Recombinant expression of monovalent and bivalent anti-TNT-antibodies: Evaluation of different expression systems
Monoclonal 11B3 anti-TNT (trinitrotoluene) antibody was expressed as a monovalent and bivalent form using different prokaryotic and eukaryotic expression systems. Recombinant expression in Escherichia coli, mammalian cells and the methylotrophic yeast Pichia pastoris was performed to obtain disulfid...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2008-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2008/0352-51390802139S.pdf |
| _version_ | 1818864694952198144 |
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| author | Simonović Mladen Zlatanović-Milošević Svetlana Vrvić Miroslav M. Simonović Branislav |
| author_facet | Simonović Mladen Zlatanović-Milošević Svetlana Vrvić Miroslav M. Simonović Branislav |
| author_sort | Simonović Mladen |
| collection | DOAJ |
| description | Monoclonal 11B3 anti-TNT (trinitrotoluene) antibody was expressed as a monovalent and bivalent form using different prokaryotic and eukaryotic expression systems. Recombinant expression in Escherichia coli, mammalian cells and the methylotrophic yeast Pichia pastoris was performed to obtain disulfide-linked and glycosylated antibody forms. The generation of antibody and subsequent evaluation of the expression rates were performed using intracellular, excretory and periplasmatic expression techniques. All methods involved striving for native expressed antibody with maintenance of its functionality only. |
| first_indexed | 2024-12-19T10:35:44Z |
| format | Article |
| id | doaj.art-f4f29a8ce8b6492685f2303c5a51677f |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-19T10:35:44Z |
| publishDate | 2008-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-f4f29a8ce8b6492685f2303c5a51677f2022-12-21T20:25:38ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212008-01-0173213914510.2298/JSC0802139S0352-51390802139SRecombinant expression of monovalent and bivalent anti-TNT-antibodies: Evaluation of different expression systemsSimonović Mladen0Zlatanović-Milošević Svetlana1Vrvić Miroslav M.2Simonović Branislav3Institut za opštu i fizičku hemiju, BeogradPrirodno-matematički fakultet, KragujevacHemijski fakultet, BeogradInstitut za opštu i fizičku hemiju, BeogradMonoclonal 11B3 anti-TNT (trinitrotoluene) antibody was expressed as a monovalent and bivalent form using different prokaryotic and eukaryotic expression systems. Recombinant expression in Escherichia coli, mammalian cells and the methylotrophic yeast Pichia pastoris was performed to obtain disulfide-linked and glycosylated antibody forms. The generation of antibody and subsequent evaluation of the expression rates were performed using intracellular, excretory and periplasmatic expression techniques. All methods involved striving for native expressed antibody with maintenance of its functionality only.http://www.doiserbia.nb.rs/img/doi/0352-5139/2008/0352-51390802139S.pdftntantibodyrecombinant expression11b3scfv |
| spellingShingle | Simonović Mladen Zlatanović-Milošević Svetlana Vrvić Miroslav M. Simonović Branislav Recombinant expression of monovalent and bivalent anti-TNT-antibodies: Evaluation of different expression systems Journal of the Serbian Chemical Society tnt antibody recombinant expression 11b3 scfv |
| title | Recombinant expression of monovalent and bivalent anti-TNT-antibodies: Evaluation of different expression systems |
| title_full | Recombinant expression of monovalent and bivalent anti-TNT-antibodies: Evaluation of different expression systems |
| title_fullStr | Recombinant expression of monovalent and bivalent anti-TNT-antibodies: Evaluation of different expression systems |
| title_full_unstemmed | Recombinant expression of monovalent and bivalent anti-TNT-antibodies: Evaluation of different expression systems |
| title_short | Recombinant expression of monovalent and bivalent anti-TNT-antibodies: Evaluation of different expression systems |
| title_sort | recombinant expression of monovalent and bivalent anti tnt antibodies evaluation of different expression systems |
| topic | tnt antibody recombinant expression 11b3 scfv |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2008/0352-51390802139S.pdf |
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