Targeted Delivery of Chimeric Antigen Receptor into T Cells via CRISPR-Mediated Homology-Directed Repair with a Dual-AAV6 Transduction System
CAR-T cell therapy involves genetically engineering T cells to recognize and attack tumour cells by adding a chimeric antigen receptor (CAR) to their surface. In this study, we have used dual transduction with AAV serotype 6 (AAV6) to integrate an anti-CD19 CAR into human T cells at a known genomic...
Main Authors: | Pablo D. Moço, Omar Farnós, David Sharon, Amine A. Kamen |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2023-09-01
|
Series: | Current Issues in Molecular Biology |
Subjects: | |
Online Access: | https://www.mdpi.com/1467-3045/45/10/486 |
Similar Items
-
Advancing chimeric antigen receptor T cell therapy with CRISPR/Cas9
by: Jiangtao Ren, et al.
Published: (2017-04-01) -
Population-wide gene disruption in the murine lung epithelium via AAV-mediated delivery of CRISPR-Cas9 components
by: Honglin Chen, et al.
Published: (2022-12-01) -
Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair
by: Natasa Savic, et al.
Published: (2018-05-01) -
AAV-CRISPR-Cas13 eliminates human enterovirus and prevents death of infected miceResearch in context
by: Choong Tat Keng, et al.
Published: (2023-07-01) -
On the Origins of Homology Directed Repair in Mammalian Cells
by: Brett M. Sansbury, et al.
Published: (2021-03-01)