High dilution rate of bull semen affects cryopreservation outcomes: kinematic and flowcytometric parameters
BACKGROUND: Semen cryopreservation might be influenced by sperm concentration. OBJECTIVES: This study was conducted to investigate three different semen concentrations [100 (C100), 50 (C50), and 25 (C25) × 106 spermatozoa/mL] on freezability of bull semen. METHODS: On each collection day, four ejac...
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| Format: | Article |
| Language: | English |
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University of Tehran
2017-10-01
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| Series: | Iranian Journal of Veterinary Medicine |
| Subjects: | |
| Online Access: | https://ijvm.ut.ac.ir/article_63763_a718b4bb27cb61ab0f9da9c5cec7d3f4.pdf |
| _version_ | 1818952220543025152 |
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| author | Sayed Mohammad Hadi Hussaini Mahdi Zhandi احمد زارع شحنه Mohsen Sharafi |
| author_facet | Sayed Mohammad Hadi Hussaini Mahdi Zhandi احمد زارع شحنه Mohsen Sharafi |
| author_sort | Sayed Mohammad Hadi Hussaini |
| collection | DOAJ |
| description | BACKGROUND: Semen cryopreservation might be influenced by sperm concentration. OBJECTIVES: This study was conducted to investigate three different semen concentrations [100 (C100), 50 (C50), and 25 (C25) × 106 spermatozoa/mL] on freezability of bull semen. METHODS: On each collection day, four ejaculates were collected (a total of 24 ejaculates from four bulls), pooled and divided to three equal parts. Each part was diluted to reach to one of the above mentioned final semen concentration and then frozen. After thawing, sperm motility, apoptosis status and mitochondrial activity were assessed. RESULTS: The results showed that C100 resulted in significantly higher total sperm motility compared to C50 and C25 groups. The percentage of live spermatozoa was significantly higher in C100 compared to C50 and C25 groups. Also, C25 resulted in significantly higher early and late apoptotic spermatozoa compared to C50 and C100 groups. The mitochondrial activity was significantly lower in C25 compared to C100 and C50 groups. CONCLUSIONS: It seems that low sperm concentrations (as low as 50×106) may be less advantageous for cryopreservation. |
| first_indexed | 2024-12-20T09:46:55Z |
| format | Article |
| id | doaj.art-f4ddeff75b6940b6aa8c9c6ef5feb06f |
| institution | Directory Open Access Journal |
| issn | 2251-8894 2252-0554 |
| language | English |
| last_indexed | 2024-12-20T09:46:55Z |
| publishDate | 2017-10-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Iranian Journal of Veterinary Medicine |
| spelling | doaj.art-f4ddeff75b6940b6aa8c9c6ef5feb06f2022-12-21T19:44:43ZengUniversity of TehranIranian Journal of Veterinary Medicine2251-88942252-05542017-10-0111437738310.22059/ijvm.2017.225373.100479063763High dilution rate of bull semen affects cryopreservation outcomes: kinematic and flowcytometric parametersSayed Mohammad Hadi Hussaini0Mahdi Zhandi1احمد زارع شحنه2Mohsen Sharafi3Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, IranDepartment of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, IranDepartment of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, IranDepartment of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran Department of Embryology at Reproduction Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACER, Tehran, IranBACKGROUND: Semen cryopreservation might be influenced by sperm concentration. OBJECTIVES: This study was conducted to investigate three different semen concentrations [100 (C100), 50 (C50), and 25 (C25) × 106 spermatozoa/mL] on freezability of bull semen. METHODS: On each collection day, four ejaculates were collected (a total of 24 ejaculates from four bulls), pooled and divided to three equal parts. Each part was diluted to reach to one of the above mentioned final semen concentration and then frozen. After thawing, sperm motility, apoptosis status and mitochondrial activity were assessed. RESULTS: The results showed that C100 resulted in significantly higher total sperm motility compared to C50 and C25 groups. The percentage of live spermatozoa was significantly higher in C100 compared to C50 and C25 groups. Also, C25 resulted in significantly higher early and late apoptotic spermatozoa compared to C50 and C100 groups. The mitochondrial activity was significantly lower in C25 compared to C100 and C50 groups. CONCLUSIONS: It seems that low sperm concentrations (as low as 50×106) may be less advantageous for cryopreservation.https://ijvm.ut.ac.ir/article_63763_a718b4bb27cb61ab0f9da9c5cec7d3f4.pdfbovineconcentrationflowcytometryfreezingsperm |
| spellingShingle | Sayed Mohammad Hadi Hussaini Mahdi Zhandi احمد زارع شحنه Mohsen Sharafi High dilution rate of bull semen affects cryopreservation outcomes: kinematic and flowcytometric parameters Iranian Journal of Veterinary Medicine bovine concentration flowcytometry freezing sperm |
| title | High dilution rate of bull semen affects cryopreservation outcomes: kinematic and flowcytometric parameters |
| title_full | High dilution rate of bull semen affects cryopreservation outcomes: kinematic and flowcytometric parameters |
| title_fullStr | High dilution rate of bull semen affects cryopreservation outcomes: kinematic and flowcytometric parameters |
| title_full_unstemmed | High dilution rate of bull semen affects cryopreservation outcomes: kinematic and flowcytometric parameters |
| title_short | High dilution rate of bull semen affects cryopreservation outcomes: kinematic and flowcytometric parameters |
| title_sort | high dilution rate of bull semen affects cryopreservation outcomes kinematic and flowcytometric parameters |
| topic | bovine concentration flowcytometry freezing sperm |
| url | https://ijvm.ut.ac.ir/article_63763_a718b4bb27cb61ab0f9da9c5cec7d3f4.pdf |
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