First Report on Nucleolar Organizer Regions (NORs) Polymorphism and Constitutive Heterochromatin of Moonlight Gourami, Trichopodus microlepis (Perciformes, Osphronemidae)
Nucleolar organizer regions (NORs) polymorphism, constitutive heterochromatin and chromosomal analysis of Moonlight gourami, Trichopodus microlepis in Thailand were firstly reported. Specimens were collected from the Chao Phraya and Mekong Basins, Thailand. The mitotic chromosomes were directly prep...
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| Format: | Article |
| Language: | English |
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Firenze University Press
2021-08-01
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| Series: | Caryologia |
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| Online Access: | https://riviste.fupress.net/index.php/caryologia/article/view/775 |
| _version_ | 1818902066258509824 |
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| author | Weerayuth Supiwong |
| author_facet | Weerayuth Supiwong |
| author_sort | Weerayuth Supiwong |
| collection | DOAJ |
| description | Nucleolar organizer regions (NORs) polymorphism, constitutive heterochromatin and chromosomal analysis of Moonlight gourami, Trichopodus microlepis in Thailand were firstly reported. Specimens were collected from the Chao Phraya and Mekong Basins, Thailand. The mitotic chromosomes were directly prepared from kidney tissues of ten males and ten females. Conventional staining, Ag-NOR banding and C- banding techniques were applied to stain the chromosomes. The results shown that the diploid chromosome number of T. microlepis was 2n=46 and the fundamental number (NF) was 46 in both males and females. The karyotype consisted of 46 telocentric chromosomes classifying as 14 large and 32 medium. No heteromorphic sex chromosome was observed in T. microlepis. The results also exhibited that the interstitial nucleolar organizer regions (NORs) were clearly observed at the long arm of the chromosome pair 7. This is the first report on NORs polymorphism in T. microlepis that a heteromorphic NOR type in one female had a single NOR-bearing chromosome of the chromosome pair 7, whereas 10 males and nine females had two NOR-bearing chromosomes of the chromosome pair 7 with a homomorphic NOR type. Constitutive heterochromatins located at all centromeres of all chromosome pairs. The karyotype formula of T. microlepis is 2n (46) = Lt14 + Mt32. |
| first_indexed | 2024-12-19T20:29:44Z |
| format | Article |
| id | doaj.art-f4d83570ee414463a28a43f8579bba96 |
| institution | Directory Open Access Journal |
| issn | 0008-7114 2165-5391 |
| language | English |
| last_indexed | 2024-12-19T20:29:44Z |
| publishDate | 2021-08-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Caryologia |
| spelling | doaj.art-f4d83570ee414463a28a43f8579bba962022-12-21T20:06:46ZengFirenze University PressCaryologia0008-71142165-53912021-08-0110.36253/caryologia-775First Report on Nucleolar Organizer Regions (NORs) Polymorphism and Constitutive Heterochromatin of Moonlight Gourami, Trichopodus microlepis (Perciformes, Osphronemidae)Weerayuth Supiwong0Faculty of Applied Science and Engineering, Khon Kaen University, Nong Khai Campus, Muang District, Nong Khai Province 43000Nucleolar organizer regions (NORs) polymorphism, constitutive heterochromatin and chromosomal analysis of Moonlight gourami, Trichopodus microlepis in Thailand were firstly reported. Specimens were collected from the Chao Phraya and Mekong Basins, Thailand. The mitotic chromosomes were directly prepared from kidney tissues of ten males and ten females. Conventional staining, Ag-NOR banding and C- banding techniques were applied to stain the chromosomes. The results shown that the diploid chromosome number of T. microlepis was 2n=46 and the fundamental number (NF) was 46 in both males and females. The karyotype consisted of 46 telocentric chromosomes classifying as 14 large and 32 medium. No heteromorphic sex chromosome was observed in T. microlepis. The results also exhibited that the interstitial nucleolar organizer regions (NORs) were clearly observed at the long arm of the chromosome pair 7. This is the first report on NORs polymorphism in T. microlepis that a heteromorphic NOR type in one female had a single NOR-bearing chromosome of the chromosome pair 7, whereas 10 males and nine females had two NOR-bearing chromosomes of the chromosome pair 7 with a homomorphic NOR type. Constitutive heterochromatins located at all centromeres of all chromosome pairs. The karyotype formula of T. microlepis is 2n (46) = Lt14 + Mt32.https://riviste.fupress.net/index.php/caryologia/article/view/775Moonlight gourami, Trichopodus microlepis, Karyotype,,Nucleolar Organizer Region, Constitutive heterochromatin, Chromosome |
| spellingShingle | Weerayuth Supiwong First Report on Nucleolar Organizer Regions (NORs) Polymorphism and Constitutive Heterochromatin of Moonlight Gourami, Trichopodus microlepis (Perciformes, Osphronemidae) Caryologia Moonlight gourami, Trichopodus microlepis, Karyotype,,Nucleolar Organizer Region, Constitutive heterochromatin, Chromosome |
| title | First Report on Nucleolar Organizer Regions (NORs) Polymorphism and Constitutive Heterochromatin of Moonlight Gourami, Trichopodus microlepis (Perciformes, Osphronemidae) |
| title_full | First Report on Nucleolar Organizer Regions (NORs) Polymorphism and Constitutive Heterochromatin of Moonlight Gourami, Trichopodus microlepis (Perciformes, Osphronemidae) |
| title_fullStr | First Report on Nucleolar Organizer Regions (NORs) Polymorphism and Constitutive Heterochromatin of Moonlight Gourami, Trichopodus microlepis (Perciformes, Osphronemidae) |
| title_full_unstemmed | First Report on Nucleolar Organizer Regions (NORs) Polymorphism and Constitutive Heterochromatin of Moonlight Gourami, Trichopodus microlepis (Perciformes, Osphronemidae) |
| title_short | First Report on Nucleolar Organizer Regions (NORs) Polymorphism and Constitutive Heterochromatin of Moonlight Gourami, Trichopodus microlepis (Perciformes, Osphronemidae) |
| title_sort | first report on nucleolar organizer regions nors polymorphism and constitutive heterochromatin of moonlight gourami trichopodus microlepis perciformes osphronemidae |
| topic | Moonlight gourami, Trichopodus microlepis, Karyotype,,Nucleolar Organizer Region, Constitutive heterochromatin, Chromosome |
| url | https://riviste.fupress.net/index.php/caryologia/article/view/775 |
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