A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1

Abstract Virus-induced gene silencing (VIGS) is a powerful technology for molecular characterization of gene functions in plants and has the potential to prevent and control plant diseases. Unfortunately, VIGS in many woody perennials such as citrus are severely hindered because they have a long juv...

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Main Authors: Chunqing Wang, Zhimin Ma, Jinhuan Zhou, Jiaxin Li, Peng Cao, Chenhu Song, Chuxin Li, Xinliang Wang, Yu Bin, Changyong Zhou, Zhen Song
Format: Article
Language:English
Published: BMC 2024-03-01
Series:Phytopathology Research
Subjects:
Online Access:https://doi.org/10.1186/s42483-024-00234-z
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author Chunqing Wang
Zhimin Ma
Jinhuan Zhou
Jiaxin Li
Peng Cao
Chenhu Song
Chuxin Li
Xinliang Wang
Yu Bin
Changyong Zhou
Zhen Song
author_facet Chunqing Wang
Zhimin Ma
Jinhuan Zhou
Jiaxin Li
Peng Cao
Chenhu Song
Chuxin Li
Xinliang Wang
Yu Bin
Changyong Zhou
Zhen Song
author_sort Chunqing Wang
collection DOAJ
description Abstract Virus-induced gene silencing (VIGS) is a powerful technology for molecular characterization of gene functions in plants and has the potential to prevent and control plant diseases. Unfortunately, VIGS in many woody perennials such as citrus are severely hindered because they have a long juvenile period and are recalcitrant to infection compared to herbaceous plants. Here, we report the development of a simple and effective VIGS system based on citrus leaf blotch virus (CLBV), a virus endemic in most citrus-producing countries but showing no symptoms in most citrus varieties. By Agrobacterium-mediated vacuum-infiltration (AVI), pCLBV201-su245 and pCLBV201-pds391 triggered efficient gene silencing (85%) and silencing phenotypes began to appear at 30 days post infiltration/inoculation (dpi), a similar period for achieving that on herbaceous plants. Moreover, the silencing phenotypes could still be observed four years post inoculation. Further, pCLBV201-lob369 was constructed to silence the gene lateral organ boundaries1 (lob1), encoding a key factor for susceptibility to citrus canker that caused by Xanthomonas citri ssp. citri (Xcc). The resistance evaluation results showed that the lesion area, disease index, and bacterial content in the pCLBV201-lob369 treatment group were decreased by 64%, 14%, and 67%, respectively. This work provides a simple and effective VIGS system for citrus which has the potential to be used for diseases control.
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spelling doaj.art-f4d1b6be3a4745b0bfb5eade656a59512024-03-17T12:17:13ZengBMCPhytopathology Research2524-41672024-03-016111210.1186/s42483-024-00234-zA simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1Chunqing Wang0Zhimin Ma1Jinhuan Zhou2Jiaxin Li3Peng Cao4Chenhu Song5Chuxin Li6Xinliang Wang7Yu Bin8Changyong Zhou9Zhen Song10Citrus Research Institute, Southwest University/National Citrus Engineering Research CenterCitrus Research Institute, Southwest University/National Citrus Engineering Research CenterCitrus Research Institute, Southwest University/National Citrus Engineering Research CenterCitrus Research Institute, Southwest University/National Citrus Engineering Research CenterCitrus Research Institute, Southwest University/National Citrus Engineering Research CenterCitrus Research Institute, Southwest University/National Citrus Engineering Research CenterCitrus Research Institute, Southwest University/National Citrus Engineering Research CenterCitrus Research Institute, Southwest University/National Citrus Engineering Research CenterCitrus Research Institute, Southwest University/National Citrus Engineering Research CenterCitrus Research Institute, Southwest University/National Citrus Engineering Research CenterCitrus Research Institute, Southwest University/National Citrus Engineering Research CenterAbstract Virus-induced gene silencing (VIGS) is a powerful technology for molecular characterization of gene functions in plants and has the potential to prevent and control plant diseases. Unfortunately, VIGS in many woody perennials such as citrus are severely hindered because they have a long juvenile period and are recalcitrant to infection compared to herbaceous plants. Here, we report the development of a simple and effective VIGS system based on citrus leaf blotch virus (CLBV), a virus endemic in most citrus-producing countries but showing no symptoms in most citrus varieties. By Agrobacterium-mediated vacuum-infiltration (AVI), pCLBV201-su245 and pCLBV201-pds391 triggered efficient gene silencing (85%) and silencing phenotypes began to appear at 30 days post infiltration/inoculation (dpi), a similar period for achieving that on herbaceous plants. Moreover, the silencing phenotypes could still be observed four years post inoculation. Further, pCLBV201-lob369 was constructed to silence the gene lateral organ boundaries1 (lob1), encoding a key factor for susceptibility to citrus canker that caused by Xanthomonas citri ssp. citri (Xcc). The resistance evaluation results showed that the lesion area, disease index, and bacterial content in the pCLBV201-lob369 treatment group were decreased by 64%, 14%, and 67%, respectively. This work provides a simple and effective VIGS system for citrus which has the potential to be used for diseases control.https://doi.org/10.1186/s42483-024-00234-zCitrus leaf blotch virus (CLBV)Virus-induced gene silencing (VIGS)Citrus cankerDisease control
spellingShingle Chunqing Wang
Zhimin Ma
Jinhuan Zhou
Jiaxin Li
Peng Cao
Chenhu Song
Chuxin Li
Xinliang Wang
Yu Bin
Changyong Zhou
Zhen Song
A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1
Phytopathology Research
Citrus leaf blotch virus (CLBV)
Virus-induced gene silencing (VIGS)
Citrus canker
Disease control
title A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1
title_full A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1
title_fullStr A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1
title_full_unstemmed A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1
title_short A simple and effective VIGS system facilitates the control of citrus canker by silencing CsLOB1
title_sort simple and effective vigs system facilitates the control of citrus canker by silencing cslob1
topic Citrus leaf blotch virus (CLBV)
Virus-induced gene silencing (VIGS)
Citrus canker
Disease control
url https://doi.org/10.1186/s42483-024-00234-z
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