Comparison of the Quality of Protein Crystals Grown by CLPC Seeds Method

We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In additi...

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Bibliographic Details
Main Authors: Jin Li, Er-Kai Yan, Yue Liu, Zi-Qing Wu, Ya-Li Liu, Hai Hou, Chen-Yan Zhang, Qin-Qin Lu, Xu-Dong Deng, Da-Chuan Yin
Format: Article
Language:English
Published: MDPI AG 2019-09-01
Series:Crystals
Subjects:
Online Access:https://www.mdpi.com/2073-4352/9/10/501
Description
Summary:We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC seeds exhibited a better morphology. In addition, the X-ray diffraction data showed that the CLPC seeds method is a powerful tool to obtain high-quality protein crystals. Therefore, we recommend the use of CLPC seeds in preparing high-quality diffracting protein crystals.
ISSN:2073-4352