Bocaparvovirus infection in childrenin the republic of Belarus: molecular and epidemiological aspects
Objective: To study molecular and epidemiological aspects bocaparvovirus infection at the hospitalized, children in Republic of Belarus.Materials and methods: the studies were as part of a sentinel surveillance of influenza and. other agents of acute respiratory viral infections (ARVI) in the period...
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| Format: | Article |
| Language: | Russian |
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Journal Infectology
2019-12-01
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| Series: | Журнал инфектологии |
| Subjects: | |
| Online Access: | https://journal.niidi.ru/jofin/article/view/970 |
| _version_ | 1797723301335793664 |
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| author | N. V. Sivets N. P. Shmeleva T. P. Lapo |
| author_facet | N. V. Sivets N. P. Shmeleva T. P. Lapo |
| author_sort | N. V. Sivets |
| collection | DOAJ |
| description | Objective: To study molecular and epidemiological aspects bocaparvovirus infection at the hospitalized, children in Republic of Belarus.Materials and methods: the studies were as part of a sentinel surveillance of influenza and. other agents of acute respiratory viral infections (ARVI) in the period. 2010 — 2018. Investigated, nasopharyngeal swabs (3907), serum. (149) hospitalized. children from 0 to 18 years with symptoms of ARVI, as well as lymphoepithelial tissue of adenoids by real-time PCR (Rotor Gene 6000, Corbett research, Australia) for the presence of respiratory DNA/RNA viruses: influenza, respiratory syncytial virus, parainfluenza virus, rhinovirus, adenovirus, metapneumovirus, bocaparvovirus, and. coronavi-ruses. The full genomic DNA sequencing of the bocaparvovirus was performed, using the commercial kit «Genome Lab DTCS Quick Start Kit» (Beckman Coulter, USA). Electrophoresis and. analysis of the reaction products were performed, on a Beckman Coulter CEQ 8000 automated, capillary DNA analyzer (Beckman Coulter, USA).Results: the genetic material of respiratory viruses was detected, in 2781 cases (71,2%). Bocaparvovirus was detected in 337 (12,1%,) patients. Bocaparvovirus infection showed, an increase in the incidence rate in the autumn period. The most susceptible to this infection are children aged. 2 years to 4 years. The duration of viremia in these patients ranged, from 6 to 15 days. Phylogenetic analysis, the Belarusian, viruses HBoV1BLR/Mogilev/241/14, HBoV1BLR/Minsk/10/14, HBoV1BLR/Minsk/11/14, HBoV1BLR/Gomel/285/15 were combined into a separate group and showed genetic similarity with the ST2 virus. Analysis of the primary structure of the Belarusian bocaparvoviruses showed the presence of amino acid substitutions.Conclusion: Bocaparvovirus became the fourth, virus in frequency of occurrence along with other respiratory viruses. Bocaparvovirus infection may have a severe course. All amino acid, substitutions were located, in functionally significant regions of the virus genome. |
| first_indexed | 2024-03-12T10:00:55Z |
| format | Article |
| id | doaj.art-f4eb7db94dc840b78497ace450c7808a |
| institution | Directory Open Access Journal |
| issn | 2072-6732 |
| language | Russian |
| last_indexed | 2024-03-12T10:00:55Z |
| publishDate | 2019-12-01 |
| publisher | Journal Infectology |
| record_format | Article |
| series | Журнал инфектологии |
| spelling | doaj.art-f4eb7db94dc840b78497ace450c7808a2023-09-02T11:46:11ZrusJournal InfectologyЖурнал инфектологии2072-67322019-12-0111411312110.22625/2072-6732-2019-11-4-113-121770Bocaparvovirus infection in childrenin the republic of Belarus: molecular and epidemiological aspectsN. V. Sivets0N. P. Shmeleva1T. P. Lapo2Республиканский научно-практический центр эпидемиологии и микробиологииРеспубликанский научно-практический центр эпидемиологии и микробиологииРеспубликанский научно-практический центр эпидемиологии и микробиологииObjective: To study molecular and epidemiological aspects bocaparvovirus infection at the hospitalized, children in Republic of Belarus.Materials and methods: the studies were as part of a sentinel surveillance of influenza and. other agents of acute respiratory viral infections (ARVI) in the period. 2010 — 2018. Investigated, nasopharyngeal swabs (3907), serum. (149) hospitalized. children from 0 to 18 years with symptoms of ARVI, as well as lymphoepithelial tissue of adenoids by real-time PCR (Rotor Gene 6000, Corbett research, Australia) for the presence of respiratory DNA/RNA viruses: influenza, respiratory syncytial virus, parainfluenza virus, rhinovirus, adenovirus, metapneumovirus, bocaparvovirus, and. coronavi-ruses. The full genomic DNA sequencing of the bocaparvovirus was performed, using the commercial kit «Genome Lab DTCS Quick Start Kit» (Beckman Coulter, USA). Electrophoresis and. analysis of the reaction products were performed, on a Beckman Coulter CEQ 8000 automated, capillary DNA analyzer (Beckman Coulter, USA).Results: the genetic material of respiratory viruses was detected, in 2781 cases (71,2%). Bocaparvovirus was detected in 337 (12,1%,) patients. Bocaparvovirus infection showed, an increase in the incidence rate in the autumn period. The most susceptible to this infection are children aged. 2 years to 4 years. The duration of viremia in these patients ranged, from 6 to 15 days. Phylogenetic analysis, the Belarusian, viruses HBoV1BLR/Mogilev/241/14, HBoV1BLR/Minsk/10/14, HBoV1BLR/Minsk/11/14, HBoV1BLR/Gomel/285/15 were combined into a separate group and showed genetic similarity with the ST2 virus. Analysis of the primary structure of the Belarusian bocaparvoviruses showed the presence of amino acid substitutions.Conclusion: Bocaparvovirus became the fourth, virus in frequency of occurrence along with other respiratory viruses. Bocaparvovirus infection may have a severe course. All amino acid, substitutions were located, in functionally significant regions of the virus genome.https://journal.niidi.ru/jofin/article/view/970бокапарвовирус человекаполимеразная цепная реакциячастота выявлениявозрастная структурасезонностьсеквенированиефилогенетический анализ |
| spellingShingle | N. V. Sivets N. P. Shmeleva T. P. Lapo Bocaparvovirus infection in childrenin the republic of Belarus: molecular and epidemiological aspects Журнал инфектологии бокапарвовирус человека полимеразная цепная реакция частота выявления возрастная структура сезонность секвенирование филогенетический анализ |
| title | Bocaparvovirus infection in childrenin the republic of Belarus: molecular and epidemiological aspects |
| title_full | Bocaparvovirus infection in childrenin the republic of Belarus: molecular and epidemiological aspects |
| title_fullStr | Bocaparvovirus infection in childrenin the republic of Belarus: molecular and epidemiological aspects |
| title_full_unstemmed | Bocaparvovirus infection in childrenin the republic of Belarus: molecular and epidemiological aspects |
| title_short | Bocaparvovirus infection in childrenin the republic of Belarus: molecular and epidemiological aspects |
| title_sort | bocaparvovirus infection in childrenin the republic of belarus molecular and epidemiological aspects |
| topic | бокапарвовирус человека полимеразная цепная реакция частота выявления возрастная структура сезонность секвенирование филогенетический анализ |
| url | https://journal.niidi.ru/jofin/article/view/970 |
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