Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears
Abstract INTRODUCTION: Microscopic identification of active pulmonary tuberculosis (PTB) from direct smears of sputum (DS) is widely used for detection, but has limited sensitivity. Here, we assessed the yield of acid-fast bacilli (AFB) detection in processed sputum smears (PSS). METHODS: Sputum...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
|
| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000200237&lng=en&tlng=en |
| _version_ | 1828331764213350400 |
|---|---|
| author | José Luiz de Oliveira Magalhães Juliana Figueiredo da Costa Lima Ana Albertina de Araújo Ilyana Oliveira Coutinho Nilma Cintra Leal Alzira Maria Paiva de Almeida |
| author_facet | José Luiz de Oliveira Magalhães Juliana Figueiredo da Costa Lima Ana Albertina de Araújo Ilyana Oliveira Coutinho Nilma Cintra Leal Alzira Maria Paiva de Almeida |
| author_sort | José Luiz de Oliveira Magalhães |
| collection | DOAJ |
| description | Abstract INTRODUCTION: Microscopic identification of active pulmonary tuberculosis (PTB) from direct smears of sputum (DS) is widely used for detection, but has limited sensitivity. Here, we assessed the yield of acid-fast bacilli (AFB) detection in processed sputum smears (PSS). METHODS: Sputum samples were simultaneously analyzed by direct sputum smearing and after chemical treatment and spontaneous sedimentation. RESULTS: Of the 1,719 samples analyzed, 16.4% were positive for AFB in conventional DS and 21.4% in PSS, corresponding to a 30% increase in detection. CONCLUSIONS: Increased sensitivity from analyzing PSS and better safety protocols will contribute to improved detection and control of the disease. |
| first_indexed | 2024-04-13T20:56:47Z |
| format | Article |
| id | doaj.art-f4ea1a3f33d04635b8b61b2789b10cf4 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-04-13T20:56:47Z |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-f4ea1a3f33d04635b8b61b2789b10cf42022-12-22T02:30:17ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-984951223723910.1590/0037-8682-0238-2017S0037-86822018000200237Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smearsJosé Luiz de Oliveira MagalhãesJuliana Figueiredo da Costa LimaAna Albertina de AraújoIlyana Oliveira CoutinhoNilma Cintra LealAlzira Maria Paiva de AlmeidaAbstract INTRODUCTION: Microscopic identification of active pulmonary tuberculosis (PTB) from direct smears of sputum (DS) is widely used for detection, but has limited sensitivity. Here, we assessed the yield of acid-fast bacilli (AFB) detection in processed sputum smears (PSS). METHODS: Sputum samples were simultaneously analyzed by direct sputum smearing and after chemical treatment and spontaneous sedimentation. RESULTS: Of the 1,719 samples analyzed, 16.4% were positive for AFB in conventional DS and 21.4% in PSS, corresponding to a 30% increase in detection. CONCLUSIONS: Increased sensitivity from analyzing PSS and better safety protocols will contribute to improved detection and control of the disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000200237&lng=en&tlng=enTuberculosisSputum processedSmear microscopy |
| spellingShingle | José Luiz de Oliveira Magalhães Juliana Figueiredo da Costa Lima Ana Albertina de Araújo Ilyana Oliveira Coutinho Nilma Cintra Leal Alzira Maria Paiva de Almeida Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears Revista da Sociedade Brasileira de Medicina Tropical Tuberculosis Sputum processed Smear microscopy |
| title | Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears |
| title_full | Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears |
| title_fullStr | Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears |
| title_full_unstemmed | Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears |
| title_short | Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears |
| title_sort | microscopic detection of mycobacterium tuberculosis in direct or processed sputum smears |
| topic | Tuberculosis Sputum processed Smear microscopy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000200237&lng=en&tlng=en |
| work_keys_str_mv | AT joseluizdeoliveiramagalhaes microscopicdetectionofmycobacteriumtuberculosisindirectorprocessedsputumsmears AT julianafigueiredodacostalima microscopicdetectionofmycobacteriumtuberculosisindirectorprocessedsputumsmears AT anaalbertinadearaujo microscopicdetectionofmycobacteriumtuberculosisindirectorprocessedsputumsmears AT ilyanaoliveiracoutinho microscopicdetectionofmycobacteriumtuberculosisindirectorprocessedsputumsmears AT nilmacintraleal microscopicdetectionofmycobacteriumtuberculosisindirectorprocessedsputumsmears AT alziramariapaivadealmeida microscopicdetectionofmycobacteriumtuberculosisindirectorprocessedsputumsmears |