Synchrotron Time-Lapse Imaging of Lignocellulosic Biomass Hydrolysis: Tracking Enzyme Localization by Protein Autofluorescence and Biochemical Modification of Cell Walls by Microfluidic Infrared Microspectroscopy

Synchrotron Time-Lapse Imaging of Lignocellulosic Biomass Hydrolysis: Tracking Enzyme Localization by Protein Autofluorescence and Biochemical Modification of Cell Walls by Microfluidic Infrared Microspectroscopy

Tracking enzyme localization and following the local biochemical modification of the substrate should help explain the recalcitrance of lignocellulosic plant cell walls to enzymatic degradation. Time-lapse studies using conventional imaging require enzyme labeling and following the biochemical modif...

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Bibliographic Details
Main Authors: Marie-Françoise Devaux, Frédéric Jamme, William André, Brigitte Bouchet, Camille Alvarado, Sylvie Durand, Paul Robert, Luc Saulnier, Estelle Bonnin, Fabienne Guillon
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-02-01
Series:Frontiers in Plant Science
Subjects:
spectral imaging
maize stem cells
cellulose and hemicellulose degradation
lignin
image analysis
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2018.00200/full

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