The modulation of platelet function by growth hormone in growth hormone deficient Hypopituitary patients
Abstract Background Growth hormone deficiency (GHD) has been implicated in increased cardiovascular and cerebrovascular disease risk seen in hypopituitarism, however the mechanism remains speculative. We hypothesise that platelet abnormalities may play a contributory role. Herein we examined platele...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Endocrine Disorders |
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| Online Access: | https://doi.org/10.1186/s12902-023-01448-6 |
| _version_ | 1797559147262115840 |
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| author | Irene K Oglesby David Slattery Nigel Glynn Saket Gupta Karen Duggan Martin Cuesta Eimear Dunne Aoife Garrahy Siobhan Toner Dermot Kenny Amar Agha |
| author_facet | Irene K Oglesby David Slattery Nigel Glynn Saket Gupta Karen Duggan Martin Cuesta Eimear Dunne Aoife Garrahy Siobhan Toner Dermot Kenny Amar Agha |
| author_sort | Irene K Oglesby |
| collection | DOAJ |
| description | Abstract Background Growth hormone deficiency (GHD) has been implicated in increased cardiovascular and cerebrovascular disease risk seen in hypopituitarism, however the mechanism remains speculative. We hypothesise that platelet abnormalities may play a contributory role. Herein we examined platelet behaviour in GHD hypopituitary patients, pre- and post-growth hormone (GH) replacement. Methods This study utilizes a physiological flow-based assay to quantify platelet function in whole blood from patient cohorts under arterial shear. Thirteen GH Naïve hypopituitary adults with GHD and thirteen healthy matched controls were studied. Patients were assessed before and after GH treatment. All other pituitary replacements were optimised before the study. In addition to a full endocrine profile, whole blood was labelled and perfused over immobilised von Willibrand factor (vWF). Seven parameters of dynamic platelet-vWF interactions were recorded using digital image microscopy and analysed by customised platelet tracking software. Results We found a significantly altered profile of platelet-vWF interactions in GHD individuals compared to healthy controls. Specifically, we observed a marked increase in platelets shown to form associations such as tethering, rolling and adherence to immobilized vWF, which were reduced post GH treatment. Speed and distance platelets travelled across vWF was similar between controls and pre-therapy GHD patients, however, this was considerably increased post treatment. This may indicate reduced platelet signaling resulting in less stable adhesion of platelets post GH treatment. Conclusions Taken together observed differences in platelet behaviour may contribute to an increased risk of thrombosis in GHD which can in part be reversed by GH therapy. |
| first_indexed | 2024-03-10T17:41:19Z |
| format | Article |
| id | doaj.art-f4d98874af7a4416926c43aec7e5118f |
| institution | Directory Open Access Journal |
| issn | 1472-6823 |
| language | English |
| last_indexed | 2024-03-10T17:41:19Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Endocrine Disorders |
| spelling | doaj.art-f4d98874af7a4416926c43aec7e5118f2023-11-20T09:42:19ZengBMCBMC Endocrine Disorders1472-68232023-09-0123111010.1186/s12902-023-01448-6The modulation of platelet function by growth hormone in growth hormone deficient Hypopituitary patientsIrene K Oglesby0David Slattery1Nigel Glynn2Saket Gupta3Karen Duggan4Martin Cuesta5Eimear Dunne6Aoife Garrahy7Siobhan Toner8Dermot Kenny9Amar Agha10Irish Centre for Vascular Biology and Molecular and Cellular Therapeutics, Royal College of Surgeons in IrelandDepartment of Endocrinology, Beaumont HospitalDepartment of Endocrinology, Beaumont HospitalDepartment of Endocrinology, Beaumont HospitalDepartment of Endocrinology, Beaumont HospitalDepartment of Endocrinology, Beaumont HospitalIrish Centre for Vascular Biology and Molecular and Cellular Therapeutics, Royal College of Surgeons in IrelandDepartment of Endocrinology, Beaumont HospitalDepartment of Endocrinology, Beaumont HospitalIrish Centre for Vascular Biology and Molecular and Cellular Therapeutics, Royal College of Surgeons in IrelandDepartment of Endocrinology, Beaumont HospitalAbstract Background Growth hormone deficiency (GHD) has been implicated in increased cardiovascular and cerebrovascular disease risk seen in hypopituitarism, however the mechanism remains speculative. We hypothesise that platelet abnormalities may play a contributory role. Herein we examined platelet behaviour in GHD hypopituitary patients, pre- and post-growth hormone (GH) replacement. Methods This study utilizes a physiological flow-based assay to quantify platelet function in whole blood from patient cohorts under arterial shear. Thirteen GH Naïve hypopituitary adults with GHD and thirteen healthy matched controls were studied. Patients were assessed before and after GH treatment. All other pituitary replacements were optimised before the study. In addition to a full endocrine profile, whole blood was labelled and perfused over immobilised von Willibrand factor (vWF). Seven parameters of dynamic platelet-vWF interactions were recorded using digital image microscopy and analysed by customised platelet tracking software. Results We found a significantly altered profile of platelet-vWF interactions in GHD individuals compared to healthy controls. Specifically, we observed a marked increase in platelets shown to form associations such as tethering, rolling and adherence to immobilized vWF, which were reduced post GH treatment. Speed and distance platelets travelled across vWF was similar between controls and pre-therapy GHD patients, however, this was considerably increased post treatment. This may indicate reduced platelet signaling resulting in less stable adhesion of platelets post GH treatment. Conclusions Taken together observed differences in platelet behaviour may contribute to an increased risk of thrombosis in GHD which can in part be reversed by GH therapy.https://doi.org/10.1186/s12902-023-01448-6HypopituitarismGrowth hormonePlateletsvon Willebrand factorThrombosisPlatelet-vWF interaction |
| spellingShingle | Irene K Oglesby David Slattery Nigel Glynn Saket Gupta Karen Duggan Martin Cuesta Eimear Dunne Aoife Garrahy Siobhan Toner Dermot Kenny Amar Agha The modulation of platelet function by growth hormone in growth hormone deficient Hypopituitary patients BMC Endocrine Disorders Hypopituitarism Growth hormone Platelets von Willebrand factor Thrombosis Platelet-vWF interaction |
| title | The modulation of platelet function by growth hormone in growth hormone deficient Hypopituitary patients |
| title_full | The modulation of platelet function by growth hormone in growth hormone deficient Hypopituitary patients |
| title_fullStr | The modulation of platelet function by growth hormone in growth hormone deficient Hypopituitary patients |
| title_full_unstemmed | The modulation of platelet function by growth hormone in growth hormone deficient Hypopituitary patients |
| title_short | The modulation of platelet function by growth hormone in growth hormone deficient Hypopituitary patients |
| title_sort | modulation of platelet function by growth hormone in growth hormone deficient hypopituitary patients |
| topic | Hypopituitarism Growth hormone Platelets von Willebrand factor Thrombosis Platelet-vWF interaction |
| url | https://doi.org/10.1186/s12902-023-01448-6 |
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