Development of method for the mineral water catalase activity determination

Biological effects of mineral water depend not only on the chemical composition but also on the metabolic products of microbial cenosis. Among numerous microorganisms constituting the autochthonous microflora of mineral waters, we do evolve the saprophytic organisms producing the catalase, the sapro...

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Main Authors: Olena М. Nikipelova, Аlena Yu. Kisilevskaya, Lyudmyla B. Solodova
Format: Article
Language:English
Published: Odessa National Polytechnic University 2015-03-01
Series:Trudy Odesskogo Politehničeskogo Universiteta
Subjects:
Online Access:http://pratsi.opu.ua/articles/show/1164
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author Olena М. Nikipelova
Аlena Yu. Kisilevskaya
Lyudmyla B. Solodova
author_facet Olena М. Nikipelova
Аlena Yu. Kisilevskaya
Lyudmyla B. Solodova
author_sort Olena М. Nikipelova
collection DOAJ
description Biological effects of mineral water depend not only on the chemical composition but also on the metabolic products of microbial cenosis. Among numerous microorganisms constituting the autochthonous microflora of mineral waters, we do evolve the saprophytic organisms producing the catalase, the saprophytes’ physiological and biological role being proven a long ago. The research aim was to develop a method for determination of mineral water catalase activity. Analyzed are various methods to determine the catalase activity in biological objects. Developed is a spectrophotometric method for determination of mineral water catalase activity. The method is efficiently tested with series of Ukrainian mineral waters. Calculated are the relative standard deviations which are significantly below normal errors, admitted at spectroscopic analysis and at the optic density range. The given method provides sufficient accuracy and convergence when estimating the mineral waters catalase activity, allowing to introduce a new index to assess the quality and biological value.
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spelling doaj.art-f4d1bdc761db421d839d871e2734d61d2022-12-22T00:05:28ZengOdessa National Polytechnic UniversityTrudy Odesskogo Politehničeskogo Universiteta2076-24292223-38142015-03-012015117618010.15276/opu.1.45.2015.29Development of method for the mineral water catalase activity determinationOlena М. Nikipelova0Аlena Yu. Kisilevskaya1Lyudmyla B. Solodova2Ukrainian Research Institute of Medical Rehabilitation and Balneology of Ministry of Health of UkraineUkrainian Research Institute of Medical Rehabilitation and Balneology of Ministry of Health of UkraineUkrainian Research Institute of Medical Rehabilitation and Balneology of Ministry of Health of UkraineBiological effects of mineral water depend not only on the chemical composition but also on the metabolic products of microbial cenosis. Among numerous microorganisms constituting the autochthonous microflora of mineral waters, we do evolve the saprophytic organisms producing the catalase, the saprophytes’ physiological and biological role being proven a long ago. The research aim was to develop a method for determination of mineral water catalase activity. Analyzed are various methods to determine the catalase activity in biological objects. Developed is a spectrophotometric method for determination of mineral water catalase activity. The method is efficiently tested with series of Ukrainian mineral waters. Calculated are the relative standard deviations which are significantly below normal errors, admitted at spectroscopic analysis and at the optic density range. The given method provides sufficient accuracy and convergence when estimating the mineral waters catalase activity, allowing to introduce a new index to assess the quality and biological value.http://pratsi.opu.ua/articles/show/1164catalase activitymineral watersspectrophotometric methodsaprophytic microorganisms
spellingShingle Olena М. Nikipelova
Аlena Yu. Kisilevskaya
Lyudmyla B. Solodova
Development of method for the mineral water catalase activity determination
Trudy Odesskogo Politehničeskogo Universiteta
catalase activity
mineral waters
spectrophotometric method
saprophytic microorganisms
title Development of method for the mineral water catalase activity determination
title_full Development of method for the mineral water catalase activity determination
title_fullStr Development of method for the mineral water catalase activity determination
title_full_unstemmed Development of method for the mineral water catalase activity determination
title_short Development of method for the mineral water catalase activity determination
title_sort development of method for the mineral water catalase activity determination
topic catalase activity
mineral waters
spectrophotometric method
saprophytic microorganisms
url http://pratsi.opu.ua/articles/show/1164
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AT alenayukisilevskaya developmentofmethodforthemineralwatercatalaseactivitydetermination
AT lyudmylabsolodova developmentofmethodforthemineralwatercatalaseactivitydetermination