Over Expression of FOXO1 in Subcutaneous Fatty Tissue and its Response to Resistance Training in High Fat Diet and Type 2 Diabetic Rat
Objective: Forkhead box proteins and Forkhead box transcription factor O1 (FOXO1) in particular, mediate insulin signaling pathways and glucose homeostasis. This study aimed to compare FOXO1 expression in subcutaneous adipose (SA) tissue between obese rats with and without type 2 diabetes (T2D) and...
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Sciences
2022-05-01
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| Series: | Iranian Journal of Diabetes and Obesity |
| Subjects: | |
| Online Access: | http://ijdo.ssu.ac.ir/article-1-708-en.html |
| _version_ | 1828199099660238848 |
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| author | Shahram Soheily Mojtaba Eizadi |
| author_facet | Shahram Soheily Mojtaba Eizadi |
| author_sort | Shahram Soheily |
| collection | DOAJ |
| description | Objective: Forkhead box proteins and Forkhead box transcription factor O1 (FOXO1) in particular, mediate insulin signaling pathways and glucose homeostasis. This study aimed to compare FOXO1 expression in subcutaneous adipose (SA) tissue between obese rats with and without type 2 diabetes (T2D) and its response to resistance training in T2D.
Materials and Methods: 21 male wistar rats (220±20 g) were obese by 6 weeks high fat diet (HFD) and randomly assigned to either non-diabetes (n=7) or two T2D groups (control and exercise groups, n=7 in each case). Fasting glucose, insulin, insulin resistance and FOXO1 expression were compared between non-diabetes and diabetes groups. All variables were also assigned after resistance training in the form of climbing a ladder (6 weeks/5 times weekly) in exercise compare with control groups. Data were compared by ANOVA, independent and paired t-test methods (P<0.05).
Results: Induction of diabetes resulted in significant increase in insulin resistance, glucose, and FOXO expression in SA tissue and a decrease in insulin compared to obese health rats (P< 0.0001). A significant decrease in fasting insulin (P< 0.0001), insulin resistance (P< 0.0001) and FOXO1 expression in SA tissue (P< 0.0001) and increase in insulin (P: 0.002) were observed by resistance training compared to control diabetes rats.
Conclusion: Based on our results, improving insulin resistance and glucose in response to resistance training in obese diabetic rats may be rooted in decreased insulin expression following these exercises. |
| first_indexed | 2024-04-12T10:49:31Z |
| format | Article |
| id | doaj.art-f4d30c84704e4885b79158eb6e9cdb2c |
| institution | Directory Open Access Journal |
| issn | 2008-6792 2345-2250 |
| language | English |
| last_indexed | 2024-04-12T10:49:31Z |
| publishDate | 2022-05-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Diabetes and Obesity |
| spelling | doaj.art-f4d30c84704e4885b79158eb6e9cdb2c2022-12-22T03:36:19ZengShahid Sadoughi University of Medical SciencesIranian Journal of Diabetes and Obesity2008-67922345-22502022-05-01142110116Over Expression of FOXO1 in Subcutaneous Fatty Tissue and its Response to Resistance Training in High Fat Diet and Type 2 Diabetic RatShahram Soheily0Mojtaba Eizadi1 Assistant professor of Exercise Physiology, Safadasht Branch, Islamic Azad University, Tehran, Iran. Assistant professor of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran. Objective: Forkhead box proteins and Forkhead box transcription factor O1 (FOXO1) in particular, mediate insulin signaling pathways and glucose homeostasis. This study aimed to compare FOXO1 expression in subcutaneous adipose (SA) tissue between obese rats with and without type 2 diabetes (T2D) and its response to resistance training in T2D. Materials and Methods: 21 male wistar rats (220±20 g) were obese by 6 weeks high fat diet (HFD) and randomly assigned to either non-diabetes (n=7) or two T2D groups (control and exercise groups, n=7 in each case). Fasting glucose, insulin, insulin resistance and FOXO1 expression were compared between non-diabetes and diabetes groups. All variables were also assigned after resistance training in the form of climbing a ladder (6 weeks/5 times weekly) in exercise compare with control groups. Data were compared by ANOVA, independent and paired t-test methods (P<0.05). Results: Induction of diabetes resulted in significant increase in insulin resistance, glucose, and FOXO expression in SA tissue and a decrease in insulin compared to obese health rats (P< 0.0001). A significant decrease in fasting insulin (P< 0.0001), insulin resistance (P< 0.0001) and FOXO1 expression in SA tissue (P< 0.0001) and increase in insulin (P: 0.002) were observed by resistance training compared to control diabetes rats. Conclusion: Based on our results, improving insulin resistance and glucose in response to resistance training in obese diabetic rats may be rooted in decreased insulin expression following these exercises.http://ijdo.ssu.ac.ir/article-1-708-en.htmladipose tissueexerciseinsulin resistancediabetes |
| spellingShingle | Shahram Soheily Mojtaba Eizadi Over Expression of FOXO1 in Subcutaneous Fatty Tissue and its Response to Resistance Training in High Fat Diet and Type 2 Diabetic Rat Iranian Journal of Diabetes and Obesity adipose tissue exercise insulin resistance diabetes |
| title | Over Expression of FOXO1 in Subcutaneous Fatty Tissue and its Response to Resistance Training in High Fat Diet and Type 2 Diabetic Rat |
| title_full | Over Expression of FOXO1 in Subcutaneous Fatty Tissue and its Response to Resistance Training in High Fat Diet and Type 2 Diabetic Rat |
| title_fullStr | Over Expression of FOXO1 in Subcutaneous Fatty Tissue and its Response to Resistance Training in High Fat Diet and Type 2 Diabetic Rat |
| title_full_unstemmed | Over Expression of FOXO1 in Subcutaneous Fatty Tissue and its Response to Resistance Training in High Fat Diet and Type 2 Diabetic Rat |
| title_short | Over Expression of FOXO1 in Subcutaneous Fatty Tissue and its Response to Resistance Training in High Fat Diet and Type 2 Diabetic Rat |
| title_sort | over expression of foxo1 in subcutaneous fatty tissue and its response to resistance training in high fat diet and type 2 diabetic rat |
| topic | adipose tissue exercise insulin resistance diabetes |
| url | http://ijdo.ssu.ac.ir/article-1-708-en.html |
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