Ovocystatin Induced Changes in Expression of Alzheimer’s Disease Relevant Proteins in APP/PS1 Transgenic Mice
Background: Ovocystatin is marked by structural and biological similarities to human cystatin C, which plays an important role in the course of neurodegenerative diseases. Recently, it has been shown that ovocystatin might prevent aging-related cognitive impairment in rats and reduce memory decline...
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MDPI AG
2022-04-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/9/2372 |
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| author | Bartlomiej Stanczykiewicz Jakub Gburek Maria Rutkowska Marta Lemieszewska Krzysztof Gołąb Katarzyna Juszczyńska Aleksandra Piotrowska Tadeusz Trziszka Piotr Dzięgiel Marzenna Podhorska-Okołów Agnieszka Zabłocka Joanna Rymaszewska |
| author_facet | Bartlomiej Stanczykiewicz Jakub Gburek Maria Rutkowska Marta Lemieszewska Krzysztof Gołąb Katarzyna Juszczyńska Aleksandra Piotrowska Tadeusz Trziszka Piotr Dzięgiel Marzenna Podhorska-Okołów Agnieszka Zabłocka Joanna Rymaszewska |
| author_sort | Bartlomiej Stanczykiewicz |
| collection | DOAJ |
| description | Background: Ovocystatin is marked by structural and biological similarities to human cystatin C, which plays an important role in the course of neurodegenerative diseases. Recently, it has been shown that ovocystatin might prevent aging-related cognitive impairment in rats and reduce memory decline in an APP/PS1 mice model. Thus, this study aimed to assess the effect of ovocystatin on histopathological changes in APP/PS1 mice. Materials and methods: Ovocystatin was administered intraperitoneally for four weeks (40 μg/mouse) to 35-weeks-old transgenic (AD, n = 14) and wild type (NCAR, n = 15) mice (stock B6C3-Tg(APPswe, PSEN1dE9)85Dbo/Mmjax). A histopathological evaluation comprised antibodies directed against β-amyloid (1:400, SIG-39320-1000, Covance) and Tau (1:4000, AHB0042, Invitrogen). Three regions of the hippocampus— the dentate gyrus (DG) and the cornu ammonis (CA1 and CA3)—were analyzed by immunohistochemistry in each animal. All differences are expressed as percentage relative to the control group. Results: The main results showed that the percentage of immunoreactive area of β-amyloid, tau protein deposits in APP/PS1+ovCYS was decreased in DG, CA1, and CA3 regions compared with the APP/PS1 control, respectively (<i>p</i> < 0.05). Conclusions: Ovocystatin caused significant changes in the expression pattern of all investigated proteins in hippocampal tissues both in APP/PS1 and NCAR mice. |
| first_indexed | 2024-03-10T04:02:56Z |
| format | Article |
| id | doaj.art-f4ee102726b647a496ceab5f38470f3e |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T04:02:56Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-f4ee102726b647a496ceab5f38470f3e2023-11-23T08:31:26ZengMDPI AGJournal of Clinical Medicine2077-03832022-04-01119237210.3390/jcm11092372Ovocystatin Induced Changes in Expression of Alzheimer’s Disease Relevant Proteins in APP/PS1 Transgenic MiceBartlomiej Stanczykiewicz0Jakub Gburek1Maria Rutkowska2Marta Lemieszewska3Krzysztof Gołąb4Katarzyna Juszczyńska5Aleksandra Piotrowska6Tadeusz Trziszka7Piotr Dzięgiel8Marzenna Podhorska-Okołów9Agnieszka Zabłocka10Joanna Rymaszewska11Department of Psychiatry, Division of Consultation Psychiatry and Neuroscience, Wroclaw Medical University, 50-367 Wroclaw, PolandDepartment of Pharmaceutical Biochemistry, Wroclaw Medical University, 50-556 Wroclaw, PolandDepartment of Pharmacology, Wroclaw Medical University, 50-345 Wroclaw, PolandDepartment of Psychiatry, Division of Consultation Psychiatry and Neuroscience, Wroclaw Medical University, 50-367 Wroclaw, PolandDepartment of Pharmaceutical Biochemistry, Wroclaw Medical University, 50-556 Wroclaw, PolandDepartment of Pharmaceutical Biochemistry, Wroclaw Medical University, 50-556 Wroclaw, PolandDepartment of Human Morphology and Embryology, Division of Histology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDepartment of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences, 51-630 Wroclaw, PolandDepartment of Human Morphology and Embryology, Division of Histology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Ultrastructure Research, Wroclaw Medical University, 50-368 Wroclaw, PolandDepartment of Microbiology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, PolandDepartment of Psychiatry, Division of Consultation Psychiatry and Neuroscience, Wroclaw Medical University, 50-367 Wroclaw, PolandBackground: Ovocystatin is marked by structural and biological similarities to human cystatin C, which plays an important role in the course of neurodegenerative diseases. Recently, it has been shown that ovocystatin might prevent aging-related cognitive impairment in rats and reduce memory decline in an APP/PS1 mice model. Thus, this study aimed to assess the effect of ovocystatin on histopathological changes in APP/PS1 mice. Materials and methods: Ovocystatin was administered intraperitoneally for four weeks (40 μg/mouse) to 35-weeks-old transgenic (AD, n = 14) and wild type (NCAR, n = 15) mice (stock B6C3-Tg(APPswe, PSEN1dE9)85Dbo/Mmjax). A histopathological evaluation comprised antibodies directed against β-amyloid (1:400, SIG-39320-1000, Covance) and Tau (1:4000, AHB0042, Invitrogen). Three regions of the hippocampus— the dentate gyrus (DG) and the cornu ammonis (CA1 and CA3)—were analyzed by immunohistochemistry in each animal. All differences are expressed as percentage relative to the control group. Results: The main results showed that the percentage of immunoreactive area of β-amyloid, tau protein deposits in APP/PS1+ovCYS was decreased in DG, CA1, and CA3 regions compared with the APP/PS1 control, respectively (<i>p</i> < 0.05). Conclusions: Ovocystatin caused significant changes in the expression pattern of all investigated proteins in hippocampal tissues both in APP/PS1 and NCAR mice.https://www.mdpi.com/2077-0383/11/9/2372ovocystatinchicken cystatincystatin CAlzheimer’s diseasemice |
| spellingShingle | Bartlomiej Stanczykiewicz Jakub Gburek Maria Rutkowska Marta Lemieszewska Krzysztof Gołąb Katarzyna Juszczyńska Aleksandra Piotrowska Tadeusz Trziszka Piotr Dzięgiel Marzenna Podhorska-Okołów Agnieszka Zabłocka Joanna Rymaszewska Ovocystatin Induced Changes in Expression of Alzheimer’s Disease Relevant Proteins in APP/PS1 Transgenic Mice Journal of Clinical Medicine ovocystatin chicken cystatin cystatin C Alzheimer’s disease mice |
| title | Ovocystatin Induced Changes in Expression of Alzheimer’s Disease Relevant Proteins in APP/PS1 Transgenic Mice |
| title_full | Ovocystatin Induced Changes in Expression of Alzheimer’s Disease Relevant Proteins in APP/PS1 Transgenic Mice |
| title_fullStr | Ovocystatin Induced Changes in Expression of Alzheimer’s Disease Relevant Proteins in APP/PS1 Transgenic Mice |
| title_full_unstemmed | Ovocystatin Induced Changes in Expression of Alzheimer’s Disease Relevant Proteins in APP/PS1 Transgenic Mice |
| title_short | Ovocystatin Induced Changes in Expression of Alzheimer’s Disease Relevant Proteins in APP/PS1 Transgenic Mice |
| title_sort | ovocystatin induced changes in expression of alzheimer s disease relevant proteins in app ps1 transgenic mice |
| topic | ovocystatin chicken cystatin cystatin C Alzheimer’s disease mice |
| url | https://www.mdpi.com/2077-0383/11/9/2372 |
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