Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors
Abstract An effective wound management strategy needs accurate assessment of wound status throughout the whole healing process. This can be achieved by examining molecular biomarkers including proteins, DNAs, and RNAs. However, existing methods for quantifying these biomarkers such as immunohistoche...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-06-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202104835 |
| _version_ | 1828726399277465600 |
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| author | Jangsun Hwang Youngmin Seo Daun Jeong Xiaoyu Ning Christian Wiraja Lixia Yang Chew Teng Tan Jinhyuck Lee Yesol Kim Ji Won Kim Dai Hyun Kim Jonghoon Choi Chin Yan Lim Kanyi Pu Woo Young Jang Chenjie Xu |
| author_facet | Jangsun Hwang Youngmin Seo Daun Jeong Xiaoyu Ning Christian Wiraja Lixia Yang Chew Teng Tan Jinhyuck Lee Yesol Kim Ji Won Kim Dai Hyun Kim Jonghoon Choi Chin Yan Lim Kanyi Pu Woo Young Jang Chenjie Xu |
| author_sort | Jangsun Hwang |
| collection | DOAJ |
| description | Abstract An effective wound management strategy needs accurate assessment of wound status throughout the whole healing process. This can be achieved by examining molecular biomarkers including proteins, DNAs, and RNAs. However, existing methods for quantifying these biomarkers such as immunohistochemistry and quantitative polymerase chain reaction are usually laborious, resource‐intensive, and disruptive. This article reports the development and utilization of mRNA nanosensors (i.e., NanoFlare) that are topically applied on cutaneous wounds to reveal the healing status through targeted and semi‐quantitative examination of the mRNA biomarkers in skin cells. In 2D and 3D in vitro models, the efficacy and efficiency of these nanosensors are demonstrated in revealing the dynamic changes of mRNA biomarkers for different stages of wound development. In mouse models, this platform permits the tracking and identification of wound healing stages and a normal and diabetic wound healing process by wound healing index in real time. |
| first_indexed | 2024-04-12T13:42:34Z |
| format | Article |
| id | doaj.art-f4da08ae19224d939e0b526a10ed0bcb |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-12T13:42:34Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-f4da08ae19224d939e0b526a10ed0bcb2022-12-22T03:30:49ZengWileyAdvanced Science2198-38442022-06-01918n/an/a10.1002/advs.202104835Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA NanosensorsJangsun Hwang0Youngmin Seo1Daun Jeong2Xiaoyu Ning3Christian Wiraja4Lixia Yang5Chew Teng Tan6Jinhyuck Lee7Yesol Kim8Ji Won Kim9Dai Hyun Kim10Jonghoon Choi11Chin Yan Lim12Kanyi Pu13Woo Young Jang14Chenjie Xu15School of Chemical and Biomedical Engineering Nanyang Technological University 62 Nanyang Drive Singapore 637457 SingaporeSchool of Electrical and Electronic Engineering Yonsei University 50 Yonsei‐ro, Seodaemun‐gu Seoul 03722 Republic of KoreaDepartment of Orthopedic Surgery College of Medicine Korea University 73 Korea‐ro, Seongbuk‐gu Seoul 02841 Republic of KoreaSchool of Chemical and Biomedical Engineering Nanyang Technological University 62 Nanyang Drive Singapore 637457 SingaporeSchool of Chemical and Biomedical Engineering Nanyang Technological University 62 Nanyang Drive Singapore 637457 SingaporeSchool of Chemical and Biomedical Engineering Nanyang Technological University 62 Nanyang Drive Singapore 637457 SingaporeA*STAR Skin Research Labs Agency for Science Technology and Research 8A Biomedical Grove Singapore 138648 SingaporeDepartment of Orthopedic Surgery College of Medicine Korea University 73 Korea‐ro, Seongbuk‐gu Seoul 02841 Republic of KoreaSchool of Integrative Engineering Chung‐Ang University 84, Heukseok‐ro, Dongjak‐gu Seoul 06974 Republic of KoreaSchool of Integrative Engineering Chung‐Ang University 84, Heukseok‐ro, Dongjak‐gu Seoul 06974 Republic of KoreaDepartment of Dermatology College of Medicine Korea University 73 Korea‐ro, Seongbuk‐gu Seoul 02841 Republic of KoreaSchool of Integrative Engineering Chung‐Ang University 84, Heukseok‐ro, Dongjak‐gu Seoul 06974 Republic of KoreaA*STAR Skin Research Labs Agency for Science Technology and Research 8A Biomedical Grove Singapore 138648 SingaporeSchool of Chemical and Biomedical Engineering Nanyang Technological University 62 Nanyang Drive Singapore 637457 SingaporeDepartment of Orthopedic Surgery College of Medicine Korea University 73 Korea‐ro, Seongbuk‐gu Seoul 02841 Republic of KoreaDepartment of Biomedical Engineering City University of Hong Kong 83 Tat Chee Avenue Kowloon Hong Kong SAR ChinaAbstract An effective wound management strategy needs accurate assessment of wound status throughout the whole healing process. This can be achieved by examining molecular biomarkers including proteins, DNAs, and RNAs. However, existing methods for quantifying these biomarkers such as immunohistochemistry and quantitative polymerase chain reaction are usually laborious, resource‐intensive, and disruptive. This article reports the development and utilization of mRNA nanosensors (i.e., NanoFlare) that are topically applied on cutaneous wounds to reveal the healing status through targeted and semi‐quantitative examination of the mRNA biomarkers in skin cells. In 2D and 3D in vitro models, the efficacy and efficiency of these nanosensors are demonstrated in revealing the dynamic changes of mRNA biomarkers for different stages of wound development. In mouse models, this platform permits the tracking and identification of wound healing stages and a normal and diabetic wound healing process by wound healing index in real time.https://doi.org/10.1002/advs.202104835diabetic woundmRNA nanosensorsNanoFlarespherical nucleic acidswound healing |
| spellingShingle | Jangsun Hwang Youngmin Seo Daun Jeong Xiaoyu Ning Christian Wiraja Lixia Yang Chew Teng Tan Jinhyuck Lee Yesol Kim Ji Won Kim Dai Hyun Kim Jonghoon Choi Chin Yan Lim Kanyi Pu Woo Young Jang Chenjie Xu Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors Advanced Science diabetic wound mRNA nanosensors NanoFlare spherical nucleic acids wound healing |
| title | Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors |
| title_full | Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors |
| title_fullStr | Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors |
| title_full_unstemmed | Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors |
| title_short | Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors |
| title_sort | monitoring wound healing with topically applied optical nanoflare mrna nanosensors |
| topic | diabetic wound mRNA nanosensors NanoFlare spherical nucleic acids wound healing |
| url | https://doi.org/10.1002/advs.202104835 |
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