammatory) cytokines were documented in CKD patients. There observed an opposite trend in disease association for these two cytokines and associated SNPs with CKD in south India.https://doi.org/10.1186/s43042-024-00476-8CytokinesIL-4IL-6Chronic kidney diseaseInflammationCytokine dynamics |
| spellingShingle | Vandit Sevak Rathika Chinniah Sasiharan Pandi K. Sampathkumar T. Dinakaran Balakrishnan Karuppiah Association of IL-4 (− 590 C/T) and IL-6 (− 174 G/C) gene polymorphism in South Indian CKD patients Egyptian Journal of Medical Human Genetics Cytokines IL-4 IL-6 Chronic kidney disease Inflammation Cytokine dynamics |
| title | Association of IL-4 (− 590 C/T) and IL-6 (− 174 G/C) gene polymorphism in South Indian CKD patients |
| title_full | Association of IL-4 (− 590 C/T) and IL-6 (− 174 G/C) gene polymorphism in South Indian CKD patients |
| title_fullStr | Association of IL-4 (− 590 C/T) and IL-6 (− 174 G/C) gene polymorphism in South Indian CKD patients |
| title_full_unstemmed | Association of IL-4 (− 590 C/T) and IL-6 (− 174 G/C) gene polymorphism in South Indian CKD patients |
| title_short | Association of IL-4 (− 590 C/T) and IL-6 (− 174 G/C) gene polymorphism in South Indian CKD patients |
| title_sort | association of il 4 590 c t and il 6 174 g c gene polymorphism in south indian ckd patients |
| topic | Cytokines IL-4 IL-6 Chronic kidney disease Inflammation Cytokine dynamics |
| url | https://doi.org/10.1186/s43042-024-00476-8 |
| work_keys_str_mv | AT vanditsevak associationofil4590ctandil6174gcgenepolymorphisminsouthindianckdpatients AT rathikachinniah associationofil4590ctandil6174gcgenepolymorphisminsouthindianckdpatients AT sasiharanpandi associationofil4590ctandil6174gcgenepolymorphisminsouthindianckdpatients AT ksampathkumar associationofil4590ctandil6174gcgenepolymorphisminsouthindianckdpatients AT tdinakaran associationofil4590ctandil6174gcgenepolymorphisminsouthindianckdpatients AT balakrishnankaruppiah associationofil4590ctandil6174gcgenepolymorphisminsouthindianckdpatients |