A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level
Abstract Background To determine if the rs7079 polymorphism located in the 3′ UTR of the angiotensinogen gene (AGT) altered AGT gene expression and the risk of lead poisoning. A case-control study and luciferase reporter gene assay identified a significant association between rs7079 variants and the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | Hereditas |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s41065-019-0084-x |
| _version_ | 1819225674480615424 |
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| author | Yu Wu Miaomiao Wang Jinlong Zhang Na Sun Chunping Li |
| author_facet | Yu Wu Miaomiao Wang Jinlong Zhang Na Sun Chunping Li |
| author_sort | Yu Wu |
| collection | DOAJ |
| description | Abstract Background To determine if the rs7079 polymorphism located in the 3′ UTR of the angiotensinogen gene (AGT) altered AGT gene expression and the risk of lead poisoning. A case-control study and luciferase reporter gene assay identified a significant association between rs7079 variants and the risk of lead poisoning. Results Serum AGT levels were significantly higher in individuals carrying the rs7079 CA genotype, as compared to those carrying the rs7079 CC genotype. The binding of the miRNA mimics miR-31-5p and miR-584-5p to the 3′ UTR of AGT differed based on which rs7079 variant was present, implying that AGT gene expression depends on the rs7079 variant carried. Conclusions The rs7079 C to A substitution reduced the binding of miR-31-5p/miR-584-5p to the 3′ UTR of AGT, possibly altering the risk of lead poisoning. |
| first_indexed | 2024-12-23T10:13:21Z |
| format | Article |
| id | doaj.art-f4eaafbd34d54c06ae4e9905ce258b74 |
| institution | Directory Open Access Journal |
| issn | 1601-5223 |
| language | English |
| last_indexed | 2024-12-23T10:13:21Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Hereditas |
| spelling | doaj.art-f4eaafbd34d54c06ae4e9905ce258b742022-12-21T17:50:53ZengBMCHereditas1601-52232019-01-0115611610.1186/s41065-019-0084-xA new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression levelYu Wu0Miaomiao Wang1Jinlong Zhang2Na Sun3Chunping Li4Wuxi Medical School, Jiangnan UniversityDepartment of Occupational Health, Wuxi Center for Disease Control and PreventionDepartment of Occupational Health, Wuxi Center for Disease Control and PreventionDepartment of Occupational Health, Wuxi Center for Disease Control and PreventionDepartment of Occupational Health, Wuxi Center for Disease Control and PreventionAbstract Background To determine if the rs7079 polymorphism located in the 3′ UTR of the angiotensinogen gene (AGT) altered AGT gene expression and the risk of lead poisoning. A case-control study and luciferase reporter gene assay identified a significant association between rs7079 variants and the risk of lead poisoning. Results Serum AGT levels were significantly higher in individuals carrying the rs7079 CA genotype, as compared to those carrying the rs7079 CC genotype. The binding of the miRNA mimics miR-31-5p and miR-584-5p to the 3′ UTR of AGT differed based on which rs7079 variant was present, implying that AGT gene expression depends on the rs7079 variant carried. Conclusions The rs7079 C to A substitution reduced the binding of miR-31-5p/miR-584-5p to the 3′ UTR of AGT, possibly altering the risk of lead poisoning.http://link.springer.com/article/10.1186/s41065-019-0084-xLead poisoning3′ untranslated region (3′ UTR)SNPmiRNAAGT |
| spellingShingle | Yu Wu Miaomiao Wang Jinlong Zhang Na Sun Chunping Li A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level Hereditas Lead poisoning 3′ untranslated region (3′ UTR) SNP miRNA AGT |
| title | A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level |
| title_full | A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level |
| title_fullStr | A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level |
| title_full_unstemmed | A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level |
| title_short | A new model of the mechanism underlying lead poisoning: SNP in miRNA target region influence the AGT expression level |
| title_sort | new model of the mechanism underlying lead poisoning snp in mirna target region influence the agt expression level |
| topic | Lead poisoning 3′ untranslated region (3′ UTR) SNP miRNA AGT |
| url | http://link.springer.com/article/10.1186/s41065-019-0084-x |
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