Efficacy of Triple Antibiotic Paste, Moxifloxacin, Calcium Hydroxide And 2% Chlorhexidine Gel In Elimination of E. Faecalis: An In vitro Study
Introduction: Root canal treatment is incomplete without usage of intra canal medicaments. They help in the reduction of bacterial count and its by-products, making canals clean and decreasing postoperative pains. Aim: The aim of this study was to evaluate and compare the antimicrobial activity...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/9132/22394_CE[Ra]_F(DK)_PF1(DK_NE)_PFA(DK)_PF2(SS_NE_DK).pdf |
| Summary: | Introduction: Root canal treatment is incomplete without
usage of intra canal medicaments. They help in the reduction
of bacterial count and its by-products, making canals clean and
decreasing postoperative pains.
Aim: The aim of this study was to evaluate and compare the
antimicrobial activity of triple antibiotic paste, Moxifloxacin,
calcium hydroxide and 2% Chlorhexidine (CHX) gel in elimination
of Enterococcous faecalis (E. faecalis).
Materials and Methods: Seventy-five root blocks were obtained
from extracted single rooted human teeth. The canal diameter
was increased using Gates- Glidden drill up to size 3 and then
contaminated with E. faecalis for 21 days. The contaminated
samples were then divided into following 5 groups. Group 1:
Saline (negative group), Group 2: Calcium hydroxide Ca(OH)2
,
Group 3: 2% CHX gel, Group 4: Triple Antibiotic Paste (TAP) (50
μg – metronidazole of 400 mg, 50 μg – minocycline of 100 mg,
50 μg – ciprofloxacin of 100 mg) and Group 5: Moxifloxacin (50
μg – moxifloxacin of 400 mg). Dentin debris was obtained at the
end of first, 7th, and 10th day using Gates Glidden drill sizes 4
and 5. The bacterial load was assessed by counting the number
of Colony Forming Units (CFUs). The data were analyzed with
the ANOVA and Post-Hoc tests to assess the differences in
antibacterial efficacy between groups (p=<0.001).
Results: A 2% CHX gel alone completely inhibited the growth of
E. faecalis after one, seven and 10 days. The 2% CHX gel was
the most effective medicament against E. faecalis, as it showed
significant differences with normal saline, calcium hydroxide,
Moxifloxacin or triple antibiotic paste at all time intervals. The
triple antibiotic paste group showed a moderate antibacterial
effect as its difference with all group was significantly better
at all days. Moxifloxacin was more effective than calcium
hydroxide on 7th and 10th day.
Conclusion: Best antimicrobial efficacy was shown by 2%
CHX gel. Moxifloxacin was equally efficient compared to triple
antibiotic paste against E. faecalis at longer intervals of time. |
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| ISSN: | 2249-782X 0973-709X |