Efficacy of Triple Antibiotic Paste, Moxifloxacin, Calcium Hydroxide And 2% Chlorhexidine Gel In Elimination of E. Faecalis: An In vitro Study
Introduction: Root canal treatment is incomplete without usage of intra canal medicaments. They help in the reduction of bacterial count and its by-products, making canals clean and decreasing postoperative pains. Aim: The aim of this study was to evaluate and compare the antimicrobial activity...
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JCDR Research and Publications Private Limited
2017-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/9132/22394_CE[Ra]_F(DK)_PF1(DK_NE)_PFA(DK)_PF2(SS_NE_DK).pdf |
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| author | Ashik Ali Lakhani K.S Sekhar Pankaj Gupta Bellam Tejolatha Anjali Gupta Shruti Kashyap Veena Desai Shanin Farista |
| author_facet | Ashik Ali Lakhani K.S Sekhar Pankaj Gupta Bellam Tejolatha Anjali Gupta Shruti Kashyap Veena Desai Shanin Farista |
| author_sort | Ashik Ali Lakhani |
| collection | DOAJ |
| description | Introduction: Root canal treatment is incomplete without
usage of intra canal medicaments. They help in the reduction
of bacterial count and its by-products, making canals clean and
decreasing postoperative pains.
Aim: The aim of this study was to evaluate and compare the
antimicrobial activity of triple antibiotic paste, Moxifloxacin,
calcium hydroxide and 2% Chlorhexidine (CHX) gel in elimination
of Enterococcous faecalis (E. faecalis).
Materials and Methods: Seventy-five root blocks were obtained
from extracted single rooted human teeth. The canal diameter
was increased using Gates- Glidden drill up to size 3 and then
contaminated with E. faecalis for 21 days. The contaminated
samples were then divided into following 5 groups. Group 1:
Saline (negative group), Group 2: Calcium hydroxide Ca(OH)2
,
Group 3: 2% CHX gel, Group 4: Triple Antibiotic Paste (TAP) (50
μg – metronidazole of 400 mg, 50 μg – minocycline of 100 mg,
50 μg – ciprofloxacin of 100 mg) and Group 5: Moxifloxacin (50
μg – moxifloxacin of 400 mg). Dentin debris was obtained at the
end of first, 7th, and 10th day using Gates Glidden drill sizes 4
and 5. The bacterial load was assessed by counting the number
of Colony Forming Units (CFUs). The data were analyzed with
the ANOVA and Post-Hoc tests to assess the differences in
antibacterial efficacy between groups (p=<0.001).
Results: A 2% CHX gel alone completely inhibited the growth of
E. faecalis after one, seven and 10 days. The 2% CHX gel was
the most effective medicament against E. faecalis, as it showed
significant differences with normal saline, calcium hydroxide,
Moxifloxacin or triple antibiotic paste at all time intervals. The
triple antibiotic paste group showed a moderate antibacterial
effect as its difference with all group was significantly better
at all days. Moxifloxacin was more effective than calcium
hydroxide on 7th and 10th day.
Conclusion: Best antimicrobial efficacy was shown by 2%
CHX gel. Moxifloxacin was equally efficient compared to triple
antibiotic paste against E. faecalis at longer intervals of time. |
| first_indexed | 2024-04-12T21:18:46Z |
| format | Article |
| id | doaj.art-f4e048a5d9d645f5b91db05e3c24fa7a |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-12T21:18:46Z |
| publishDate | 2017-01-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-f4e048a5d9d645f5b91db05e3c24fa7a2022-12-22T03:16:22ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-01-01111ZC06ZC0910.7860/JCDR/2017/22394.9132Efficacy of Triple Antibiotic Paste, Moxifloxacin, Calcium Hydroxide And 2% Chlorhexidine Gel In Elimination of E. Faecalis: An In vitro StudyAshik Ali Lakhani0K.S Sekhar1Pankaj Gupta2Bellam Tejolatha3Anjali Gupta4Shruti Kashyap5Veena Desai6Shanin Farista7Postgraduate Student, Department of Conservative Dentistry and Endodontics, Rungta College of Dental Science and Research Institute, Bhilai, Chhattisgarh, India.Head, Department of Conservative Dentistry and Endodontics, Rungta College of Dental Science and Research Institute, Bhilai, Chhattisgarh, India.Reader, Department of Conservative Dentistry and Endodontics, Rungta College of Dental Science and Research Institute, Bhilai, Chhattisgarh, India.Senior Lecture, Department of Conservative Dentistry and Endodontics, Rungta College of Dental Science and Research Institute, Bhilai, Chhattisgarh, India.Senior Lecture, Department of Conservative Dentistry and Endodontics, Rungta College of Dental Science and Research Institute, Bhilai, Chhattisgarh, India.Postgraduate Student, Department of Conservative Dentistry and Endodontics, Rungta College of Dental Science and Research Institute, Bhilai, Chhattisgarh, India.Postgraduate Student, Department of Oral and Maxillofacial Pathology, Rungta College of Dental Science and Research Institute, Bhilai, Chhattisgarh, India.Postgraduate Student, Department of Conservative Dentistry and Endodontics, Maitri Dentistry and Research Institute, Durg, Chhattisgarh, India.Introduction: Root canal treatment is incomplete without usage of intra canal medicaments. They help in the reduction of bacterial count and its by-products, making canals clean and decreasing postoperative pains. Aim: The aim of this study was to evaluate and compare the antimicrobial activity of triple antibiotic paste, Moxifloxacin, calcium hydroxide and 2% Chlorhexidine (CHX) gel in elimination of Enterococcous faecalis (E. faecalis). Materials and Methods: Seventy-five root blocks were obtained from extracted single rooted human teeth. The canal diameter was increased using Gates- Glidden drill up to size 3 and then contaminated with E. faecalis for 21 days. The contaminated samples were then divided into following 5 groups. Group 1: Saline (negative group), Group 2: Calcium hydroxide Ca(OH)2 , Group 3: 2% CHX gel, Group 4: Triple Antibiotic Paste (TAP) (50 μg – metronidazole of 400 mg, 50 μg – minocycline of 100 mg, 50 μg – ciprofloxacin of 100 mg) and Group 5: Moxifloxacin (50 μg – moxifloxacin of 400 mg). Dentin debris was obtained at the end of first, 7th, and 10th day using Gates Glidden drill sizes 4 and 5. The bacterial load was assessed by counting the number of Colony Forming Units (CFUs). The data were analyzed with the ANOVA and Post-Hoc tests to assess the differences in antibacterial efficacy between groups (p=<0.001). Results: A 2% CHX gel alone completely inhibited the growth of E. faecalis after one, seven and 10 days. The 2% CHX gel was the most effective medicament against E. faecalis, as it showed significant differences with normal saline, calcium hydroxide, Moxifloxacin or triple antibiotic paste at all time intervals. The triple antibiotic paste group showed a moderate antibacterial effect as its difference with all group was significantly better at all days. Moxifloxacin was more effective than calcium hydroxide on 7th and 10th day. Conclusion: Best antimicrobial efficacy was shown by 2% CHX gel. Moxifloxacin was equally efficient compared to triple antibiotic paste against E. faecalis at longer intervals of time.https://jcdr.net/articles/PDF/9132/22394_CE[Ra]_F(DK)_PF1(DK_NE)_PFA(DK)_PF2(SS_NE_DK).pdfantimicrobial efficacydentinal tubulefluoroquinoloneintra canal medicamentroot canal treatment |
| spellingShingle | Ashik Ali Lakhani K.S Sekhar Pankaj Gupta Bellam Tejolatha Anjali Gupta Shruti Kashyap Veena Desai Shanin Farista Efficacy of Triple Antibiotic Paste, Moxifloxacin, Calcium Hydroxide And 2% Chlorhexidine Gel In Elimination of E. Faecalis: An In vitro Study Journal of Clinical and Diagnostic Research antimicrobial efficacy dentinal tubule fluoroquinolone intra canal medicament root canal treatment |
| title | Efficacy of Triple Antibiotic Paste, Moxifloxacin, Calcium Hydroxide And 2% Chlorhexidine Gel In Elimination of E. Faecalis: An In vitro Study |
| title_full | Efficacy of Triple Antibiotic Paste, Moxifloxacin, Calcium Hydroxide And 2% Chlorhexidine Gel In Elimination of E. Faecalis: An In vitro Study |
| title_fullStr | Efficacy of Triple Antibiotic Paste, Moxifloxacin, Calcium Hydroxide And 2% Chlorhexidine Gel In Elimination of E. Faecalis: An In vitro Study |
| title_full_unstemmed | Efficacy of Triple Antibiotic Paste, Moxifloxacin, Calcium Hydroxide And 2% Chlorhexidine Gel In Elimination of E. Faecalis: An In vitro Study |
| title_short | Efficacy of Triple Antibiotic Paste, Moxifloxacin, Calcium Hydroxide And 2% Chlorhexidine Gel In Elimination of E. Faecalis: An In vitro Study |
| title_sort | efficacy of triple antibiotic paste moxifloxacin calcium hydroxide and 2 chlorhexidine gel in elimination of e faecalis an in vitro study |
| topic | antimicrobial efficacy dentinal tubule fluoroquinolone intra canal medicament root canal treatment |
| url | https://jcdr.net/articles/PDF/9132/22394_CE[Ra]_F(DK)_PF1(DK_NE)_PFA(DK)_PF2(SS_NE_DK).pdf |
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