UPLC–MS Triglyceride Profiling in Sunflower and Rapeseed Seeds
Sunflower and rapeseed are among the most important sources of vegetable oil for food and industry. The main components of vegetable oil are triglycerides (TAGs) (about 97%). Ultra- performance liquid chromatography coupled with mass spectrometry (UPLC–MS) profiling of TAGs in sunflower an...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Biomolecules |
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| Online Access: | http://www.mdpi.com/2218-273X/9/1/9 |
| _version_ | 1819274338859220992 |
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| author | Alina Chernova Rim Gubaev Pavel Mazin Svetlana Goryunova Yakov Demurin Lyudmila Gorlova Anna Vanushkina Waltraud Mair Nikolai Anikanov Elena Martynova Denis Goryunov Sergei Garkusha Zhanna Mukhina Philipp Khaytovich |
| author_facet | Alina Chernova Rim Gubaev Pavel Mazin Svetlana Goryunova Yakov Demurin Lyudmila Gorlova Anna Vanushkina Waltraud Mair Nikolai Anikanov Elena Martynova Denis Goryunov Sergei Garkusha Zhanna Mukhina Philipp Khaytovich |
| author_sort | Alina Chernova |
| collection | DOAJ |
| description | Sunflower and rapeseed are among the most important sources of vegetable oil for food and industry. The main components of vegetable oil are triglycerides (TAGs) (about 97%). Ultra- performance liquid chromatography coupled with mass spectrometry (UPLC–MS) profiling of TAGs in sunflower and rapeseed has been performed and the TAG profiles obtained for these species have been compared. It has been identified that 34 TAGs are shared by sunflower and rapeseed. It was demonstrated that TAGs 52:2, 52:5, 52:6, 54:3; 54:4, 54:7, 56:3, 56:4, and 56:5 had the highest variability levels between sunflower and rapeseed with the higher presence in rapeseed. TAGs 50:2, 52:3, 52:4, 54:5, and 54:6 also showed high variability, but were the most abundant in sunflower. Moreover, the differences in TAG composition between the winter-type and spring-type rapeseed have been revealed, which may be associated with freezing tolerance. It was shown that winter-type rapeseed seeds contain TAGs with a lower degree of saturation, while in spring-type rapeseed highly saturated lipids are the most abundant. These findings may give new insights into the cold resistance mechanisms in plants the understanding of which is especially important in terms of global climate changes. |
| first_indexed | 2024-12-23T23:06:51Z |
| format | Article |
| id | doaj.art-f4d89ed5e0a9468b8e734da8d9a55d08 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-23T23:06:51Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-f4d89ed5e0a9468b8e734da8d9a55d082022-12-21T17:26:48ZengMDPI AGBiomolecules2218-273X2018-12-0191910.3390/biom9010009biom9010009UPLC–MS Triglyceride Profiling in Sunflower and Rapeseed SeedsAlina Chernova0Rim Gubaev1Pavel Mazin2Svetlana Goryunova3Yakov Demurin4Lyudmila Gorlova5Anna Vanushkina6Waltraud Mair7Nikolai Anikanov8Elena Martynova9Denis Goryunov10Sergei Garkusha11Zhanna Mukhina12Philipp Khaytovich13Center of Life Sciences, Skolkovo Institute of Science and Technology, Nobel st. 3, Building 1, Moscow 121205, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Nobel st. 3, Building 1, Moscow 121205, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Nobel st. 3, Building 1, Moscow 121205, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Nobel st. 3, Building 1, Moscow 121205, RussiaPustovoit All-Russia Research Institute of Oil Crops, Filatova st. 17, Krasnodar 350038, RussiaPustovoit All-Russia Research Institute of Oil Crops, Filatova st. 17, Krasnodar 350038, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Nobel st. 3, Building 1, Moscow 121205, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Nobel st. 3, Building 1, Moscow 121205, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Nobel st. 3, Building 1, Moscow 121205, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Nobel st. 3, Building 1, Moscow 121205, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Nobel st. 3, Building 1, Moscow 121205, RussiaAll-Russia Rice Research Institute, Belozerny 3, Krasnodar 350921, RussiaAll-Russia Rice Research Institute, Belozerny 3, Krasnodar 350921, RussiaCenter of Life Sciences, Skolkovo Institute of Science and Technology, Nobel st. 3, Building 1, Moscow 121205, RussiaSunflower and rapeseed are among the most important sources of vegetable oil for food and industry. The main components of vegetable oil are triglycerides (TAGs) (about 97%). Ultra- performance liquid chromatography coupled with mass spectrometry (UPLC–MS) profiling of TAGs in sunflower and rapeseed has been performed and the TAG profiles obtained for these species have been compared. It has been identified that 34 TAGs are shared by sunflower and rapeseed. It was demonstrated that TAGs 52:2, 52:5, 52:6, 54:3; 54:4, 54:7, 56:3, 56:4, and 56:5 had the highest variability levels between sunflower and rapeseed with the higher presence in rapeseed. TAGs 50:2, 52:3, 52:4, 54:5, and 54:6 also showed high variability, but were the most abundant in sunflower. Moreover, the differences in TAG composition between the winter-type and spring-type rapeseed have been revealed, which may be associated with freezing tolerance. It was shown that winter-type rapeseed seeds contain TAGs with a lower degree of saturation, while in spring-type rapeseed highly saturated lipids are the most abundant. These findings may give new insights into the cold resistance mechanisms in plants the understanding of which is especially important in terms of global climate changes.http://www.mdpi.com/2218-273X/9/1/9ultraperformance liquid chromatography–mass spectrometry (UPLC–MS)triglyceridessunflowerrapeseedwinter-type rapeseedspring-type rapeseedcold resistancefreezing tolerance |
| spellingShingle | Alina Chernova Rim Gubaev Pavel Mazin Svetlana Goryunova Yakov Demurin Lyudmila Gorlova Anna Vanushkina Waltraud Mair Nikolai Anikanov Elena Martynova Denis Goryunov Sergei Garkusha Zhanna Mukhina Philipp Khaytovich UPLC–MS Triglyceride Profiling in Sunflower and Rapeseed Seeds Biomolecules ultraperformance liquid chromatography–mass spectrometry (UPLC–MS) triglycerides sunflower rapeseed winter-type rapeseed spring-type rapeseed cold resistance freezing tolerance |
| title | UPLC–MS Triglyceride Profiling in Sunflower and Rapeseed Seeds |
| title_full | UPLC–MS Triglyceride Profiling in Sunflower and Rapeseed Seeds |
| title_fullStr | UPLC–MS Triglyceride Profiling in Sunflower and Rapeseed Seeds |
| title_full_unstemmed | UPLC–MS Triglyceride Profiling in Sunflower and Rapeseed Seeds |
| title_short | UPLC–MS Triglyceride Profiling in Sunflower and Rapeseed Seeds |
| title_sort | uplc ms triglyceride profiling in sunflower and rapeseed seeds |
| topic | ultraperformance liquid chromatography–mass spectrometry (UPLC–MS) triglycerides sunflower rapeseed winter-type rapeseed spring-type rapeseed cold resistance freezing tolerance |
| url | http://www.mdpi.com/2218-273X/9/1/9 |
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