Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats
Ovariectomy (OVX) causes a depletion of circulating estradiol (E2) and influences hypothalamic kisspeptin neurons, which govern gonadotropin-releasing hormone (GnRH) release and ultimately gonadotropin secretion. In this study, we examined the changes induced by OVX on the anterior pituitary gland i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2023-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2023/3143347 |
| _version_ | 1797867494196641792 |
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| author | Aki Oride Haruhiko Kanasaki Tuvshintugs Tumurbaatar Zolzaya Tumurgan Hiroe Okada Zhuoma Cairang Kyo Satoru |
| author_facet | Aki Oride Haruhiko Kanasaki Tuvshintugs Tumurbaatar Zolzaya Tumurgan Hiroe Okada Zhuoma Cairang Kyo Satoru |
| author_sort | Aki Oride |
| collection | DOAJ |
| description | Ovariectomy (OVX) causes a depletion of circulating estradiol (E2) and influences hypothalamic kisspeptin neurons, which govern gonadotropin-releasing hormone (GnRH) release and ultimately gonadotropin secretion. In this study, we examined the changes induced by OVX on the anterior pituitary gland in female rats. OVX significantly increased the mRNA expression of gonadotropin α, luteinizing hormone (LH) β, and follicle-stimulating hormone (FSH) β subunits within the pituitary gland compared with control (sham-operated) rats, and this was completely suppressed by E2 supplementation. High-dose dihydrotestosterone supplementation also prevented the OVX-induced increase in the expression of the three gonadotropin subunits. GnRH receptor mRNA expression within the pituitary was significantly increased in OVX rats, and this increase was completely inhibited by E2 supplementation. The mRNA expression of the receptors for adenylate cyclase-activating polypeptide and kisspeptin was unchanged by OVX. Although the mRNA levels of inhibin α, βA, and βB subunits within the pituitary gland were not modulated by OVX, follistatin gene expression within the pituitary gland was increased by OVX, and this increase was completely inhibited by E2 supplementation after OVX. In experiments using a pituitary gonadotroph cell model (LβT2 cells), follistatin itself did not modulate the mRNA expression of gonadotropin LHβ and FSHβ subunits, and the GnRH-induced increase in the expression of these genes was slightly inhibited in the presence of follistatin. Our current observations suggest that OVX induces several characteristic changes in the pituitary gland of rats. |
| first_indexed | 2024-04-09T23:41:05Z |
| format | Article |
| id | doaj.art-f4e5e590d22a462f855255f8897f61d9 |
| institution | Directory Open Access Journal |
| issn | 1687-8345 |
| language | English |
| last_indexed | 2024-04-09T23:41:05Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj.art-f4e5e590d22a462f855255f8897f61d92023-03-19T00:00:04ZengHindawi LimitedInternational Journal of Endocrinology1687-83452023-01-01202310.1155/2023/3143347Impact of Ovariectomy on the Anterior Pituitary Gland in Female RatsAki Oride0Haruhiko Kanasaki1Tuvshintugs Tumurbaatar2Zolzaya Tumurgan3Hiroe Okada4Zhuoma Cairang5Kyo Satoru6Department of Obstetrics and GynecologyDepartment of Obstetrics and GynecologyDepartment of Obstetrics and GynecologyDepartment of Obstetrics and GynecologyDepartment of Obstetrics and GynecologyDepartment of Obstetrics and GynecologyDepartment of Obstetrics and GynecologyOvariectomy (OVX) causes a depletion of circulating estradiol (E2) and influences hypothalamic kisspeptin neurons, which govern gonadotropin-releasing hormone (GnRH) release and ultimately gonadotropin secretion. In this study, we examined the changes induced by OVX on the anterior pituitary gland in female rats. OVX significantly increased the mRNA expression of gonadotropin α, luteinizing hormone (LH) β, and follicle-stimulating hormone (FSH) β subunits within the pituitary gland compared with control (sham-operated) rats, and this was completely suppressed by E2 supplementation. High-dose dihydrotestosterone supplementation also prevented the OVX-induced increase in the expression of the three gonadotropin subunits. GnRH receptor mRNA expression within the pituitary was significantly increased in OVX rats, and this increase was completely inhibited by E2 supplementation. The mRNA expression of the receptors for adenylate cyclase-activating polypeptide and kisspeptin was unchanged by OVX. Although the mRNA levels of inhibin α, βA, and βB subunits within the pituitary gland were not modulated by OVX, follistatin gene expression within the pituitary gland was increased by OVX, and this increase was completely inhibited by E2 supplementation after OVX. In experiments using a pituitary gonadotroph cell model (LβT2 cells), follistatin itself did not modulate the mRNA expression of gonadotropin LHβ and FSHβ subunits, and the GnRH-induced increase in the expression of these genes was slightly inhibited in the presence of follistatin. Our current observations suggest that OVX induces several characteristic changes in the pituitary gland of rats.http://dx.doi.org/10.1155/2023/3143347 |
| spellingShingle | Aki Oride Haruhiko Kanasaki Tuvshintugs Tumurbaatar Zolzaya Tumurgan Hiroe Okada Zhuoma Cairang Kyo Satoru Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats International Journal of Endocrinology |
| title | Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats |
| title_full | Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats |
| title_fullStr | Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats |
| title_full_unstemmed | Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats |
| title_short | Impact of Ovariectomy on the Anterior Pituitary Gland in Female Rats |
| title_sort | impact of ovariectomy on the anterior pituitary gland in female rats |
| url | http://dx.doi.org/10.1155/2023/3143347 |
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