The antimicrobial efficacy of nanographene oxide and double antibiotic paste per se and in combination: part II
Abstract Background Finding strategies to overcome the rising trends of antimicrobial resistance against currently available antimicrobial agents has become increasingly relevant. Graphene oxide has recently emerged as a promising material due to its outstanding physicochemical and biological proper...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-023-02957-5 |
| _version_ | 1797831921365942272 |
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| author | Fateme Eskandari Yasamin Ghahramani Abbas Abbaszadegan Ahmad Gholami |
| author_facet | Fateme Eskandari Yasamin Ghahramani Abbas Abbaszadegan Ahmad Gholami |
| author_sort | Fateme Eskandari |
| collection | DOAJ |
| description | Abstract Background Finding strategies to overcome the rising trends of antimicrobial resistance against currently available antimicrobial agents has become increasingly relevant. Graphene oxide has recently emerged as a promising material due to its outstanding physicochemical and biological properties. This study aimed to validate previous data on the antibacterial activity of nanographene oxide (nGO), double antibiotic paste (DAP), and their combination (nGO-DAP). Methods The antibacterial evaluation was performed against a wide range of microbial pathogens. Synthesis of nGO was achieved using a modified Hummers' method, and loading it with ciprofloxacin and metronidazole resulted in nGO-DAP. The microdilution method was utilized to assess the antimicrobial efficacy of nGO, DAP, and nGO-DAP against two gram-positive bacteria (S. aureus and E. faecalis), two gram-negative bacteria (E. coli, and S. typhi), and an opportunistic pathogenic yeast (C. albicans). Statistical analysis was conducted using one-sample t-test and one-way ANOVA (α = 0.05). Results All three antimicrobial agents significantly increased the killing percent of microbial pathogens compared to the control group (P < 0.05). Furthermore, the synthesized nGO-DAP exhibited higher antimicrobial activity than nGO and DAP per se. Conclusion The novel synthesized nGO-DAP can be used as an effective antimicrobial nanomaterial for use in dental, biomedical, and pharmaceutical fields against a range of microbial pathogens, including gram-negative and gram-positive bacteria, as well as yeasts. |
| first_indexed | 2024-04-09T13:59:29Z |
| format | Article |
| id | doaj.art-f4e38b6c2328435d89dbd6d4d8d9ad61 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-09T13:59:29Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-f4e38b6c2328435d89dbd6d4d8d9ad612023-05-07T11:25:59ZengBMCBMC Oral Health1472-68312023-05-0123111110.1186/s12903-023-02957-5The antimicrobial efficacy of nanographene oxide and double antibiotic paste per se and in combination: part IIFateme Eskandari0Yasamin Ghahramani1Abbas Abbaszadegan2Ahmad Gholami3Dentist, School of Dentistry, Shiraz University of Medical SciencesDepartment of Endodontics, School of Dentistry, Shiraz University of Medical SciencesDepartment of Endodontics, School of Dentistry, Shiraz University of Medical SciencesBiotechnology Research Center, Shiraz University of Medical SciencesAbstract Background Finding strategies to overcome the rising trends of antimicrobial resistance against currently available antimicrobial agents has become increasingly relevant. Graphene oxide has recently emerged as a promising material due to its outstanding physicochemical and biological properties. This study aimed to validate previous data on the antibacterial activity of nanographene oxide (nGO), double antibiotic paste (DAP), and their combination (nGO-DAP). Methods The antibacterial evaluation was performed against a wide range of microbial pathogens. Synthesis of nGO was achieved using a modified Hummers' method, and loading it with ciprofloxacin and metronidazole resulted in nGO-DAP. The microdilution method was utilized to assess the antimicrobial efficacy of nGO, DAP, and nGO-DAP against two gram-positive bacteria (S. aureus and E. faecalis), two gram-negative bacteria (E. coli, and S. typhi), and an opportunistic pathogenic yeast (C. albicans). Statistical analysis was conducted using one-sample t-test and one-way ANOVA (α = 0.05). Results All three antimicrobial agents significantly increased the killing percent of microbial pathogens compared to the control group (P < 0.05). Furthermore, the synthesized nGO-DAP exhibited higher antimicrobial activity than nGO and DAP per se. Conclusion The novel synthesized nGO-DAP can be used as an effective antimicrobial nanomaterial for use in dental, biomedical, and pharmaceutical fields against a range of microbial pathogens, including gram-negative and gram-positive bacteria, as well as yeasts.https://doi.org/10.1186/s12903-023-02957-5Antibacterial agentsAntimicrobialBiomedicalDentistryDouble antibiotic pasteEndodontics |
| spellingShingle | Fateme Eskandari Yasamin Ghahramani Abbas Abbaszadegan Ahmad Gholami The antimicrobial efficacy of nanographene oxide and double antibiotic paste per se and in combination: part II BMC Oral Health Antibacterial agents Antimicrobial Biomedical Dentistry Double antibiotic paste Endodontics |
| title | The antimicrobial efficacy of nanographene oxide and double antibiotic paste per se and in combination: part II |
| title_full | The antimicrobial efficacy of nanographene oxide and double antibiotic paste per se and in combination: part II |
| title_fullStr | The antimicrobial efficacy of nanographene oxide and double antibiotic paste per se and in combination: part II |
| title_full_unstemmed | The antimicrobial efficacy of nanographene oxide and double antibiotic paste per se and in combination: part II |
| title_short | The antimicrobial efficacy of nanographene oxide and double antibiotic paste per se and in combination: part II |
| title_sort | antimicrobial efficacy of nanographene oxide and double antibiotic paste per se and in combination part ii |
| topic | Antibacterial agents Antimicrobial Biomedical Dentistry Double antibiotic paste Endodontics |
| url | https://doi.org/10.1186/s12903-023-02957-5 |
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