Role of SIRT5 in the analgesic effectiveness of moxibustion at ST36 in mice with inflammatory pain
Sirtuine5 (SIRT5) is an important molecule involved in the pathology of inflammatory diseases. To investigate the impact of SIRT5 on the analgesic effectiveness of moxibustion, we established a complete Freund's adjuvant- (CFA-) induced inflammatory pain in mice model. Moxibustion was applied a...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023049733 |
| _version_ | 1797771534043971584 |
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| author | Chuan-yi Zuo Chun-yan Gou Cheng-shun Zhang Xi Zhou Peng Lv Han-xiao Zhang Zheng-peng Fan Feng-wei Tian Zhu-xing Wang |
| author_facet | Chuan-yi Zuo Chun-yan Gou Cheng-shun Zhang Xi Zhou Peng Lv Han-xiao Zhang Zheng-peng Fan Feng-wei Tian Zhu-xing Wang |
| author_sort | Chuan-yi Zuo |
| collection | DOAJ |
| description | Sirtuine5 (SIRT5) is an important molecule involved in the pathology of inflammatory diseases. To investigate the impact of SIRT5 on the analgesic effectiveness of moxibustion, we established a complete Freund's adjuvant- (CFA-) induced inflammatory pain in mice model. Moxibustion was applied at the Zusanli (ST36) acupoint in mice with inflammatory pain. The analgesic effectiveness was evaluated by thermal hyperalgesia and mechanical allodynia tests in the right paws after CFA injection. The expression of inflammatory cytokines, including the pro-inflammatory factors IL-1β and TNF-α, and the anti-inflammatory factors IL-4 and TGF-β expressions, was evaluated using by ELISA. Furthermore, SIRT5 was evaluated by immunofluorescence and western blotting. The results showed that, compared with the CFA group, both thermal and mechanical pain thresholds increased with moxibustion and the SIRT5 inhibitor MC3482 intervention at ST36. Additionally, compared to the CFA-induced group, the inflammatory mediators, including IL-1β and TNF-α, decreased, while the anti-inflammatory cytokines IL-4 and TGF-β increased with moxibustion and MC3482 ST36 acupoint injection. Western blot results showed a decreased expression of SIRT5 at the ST36 site with moxibustion and MC3482 injection, compared to the CFA-induced group. SIRT5 expression in the right paw of mice injected with moxibustion and MC3482 was higher than that in the CFA-induced group. This study revealed that SIRT5 expression is involved in moxibustion analgesia and may be a potential mediator in the regulation of analgesia. |
| first_indexed | 2024-03-12T21:38:59Z |
| format | Article |
| id | doaj.art-f4f06a2d58a04067912ee0d7247d35b8 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T21:38:59Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-f4f06a2d58a04067912ee0d7247d35b82023-07-27T05:57:22ZengElsevierHeliyon2405-84402023-07-0197e17765Role of SIRT5 in the analgesic effectiveness of moxibustion at ST36 in mice with inflammatory painChuan-yi Zuo0Chun-yan Gou1Cheng-shun Zhang2Xi Zhou3Peng Lv4Han-xiao Zhang5Zheng-peng Fan6Feng-wei Tian7Zhu-xing Wang8Department of Acupuncture, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, Chongqing, ChinaDepartment of Acupuncture, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, Chongqing, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan, ChinaDepartment of Acupuncture, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, Chongqing, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan, ChinaFaculty of Medicine, Université Paris-Saclay, Villejuif, 94800, FranceDepartment of Acupuncture, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, Chongqing, ChinaDepartment of Acupuncture, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, Chongqing, China; Corresponding author.Department of Acupuncture, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, Chongqing, China; Corresponding author.Sirtuine5 (SIRT5) is an important molecule involved in the pathology of inflammatory diseases. To investigate the impact of SIRT5 on the analgesic effectiveness of moxibustion, we established a complete Freund's adjuvant- (CFA-) induced inflammatory pain in mice model. Moxibustion was applied at the Zusanli (ST36) acupoint in mice with inflammatory pain. The analgesic effectiveness was evaluated by thermal hyperalgesia and mechanical allodynia tests in the right paws after CFA injection. The expression of inflammatory cytokines, including the pro-inflammatory factors IL-1β and TNF-α, and the anti-inflammatory factors IL-4 and TGF-β expressions, was evaluated using by ELISA. Furthermore, SIRT5 was evaluated by immunofluorescence and western blotting. The results showed that, compared with the CFA group, both thermal and mechanical pain thresholds increased with moxibustion and the SIRT5 inhibitor MC3482 intervention at ST36. Additionally, compared to the CFA-induced group, the inflammatory mediators, including IL-1β and TNF-α, decreased, while the anti-inflammatory cytokines IL-4 and TGF-β increased with moxibustion and MC3482 ST36 acupoint injection. Western blot results showed a decreased expression of SIRT5 at the ST36 site with moxibustion and MC3482 injection, compared to the CFA-induced group. SIRT5 expression in the right paw of mice injected with moxibustion and MC3482 was higher than that in the CFA-induced group. This study revealed that SIRT5 expression is involved in moxibustion analgesia and may be a potential mediator in the regulation of analgesia.http://www.sciencedirect.com/science/article/pii/S2405844023049733MoxibustionAnalgesiaInflammatory painSIRT5 |
| spellingShingle | Chuan-yi Zuo Chun-yan Gou Cheng-shun Zhang Xi Zhou Peng Lv Han-xiao Zhang Zheng-peng Fan Feng-wei Tian Zhu-xing Wang Role of SIRT5 in the analgesic effectiveness of moxibustion at ST36 in mice with inflammatory pain Heliyon Moxibustion Analgesia Inflammatory pain SIRT5 |
| title | Role of SIRT5 in the analgesic effectiveness of moxibustion at ST36 in mice with inflammatory pain |
| title_full | Role of SIRT5 in the analgesic effectiveness of moxibustion at ST36 in mice with inflammatory pain |
| title_fullStr | Role of SIRT5 in the analgesic effectiveness of moxibustion at ST36 in mice with inflammatory pain |
| title_full_unstemmed | Role of SIRT5 in the analgesic effectiveness of moxibustion at ST36 in mice with inflammatory pain |
| title_short | Role of SIRT5 in the analgesic effectiveness of moxibustion at ST36 in mice with inflammatory pain |
| title_sort | role of sirt5 in the analgesic effectiveness of moxibustion at st36 in mice with inflammatory pain |
| topic | Moxibustion Analgesia Inflammatory pain SIRT5 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023049733 |
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