High-throughput screening of tick-borne pathogens in Europe
Due to increased travel, climatic, and environmental changes, the incidence of tick-borne disease in both humans and animals is increasing throughout Europe. Therefore, extended surveillance tools are desirable. To accurately screen tick-borne pathogens, a large scale epidemiological study was condu...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-07-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcimb.2014.00103/full |
| _version_ | 1818559855093350400 |
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| author | Lorraine eMichelet Sabine eDelannoy Elodie eDevillers Gérald eUmhang Anna eAspan Mikael eJuremalm Jan eChirico Fimme Jan van der Wal Hein eSprong Thomas Peter Boye Pihl Kirstine eKlitgaard René eBødker Patrick eFach Sara eMoutailler |
| author_facet | Lorraine eMichelet Sabine eDelannoy Elodie eDevillers Gérald eUmhang Anna eAspan Mikael eJuremalm Jan eChirico Fimme Jan van der Wal Hein eSprong Thomas Peter Boye Pihl Kirstine eKlitgaard René eBødker Patrick eFach Sara eMoutailler |
| author_sort | Lorraine eMichelet |
| collection | DOAJ |
| description | Due to increased travel, climatic, and environmental changes, the incidence of tick-borne disease in both humans and animals is increasing throughout Europe. Therefore, extended surveillance tools are desirable. To accurately screen tick-borne pathogens, a large scale epidemiological study was conducted on 7050 Ixodes ricinus nymphs collected from France, Denmark, and the Netherlands using a powerful new high-throughput approach. This advanced methodology permitted the simultaneous detection of 25 bacterial, and 12 parasitic species (including; Borrelia, Anaplasma, Ehrlichia, Rickettsia, Bartonella, Candidatus Neoehrlichia, Coxiella, Francisella, Babesia, and Theileria genus) across 94 samples. We successfully determined the prevalence of expected (Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Rickettsia helvetica, Candidatus Neoehrlichia mikurensis, Babesia divergens, Babesia venatorum), unexpected (Borrelia miyamotoi) and rare (Bartonella henselae) pathogens in the three European countries. Moreover we detected Borrelia spielmanii, Borrelia miyamotoi, Babesia divergens, and Babesia venatorum for the first time in Danish ticks. This surveillance method represents a major improvement in epidemiological studies, able to facilitate comprehensive testing of tick-borne pathogens, and which can also be customized to monitor emerging diseases. |
| first_indexed | 2024-12-14T00:30:57Z |
| format | Article |
| id | doaj.art-f4f8a1a9647e48dc9a6d88afd83e5660 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-14T00:30:57Z |
| publishDate | 2014-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-f4f8a1a9647e48dc9a6d88afd83e56602022-12-21T23:24:52ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882014-07-01410.3389/fcimb.2014.0010399480High-throughput screening of tick-borne pathogens in EuropeLorraine eMichelet0Sabine eDelannoy1Elodie eDevillers2Gérald eUmhang3Anna eAspan4Mikael eJuremalm5Jan eChirico6Fimme Jan van der Wal7Hein eSprong8Thomas Peter Boye Pihl9Kirstine eKlitgaard10René eBødker11Patrick eFach12Sara eMoutailler13ANSESANSESANSESANSESNational Veterinary Institute (SVA)National Veterinary InstituteNational Veterinary InstituteCentral Veterinary Institute (CVI)National Institute for Public Health and Environment (RIVM)DTUDTUDTUANSESANSESDue to increased travel, climatic, and environmental changes, the incidence of tick-borne disease in both humans and animals is increasing throughout Europe. Therefore, extended surveillance tools are desirable. To accurately screen tick-borne pathogens, a large scale epidemiological study was conducted on 7050 Ixodes ricinus nymphs collected from France, Denmark, and the Netherlands using a powerful new high-throughput approach. This advanced methodology permitted the simultaneous detection of 25 bacterial, and 12 parasitic species (including; Borrelia, Anaplasma, Ehrlichia, Rickettsia, Bartonella, Candidatus Neoehrlichia, Coxiella, Francisella, Babesia, and Theileria genus) across 94 samples. We successfully determined the prevalence of expected (Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, Rickettsia helvetica, Candidatus Neoehrlichia mikurensis, Babesia divergens, Babesia venatorum), unexpected (Borrelia miyamotoi) and rare (Bartonella henselae) pathogens in the three European countries. Moreover we detected Borrelia spielmanii, Borrelia miyamotoi, Babesia divergens, and Babesia venatorum for the first time in Danish ticks. This surveillance method represents a major improvement in epidemiological studies, able to facilitate comprehensive testing of tick-borne pathogens, and which can also be customized to monitor emerging diseases.http://journal.frontiersin.org/Journal/10.3389/fcimb.2014.00103/fullEuropeMolecular Epidemiologysurveillancetick borne diseasesmicrofluidic analyses |
| spellingShingle | Lorraine eMichelet Sabine eDelannoy Elodie eDevillers Gérald eUmhang Anna eAspan Mikael eJuremalm Jan eChirico Fimme Jan van der Wal Hein eSprong Thomas Peter Boye Pihl Kirstine eKlitgaard René eBødker Patrick eFach Sara eMoutailler High-throughput screening of tick-borne pathogens in Europe Frontiers in Cellular and Infection Microbiology Europe Molecular Epidemiology surveillance tick borne diseases microfluidic analyses |
| title | High-throughput screening of tick-borne pathogens in Europe |
| title_full | High-throughput screening of tick-borne pathogens in Europe |
| title_fullStr | High-throughput screening of tick-borne pathogens in Europe |
| title_full_unstemmed | High-throughput screening of tick-borne pathogens in Europe |
| title_short | High-throughput screening of tick-borne pathogens in Europe |
| title_sort | high throughput screening of tick borne pathogens in europe |
| topic | Europe Molecular Epidemiology surveillance tick borne diseases microfluidic analyses |
| url | http://journal.frontiersin.org/Journal/10.3389/fcimb.2014.00103/full |
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