α‐Synuclein RT‐QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies
Abstract We applied RT‐QuIC assay to detect α‐synuclein aggregates in cerebrospinal fluid (CSF) of patients with suspected Creutzfeldt–Jakob disease who had a neuropathological diagnosis of dementia with Lewy bodies (DLB) (n = 7), other neurodegenerative diseases with α‐synuclein mixed pathology (n ...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-10-01
|
| Series: | Annals of Clinical and Translational Neurology |
| Online Access: | https://doi.org/10.1002/acn3.50897 |
| _version_ | 1828932962916237312 |
|---|---|
| author | Matilde Bongianni Anna Ladogana Stefano Capaldi Sigrid Klotz Simone Baiardi Annachiara Cagnin Daniela Perra Michele Fiorini Anna Poleggi Giuseppe Legname Tatiana Cattaruzza Francesco Janes Massimo Tabaton Bernardino Ghetti Salvatore Monaco Gabor G. Kovacs Piero Parchi Maurizio Pocchiari Gianluigi Zanusso |
| author_facet | Matilde Bongianni Anna Ladogana Stefano Capaldi Sigrid Klotz Simone Baiardi Annachiara Cagnin Daniela Perra Michele Fiorini Anna Poleggi Giuseppe Legname Tatiana Cattaruzza Francesco Janes Massimo Tabaton Bernardino Ghetti Salvatore Monaco Gabor G. Kovacs Piero Parchi Maurizio Pocchiari Gianluigi Zanusso |
| author_sort | Matilde Bongianni |
| collection | DOAJ |
| description | Abstract We applied RT‐QuIC assay to detect α‐synuclein aggregates in cerebrospinal fluid (CSF) of patients with suspected Creutzfeldt–Jakob disease who had a neuropathological diagnosis of dementia with Lewy bodies (DLB) (n = 7), other neurodegenerative diseases with α‐synuclein mixed pathology (n = 20), or without Lewy‐related pathology (n = 49). The test had a sensitivity of 92.9% and specificity of 95.9% in distinguishing α‐synucleinopathies from non‐α‐synucleinopathies. When performed in the CSF of patients with DLB (n = 36), RT‐QuIC was positive in 17/20 with probable DLB, 0/6 with possible DLB, and 0/10 with Alzheimer disease. These results indicate that RT‐QuIC for α‐synuclein is an accurate test for DLB diagnosis. |
| first_indexed | 2024-12-14T01:09:33Z |
| format | Article |
| id | doaj.art-f4deb6fe7ffd42969999bc511093e3f8 |
| institution | Directory Open Access Journal |
| issn | 2328-9503 |
| language | English |
| last_indexed | 2024-12-14T01:09:33Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Annals of Clinical and Translational Neurology |
| spelling | doaj.art-f4deb6fe7ffd42969999bc511093e3f82022-12-21T23:22:49ZengWileyAnnals of Clinical and Translational Neurology2328-95032019-10-016102120212610.1002/acn3.50897α‐Synuclein RT‐QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodiesMatilde Bongianni0Anna Ladogana1Stefano Capaldi2Sigrid Klotz3Simone Baiardi4Annachiara Cagnin5Daniela Perra6Michele Fiorini7Anna Poleggi8Giuseppe Legname9Tatiana Cattaruzza10Francesco Janes11Massimo Tabaton12Bernardino Ghetti13Salvatore Monaco14Gabor G. Kovacs15Piero Parchi16Maurizio Pocchiari17Gianluigi Zanusso18Department of Neurosciences, Biomedicine, and Movement Sciences University of Verona Policlinico G. B. Rossi Verona ItalyDepartment of Neuroscience Istituto Superiore di Sanità Rome ItalyBiocrystallography Laboratory Department of Biotechnology University of Verona Verona ItalyInstitute of Neurology Medical University of Vienna Vienna AustriaDepartment of Biomedical and Neuromotor Sciences University of Bologna Bologna ItalyDepartment of Neuroscience University of Padova Padova ItalyDepartment of Neurosciences, Biomedicine, and Movement Sciences University of Verona Policlinico G. B. Rossi Verona ItalyDepartment of Neurosciences, Biomedicine, and Movement Sciences University of Verona Policlinico G. B. Rossi Verona ItalyDepartment of Neuroscience Istituto Superiore di Sanità Rome ItalyLaboratory of Prion Biology Scuola Internazionale Superiore di Studi Avanzati (SISSA) Trieste ItalyNeurology Unit Department of Medicine, Surgery and Health Sciences University Hospital and Health Services of Trieste Trieste ItalyNeurology Unit University of Udine Academic Hospital Udine ItalyDepartment of Internal Medicine and Medical Specialties (DIMI) Unit of Geriatric Medicine University of Genova Genova ItalyDepartment of Pathology and Laboratory Medicine Indiana University School of Medicine Indianapolis IN USADepartment of Neurosciences, Biomedicine, and Movement Sciences University of Verona Policlinico G. B. Rossi Verona ItalyInstitute of Neurology Medical University of Vienna Vienna AustriaIRCCS Istituto delle Scienze Neurologiche di Bologna Bologna ItalyDepartment of Neuroscience Istituto Superiore di Sanità Rome ItalyDepartment of Neurosciences, Biomedicine, and Movement Sciences University of Verona Policlinico G. B. Rossi Verona ItalyAbstract We applied RT‐QuIC assay to detect α‐synuclein aggregates in cerebrospinal fluid (CSF) of patients with suspected Creutzfeldt–Jakob disease who had a neuropathological diagnosis of dementia with Lewy bodies (DLB) (n = 7), other neurodegenerative diseases with α‐synuclein mixed pathology (n = 20), or without Lewy‐related pathology (n = 49). The test had a sensitivity of 92.9% and specificity of 95.9% in distinguishing α‐synucleinopathies from non‐α‐synucleinopathies. When performed in the CSF of patients with DLB (n = 36), RT‐QuIC was positive in 17/20 with probable DLB, 0/6 with possible DLB, and 0/10 with Alzheimer disease. These results indicate that RT‐QuIC for α‐synuclein is an accurate test for DLB diagnosis.https://doi.org/10.1002/acn3.50897 |
| spellingShingle | Matilde Bongianni Anna Ladogana Stefano Capaldi Sigrid Klotz Simone Baiardi Annachiara Cagnin Daniela Perra Michele Fiorini Anna Poleggi Giuseppe Legname Tatiana Cattaruzza Francesco Janes Massimo Tabaton Bernardino Ghetti Salvatore Monaco Gabor G. Kovacs Piero Parchi Maurizio Pocchiari Gianluigi Zanusso α‐Synuclein RT‐QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies Annals of Clinical and Translational Neurology |
| title | α‐Synuclein RT‐QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies |
| title_full | α‐Synuclein RT‐QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies |
| title_fullStr | α‐Synuclein RT‐QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies |
| title_full_unstemmed | α‐Synuclein RT‐QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies |
| title_short | α‐Synuclein RT‐QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies |
| title_sort | α synuclein rt quic assay in cerebrospinal fluid of patients with dementia with lewy bodies |
| url | https://doi.org/10.1002/acn3.50897 |
| work_keys_str_mv | AT matildebongianni asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT annaladogana asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT stefanocapaldi asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT sigridklotz asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT simonebaiardi asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT annachiaracagnin asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT danielaperra asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT michelefiorini asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT annapoleggi asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT giuseppelegname asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT tatianacattaruzza asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT francescojanes asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT massimotabaton asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT bernardinoghetti asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT salvatoremonaco asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT gaborgkovacs asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT pieroparchi asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT mauriziopocchiari asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies AT gianluigizanusso asynucleinrtquicassayincerebrospinalfluidofpatientswithdementiawithlewybodies |