Anaplasma phagocytophilum in ticks from parks in the Emilia-Romagna region of northern Italy
The aim of this study was to assess the prevalence and phylogenetic characterisation of Anaplasma phagocytophilum in ticks in three parks of the Emilia-Romagna region of northern Italy, an area in which no survey of this agent had previously been conducted. A total of 360 tick samples were analysed;...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2012-12-01
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| Series: | Veterinaria Italiana |
| Subjects: | |
| Online Access: | http://www.izs.it/vet_italiana/2012/48_4/413.pdf |
| _version_ | 1819103376224288768 |
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| author | Sara Aureli Janet E. Foley Roberta Galuppi Daniel Rejmanek Cristina Bonoli Maria Paola Tampieri |
| author_facet | Sara Aureli Janet E. Foley Roberta Galuppi Daniel Rejmanek Cristina Bonoli Maria Paola Tampieri |
| author_sort | Sara Aureli |
| collection | DOAJ |
| description | The aim of this study was to assess the prevalence and phylogenetic characterisation of Anaplasma phagocytophilum in ticks in three parks of the Emilia-Romagna region of northern Italy, an area in which no survey of this agent had previously been conducted. A total of 360 tick samples were analysed; 292 were sourced from the environment and 68 from animals and humans. Real-time polymerase chain reaction revealed that 33 tick samples (9.2%) were positive for A. phagocytophilum. Ixodes ricinus was the only species found positive from the samples retrieved from the environment and was the most commonly infected among ticks removed from hosts. Sequence analysis of the 23S-5S rRNA gene performed on 23 samples revealed six variant sequences that differed by only a few nucleotides when compared to the GenBank sequences from humans, horse and small mammals. Msp4 gene sequences obtained from 7 samples were compared to those described in ruminants, especially roe deer (Capreolus capreolus) and goat isolates from different countries. The results of this study provided evidence of the circulation of A. phagocytophilum in the sites studied and indicated the possible involvement of wild ruminants. Additional studies that extend the sampling areas, or cover different sites, would contribute to a better understanding of the ecology and disease dynamics of A. phagocytophilum in northern Italy and would provide valuable information on zoonotic risks. |
| first_indexed | 2024-12-22T01:49:28Z |
| format | Article |
| id | doaj.art-f4e979715d3444c49753870518bddf69 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-12-22T01:49:28Z |
| publishDate | 2012-12-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-f4e979715d3444c49753870518bddf692022-12-21T18:42:58ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272012-12-01484413423Anaplasma phagocytophilum in ticks from parks in the Emilia-Romagna region of northern ItalySara AureliJanet E. FoleyRoberta GaluppiDaniel RejmanekCristina BonoliMaria Paola TampieriThe aim of this study was to assess the prevalence and phylogenetic characterisation of Anaplasma phagocytophilum in ticks in three parks of the Emilia-Romagna region of northern Italy, an area in which no survey of this agent had previously been conducted. A total of 360 tick samples were analysed; 292 were sourced from the environment and 68 from animals and humans. Real-time polymerase chain reaction revealed that 33 tick samples (9.2%) were positive for A. phagocytophilum. Ixodes ricinus was the only species found positive from the samples retrieved from the environment and was the most commonly infected among ticks removed from hosts. Sequence analysis of the 23S-5S rRNA gene performed on 23 samples revealed six variant sequences that differed by only a few nucleotides when compared to the GenBank sequences from humans, horse and small mammals. Msp4 gene sequences obtained from 7 samples were compared to those described in ruminants, especially roe deer (Capreolus capreolus) and goat isolates from different countries. The results of this study provided evidence of the circulation of A. phagocytophilum in the sites studied and indicated the possible involvement of wild ruminants. Additional studies that extend the sampling areas, or cover different sites, would contribute to a better understanding of the ecology and disease dynamics of A. phagocytophilum in northern Italy and would provide valuable information on zoonotic risks.http://www.izs.it/vet_italiana/2012/48_4/413.pdfAnaplasma phagocytophilumAnaplasmosisEmilia-RomagnaItalyIxodes ricinusMsp4Polymerase chain reactionrRNATick. |
| spellingShingle | Sara Aureli Janet E. Foley Roberta Galuppi Daniel Rejmanek Cristina Bonoli Maria Paola Tampieri Anaplasma phagocytophilum in ticks from parks in the Emilia-Romagna region of northern Italy Veterinaria Italiana Anaplasma phagocytophilum Anaplasmosis Emilia-Romagna Italy Ixodes ricinus Msp4 Polymerase chain reaction rRNA Tick. |
| title | Anaplasma phagocytophilum in ticks from parks in the Emilia-Romagna region of northern Italy |
| title_full | Anaplasma phagocytophilum in ticks from parks in the Emilia-Romagna region of northern Italy |
| title_fullStr | Anaplasma phagocytophilum in ticks from parks in the Emilia-Romagna region of northern Italy |
| title_full_unstemmed | Anaplasma phagocytophilum in ticks from parks in the Emilia-Romagna region of northern Italy |
| title_short | Anaplasma phagocytophilum in ticks from parks in the Emilia-Romagna region of northern Italy |
| title_sort | anaplasma phagocytophilum in ticks from parks in the emilia romagna region of northern italy |
| topic | Anaplasma phagocytophilum Anaplasmosis Emilia-Romagna Italy Ixodes ricinus Msp4 Polymerase chain reaction rRNA Tick. |
| url | http://www.izs.it/vet_italiana/2012/48_4/413.pdf |
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