GE23077 binds to the RNA polymerase ‘i’ and ‘i+1’ sites and prevents the binding of initiating nucleotides

GE23077 binds to the RNA polymerase ‘i’ and ‘i+1’ sites and prevents the binding of initiating nucleotides

Using a combination of genetic, biochemical, and structural approaches, we show that the cyclic-peptide antibiotic GE23077 (GE) binds directly to the bacterial RNA polymerase (RNAP) active-center ‘i’ and ‘i+1’ nucleotide binding sites, preventing the binding of initiating nucleotides, and thereby pr...

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Bibliographic Details
Main Authors: Yu Zhang, David Degen, Mary X Ho, Elena Sineva, Katherine Y Ebright, Yon W Ebright, Vladimir Mekler, Hanif Vahedian-Movahed, Yu Feng, Ruiheng Yin, Steve Tuske, Herbert Irschik, Rolf Jansen, Sonia Maffioli, Stefano Donadio, Eddy Arnold, Richard H Ebright
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2014-04-01
Series:eLife
Subjects:
RNA polymerase
RNA polymerase-promoter open complex
transcription
transcription initiation
inhibitor
bipartite inhibitor
Online Access:https://elifesciences.org/articles/02450

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