Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate
Owing to an increasing number of infections in adults, Lactococcus (L.) garvieae has gained recognition as an emerging human pathogen, causing bacteraemia and septicaemia. In September 2020, four paediatric onco-hematologic patients received a platelet concentrate from the same adult donor at Bambin...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2022.2071174 |
| _version_ | 1818231188789133312 |
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| author | Luna Colagrossi Valentino Costabile Rossana Scutari Marilena Agosta Manuela Onori Livia Mancinelli Barbara Lucignano Andrea Onetti Muda Giada Del Baldo Angela Mastronuzzi Franco Locatelli Guglielmo Trua Mauro Montanari Claudia Alteri Paola Bernaschi Carlo Federico Perno |
| author_facet | Luna Colagrossi Valentino Costabile Rossana Scutari Marilena Agosta Manuela Onori Livia Mancinelli Barbara Lucignano Andrea Onetti Muda Giada Del Baldo Angela Mastronuzzi Franco Locatelli Guglielmo Trua Mauro Montanari Claudia Alteri Paola Bernaschi Carlo Federico Perno |
| author_sort | Luna Colagrossi |
| collection | DOAJ |
| description | Owing to an increasing number of infections in adults, Lactococcus (L.) garvieae has gained recognition as an emerging human pathogen, causing bacteraemia and septicaemia. In September 2020, four paediatric onco-hematologic patients received a platelet concentrate from the same adult donor at Bambino Gesù Children’s Hospital IRCCS, Rome. Three of four patients experienced L. garvieae sepsis one day after transfusion. The L. garvieae pediatric isolates and the donor’s platelet concentrates were retrospectively collected for whole-genome sequencing and shot-gun metagenomics, respectively (Illumina HiSeq). By de novo assembly of the L. garvieae genomes, we found that all three pediatric isolates shared a 99.9% identity and were characterized by 440 common SNPs. Plasmid pUC11C (conferring virulence properties) and the temperate prophage Plg-Tb25 were detected in all three strains. Core SNP genome-based maximum likelihood and Bayesian trees confirmed their phylogenetic common origin and revealed their relationship with L. garvieae strains affecting cows and humans (bootstrap values >100 and posterior probabilities = 1.00). Bacterial reads obtained by the donor’s platelet concentrate have been profiled with MetaPhlAn2 (v.2.7.5); among these, 29.9% belonged to Firmicutes, and 5.16% to Streptococcaceae (>97% identity with L. garvieae), confirming the presence of L. garvieae in the platelet concentrate transfusion. These data showed three episodes of sepsis for the first time due to a transfusion-associated transmission of L. garvieae in three pediatric hospitalized hematology patients. This highlights the importance to implement the screening of platelet components with new human-defined pathogens for ensuring the safety of blood supply, and more broadly, for the surveillance of emerging pathogens. |
| first_indexed | 2024-12-12T10:46:26Z |
| format | Article |
| id | doaj.art-f4ed4ba67f2540c78a1a7ac1c4e4277f |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-12-12T10:46:26Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-f4ed4ba67f2540c78a1a7ac1c4e4277f2022-12-22T00:26:52ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512022-12-011111325133410.1080/22221751.2022.2071174Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrateLuna Colagrossi0Valentino Costabile1Rossana Scutari2Marilena Agosta3Manuela Onori4Livia Mancinelli5Barbara Lucignano6Andrea Onetti Muda7Giada Del Baldo8Angela Mastronuzzi9Franco Locatelli10Guglielmo Trua11Mauro Montanari12Claudia Alteri13Paola Bernaschi14Carlo Federico Perno15Department of Laboratories, Unit of Diagnostic Microbiology and Immunology and Multimodal Medicine Area, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Laboratories, Unit of Diagnostic Microbiology and Immunology and Multimodal Medicine Area, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Laboratories, Unit of Diagnostic Microbiology and Immunology and Multimodal Medicine Area, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Laboratories, Unit of Diagnostic Microbiology and Immunology and Multimodal Medicine Area, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Laboratories, Unit of Diagnostic Microbiology and Immunology and Multimodal Medicine Area, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Laboratories, Unit of Diagnostic Microbiology and Immunology and Multimodal Medicine Area, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Laboratories, Unit of Diagnostic Microbiology and Immunology and Multimodal Medicine Area, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Laboratories, Unit of Diagnostic Microbiology and Immunology and Multimodal Medicine Area, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Pediatric Hematology/Oncology and Cellular and Gene Therapy, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Pediatric Hematology/Oncology and Cellular and Gene Therapy, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Pediatric Hematology/Oncology and Cellular and Gene Therapy, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Transfusion Medicine, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Transfusion Medicine, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Laboratories, Unit of Diagnostic Microbiology and Immunology and Multimodal Medicine Area, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Laboratories, Unit of Diagnostic Microbiology and Immunology and Multimodal Medicine Area, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyDepartment of Laboratories, Unit of Diagnostic Microbiology and Immunology and Multimodal Medicine Area, Bambino Gesù Children’s Hospital IRCCS, Rome, ItalyOwing to an increasing number of infections in adults, Lactococcus (L.) garvieae has gained recognition as an emerging human pathogen, causing bacteraemia and septicaemia. In September 2020, four paediatric onco-hematologic patients received a platelet concentrate from the same adult donor at Bambino Gesù Children’s Hospital IRCCS, Rome. Three of four patients experienced L. garvieae sepsis one day after transfusion. The L. garvieae pediatric isolates and the donor’s platelet concentrates were retrospectively collected for whole-genome sequencing and shot-gun metagenomics, respectively (Illumina HiSeq). By de novo assembly of the L. garvieae genomes, we found that all three pediatric isolates shared a 99.9% identity and were characterized by 440 common SNPs. Plasmid pUC11C (conferring virulence properties) and the temperate prophage Plg-Tb25 were detected in all three strains. Core SNP genome-based maximum likelihood and Bayesian trees confirmed their phylogenetic common origin and revealed their relationship with L. garvieae strains affecting cows and humans (bootstrap values >100 and posterior probabilities = 1.00). Bacterial reads obtained by the donor’s platelet concentrate have been profiled with MetaPhlAn2 (v.2.7.5); among these, 29.9% belonged to Firmicutes, and 5.16% to Streptococcaceae (>97% identity with L. garvieae), confirming the presence of L. garvieae in the platelet concentrate transfusion. These data showed three episodes of sepsis for the first time due to a transfusion-associated transmission of L. garvieae in three pediatric hospitalized hematology patients. This highlights the importance to implement the screening of platelet components with new human-defined pathogens for ensuring the safety of blood supply, and more broadly, for the surveillance of emerging pathogens.https://www.tandfonline.com/doi/10.1080/22221751.2022.2071174Sepsispediatric sepsisonco-hematologyblood transfusionLactococcus garvieaepathogen transmission |
| spellingShingle | Luna Colagrossi Valentino Costabile Rossana Scutari Marilena Agosta Manuela Onori Livia Mancinelli Barbara Lucignano Andrea Onetti Muda Giada Del Baldo Angela Mastronuzzi Franco Locatelli Guglielmo Trua Mauro Montanari Claudia Alteri Paola Bernaschi Carlo Federico Perno Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate Emerging Microbes and Infections Sepsis pediatric sepsis onco-hematology blood transfusion Lactococcus garvieae pathogen transmission |
| title | Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate |
| title_full | Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate |
| title_fullStr | Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate |
| title_full_unstemmed | Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate |
| title_short | Evidence of pediatric sepsis caused by a drug resistant Lactococcus garvieae contaminated platelet concentrate |
| title_sort | evidence of pediatric sepsis caused by a drug resistant lactococcus garvieae contaminated platelet concentrate |
| topic | Sepsis pediatric sepsis onco-hematology blood transfusion Lactococcus garvieae pathogen transmission |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2022.2071174 |
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